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Visible foliar injury caused by ozone alters the relationship between SPAD meter readings and chlorophyll concentrations in cutleaf coneflower

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Abstract

The ability of the SPAD-502 chlorophyll meter to quantify chlorophyll amounts in ozone-affected leaves of cutleaf coneflower (Rudbeckia laciniata var. digitata) was assessed in this study. When relatively uninjured leaves were measured (percent leaf area affected by stipple less than 6%), SPAD meter readings were linearly related to total chlorophyll with an adjusted R 2 of 0.84. However, when leaves with foliar injury (characterized as a purple to brownish stipple on the upper leaf surface affecting more than 6% of the leaf area) were added, likelihood ratio tests showed that it was no longer possible to use the same equation to obtain chlorophyll estimations for both classes of leaves. Either an equation with a common slope or a common intercept was necessary. We suspect several factors are involved in altering the calibration of the SPAD meter for measuring chlorophyll amounts in visibly ozone-injured leaves, with the most likely being changes in either light absorption or scattering resulting from tissue necrosis.

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References

  • Azia F, Stewart KA, 2001. Relationships between extractable chlorophyll and SPAD values in muskmelon leaves J Plant Nut 24:961-966

    Article  CAS  Google Scholar 

  • Bindi M, Hacour H, Vandermeiren K, Craigon J, Ojanpera K, Sellden G, Hogey P, Finnan J, Fibbi L, 2002. Chlorophyll concentration of potatoes grown under elevated carbon dioxide and/or ozone concentrations Eur J Agron 17:319-335

    Article  CAS  Google Scholar 

  • Carter GA, Mitchell RJ, Chappelka AH, Brewer CH, 1992. Response of leaf spectral reflectance in loblolly pine to increased atmospheric ozone and precipitation acidity J Exp Bot 43:577–584

    Article  CAS  Google Scholar 

  • Carter GA, Rebbeck J, Percy KE, 1995. Leaf optical properties in Liriodendron tulipifera and Pinus strobus as influenced by increased atmospheric ozone and carbon dioxide Can J Forest Res 25:407-412

    Article  CAS  Google Scholar 

  • Castelli F, Contillo R, Miceli F, 1996. Non-destructive determination of leaf chlorophyll content in four crop species J Agron Crop Sci 177:275-283

    Article  CAS  Google Scholar 

  • Chang S, Robison DJ, 2003. Nondestructive and rapid estimation of hardwood foliar nitrogen status using the SPAD-502 chlorophyll meter Forest Ecol Manage 181:331-338

    Article  Google Scholar 

  • Chappelka AH, Neufeld HS, Davison AW, Somers GL, 2003. Evaluation of ozone injury on foliage of cutleaf coneflower (Rudbeckia laciniata) and crownbeard (Verbesina occidentalis) in Great Smoky Mountains National Park Environ Pollut 125:53–59

    Article  CAS  PubMed  Google Scholar 

  • Costa C, Dwyer LM, Dutilleul P, Stewart DW, Ma LB, Smith DL, 2001. Inter-relationships of applied nitrogen, SPAD, and yield of leafy and non-leafy maize genotypes J Plant Nut 24:1173–1194

    Article  CAS  Google Scholar 

  • Davison AW, Neufeld HS, Chappelka AH, Kirsten Wolff, Finkelstein PL, 2003. Interpreting spatial variation in ozone symptoms shown by cutleaf coneflower, Rudbeckia laciniata L Environ Pollut 125:61–70

    Article  CAS  PubMed  Google Scholar 

  • Earl HJ, Tollenaar M, 1997. Maize leaf absorptance of photosynthetically active radiation and its estimation using a chlorophyll meter Crop Sci 37:436-440

    Google Scholar 

  • Evans LS, Adamski JH, Renfro JR, 1996. Relationships between cellular injury, visible injury of leaves, and ozone exposure levels for several dicotyledonous plant species at Great Smoky Mountains National Park Environ Exp Bot 36:229-237

    Article  CAS  Google Scholar 

  • Fanizza G, Ricciardi L, Bagnulo C, 1991. Leaf greenness measurements to evaluate water stressed genotypes in Vitis vinifera Euphytica 55:27–32

    Article  Google Scholar 

  • Finkelstein PL, Davison AW, Neufeld HS, Meyers TP, Chappelka AH, 2004. Sub-canopy deposition of ozone in a stand of cutleaf coneflower Environ Pollut 131:295–303

    Article  CAS  PubMed  Google Scholar 

  • Finnan JM, Burke JI, Jones MB, 1997. A note on a non-destructive method of chlorophyll determination in wheat (Triticum aestivum L.) Ir J Agric Food Res 36:85-89

    Google Scholar 

  • Gratani L, 1992. A non-destructive method to determine chlorophyll content of leaves Photosynthetica 26:469–473

    CAS  Google Scholar 

  • Horsfall JG, Barratt RW, 1945. An improved grading system for measuring plant disease Pytopathology 35:655

    Google Scholar 

  • Krupa SV, McGrath MT, Andersen CP, Booker FL, Burkey KO, Chappelka AH, Chevone BI, Pell EJ, Zilinskas BA, 2000. Ambient ozone and plant health Plant Disease 85:4-12

    Article  Google Scholar 

  • Kumar RR, Marimuthu S, Jayakumar D, Jeyaramraja PR, 2002. In situ estimation of leaf chlorophyll and its relationship with photosynthesis in tea Ind J Plant Physiol 7:367-371

    Google Scholar 

  • Lawson T, Craigon J, Tulloch AM, Black CR, Colls JJ, Landon G, 2001. Photosynthetic responses to elevated CO2 and ozone in field-grown potato (Solanum tuberosum) J Plant Physiol 158:309-323

    Article  CAS  Google Scholar 

  • Ma BL, Morrison MJ, Voldeng HD, 1995. Leaf greenness and photosynthetic rates in soybean Crop Sci 35:1411-1414

    Google Scholar 

  • Manetas Y, Grammatikopoulos G, Kyparissis A, 1998. The use of the portable, non-destructive, SPAD-502 (Minolta) chlorophyll meter with leaves of varying trichome density and anthocyanin content J Plant Physiol 153:513-516

    CAS  Google Scholar 

  • Markwell J, Osterman JC, Mitchell JL, 1995. Calibration of the Minolta SPAD-502 leaf chlorophyll meter Photosynth Res 46:467-472

    Article  CAS  Google Scholar 

  • Monje OA, Bugbee B, 1992. Inherent limitations of nondestructive chlorophyll meters a comparison of two types of meters Hortscience 27:69-70

    CAS  PubMed  Google Scholar 

  • Neter J, Wasserman W and Kutner M (1990) Applied Linear Statistical Models, 3rd edn. Richard D Irwin, Inc, Boston, MA

  • Ommen OE, Donnelly A, Vanhoutvin S, van Oijen M, Manderscheid R, 1999. Chlorophyll content of spring wheat flag leaves grown under elevated CO2 concentrations and other environmental stresses within the ‘ESPACE-wheat’ project Eur J Agron 10:197-203

    Article  Google Scholar 

  • Peñuelas J, Filella I, 1998. Visible and near-infra-red reflectance techniques for diagnosing plant physiological status Trends Plant Sci 3:151–156

    Article  Google Scholar 

  • Peñuelas J, Munné-Bosch S, Filella I, 2004. Leaf reflectance and photo- and antioxidant protection in field-grown summer-stressed Phillyrea angustifolia Optical Signals of Oxidative Stress? New Phytologist 162:115–124

    Article  Google Scholar 

  • Porra RJ, Thompson WA, Kriedemann PE, 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents verification of the concentration of chlorophyll standards by atomic absorption spectroscopy Biochim Biophys Acta 975:384-394

    Article  CAS  Google Scholar 

  • Samdur MY, Singh AL, Mathur RK, Manivel P, Chikani BM, Gor HK, Khan MA, 2000. Field evaluation of chlorophyll meter for screening groundnut (Arachis hypogaea L.) genotypes tolerant to iron-deficiency chlorosis Curr Sci Bangalore 79:211-214

    Google Scholar 

  • SAS Institute, Inc. 1999. SAS/STAT User’s Guide, Vers. 8.2, Cary, NC

  • Tenga AZ, Ormrod DP, 1990. Diminished greenness of tomato leaves exposed to ozone and post-exposure recovery of greenness Environ Pollut 64:29-42

    Article  CAS  PubMed  Google Scholar 

  • Tonneijck AEG, van Dijk CJ, 2002. Injury and growth response of subterranean clover to ambient ozone as assessed by using ethylenediurea (EDU): three years of plant monitoring at four sites in The Netherlands Environ Exp Bot 48:33-41

    Article  CAS  Google Scholar 

  • Wellburn AR, 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents and spectrophotometers of different resolution J Plant Physiol 144:307-313

    CAS  Google Scholar 

  • Yamamoto A, Nakamura T, Adu-Gyamfi JJ, Saigusa M, 2002. Relationship between chlorophyll content in leaves of sorghum and pigeonpea determined by extraction method and by chlorophyll meter (SPAD-502) J Plant Nut 25:2295-2301

    Article  CAS  Google Scholar 

  • Young MJ, Berguson WE, Nelson ND, 2003. In situ foliar nitrogen determination in hybrid poplar plantations using a Minolta SPAD-502 chlorophyll meter Physiol Mol Biol Plants 9:261-264

    Google Scholar 

Download references

Acknowledgements

We would like to thank Susan Sachs and Paul Super, rangers at Purchase Knob, for their assistance with this research. In addition, we appreciate the comments of the anonymous reviewers, which greatly helped to improve this paper. This work was made possible by grants from the National Park Service (PMIS66941), and Appalachian State University.

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Authors and Affiliations

  1. Department of Biology, Appalachian State University, 572 Rivers St., Boone, NC, 28608, USA

    Howard S. Neufeld

  2. School of Forestry and Wildlife Sciences, Auburn University, 108 M. White-Smith Hall, Auburn, AL, 36849, USA

    Arthur H. Chappelka & Greg L. Somers

  3. USDA-ARS Plant Science Research Unit, 3127 Ligon Street, Raleigh, NC, 27607, USA

    Kent O. Burkey

  4. Department of Biological Sciences, University of Newcastle, Newcastle, NE1 7RU, UK

    Alan W. Davison

  5. Atmospheric Modeling Division, NOAA: on assignment to US EPA, Research Triangle Park, NC, 27711, USA

    Peter L. Finkelstein

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  1. Howard S. Neufeld
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  2. Arthur H. Chappelka
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  3. Greg L. Somers
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  4. Kent O. Burkey
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  5. Alan W. Davison
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  6. Peter L. Finkelstein
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Correspondence to Howard S. Neufeld.

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Neufeld, H.S., Chappelka, A.H., Somers, G.L. et al. Visible foliar injury caused by ozone alters the relationship between SPAD meter readings and chlorophyll concentrations in cutleaf coneflower. Photosynth Res 87, 281–286 (2006). https://doi.org/10.1007/s11120-005-9008-x

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  • DOI: https://doi.org/10.1007/s11120-005-9008-x

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