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Ectopic Expression of Erigeron breviscapus JAZ1 Affects JA-Induced Development Processes in Transgenic Arabidopsis

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Abstract

The jasmonate ZIM-domain (JAZ) proteins are repressors that function in the regulation of plant growth, development, and response to stimulation of different signals in the JA signaling pathway. Erigeron breviscapus is characteristic of sporophyte self-incompatibility (SSI). However, whether JA signaling is involved in regulation of development processes in E. breviscapus is unclear. In this study, the JAZ homolog EbJAZ1 was isolated and characterized from E. breviscapus. EbJAZ1 was localized to the nucleus, and expressed in roots, stems, leaves and flowers. Ectopic expression of EbJAZ1 in Arabidopsis resulted in shorter filament and silique length, and lower seed fertility. In addition, MeJA-induced root growth inhibition was compromised in transgenic plants. Further qRT-PCR analysis indicated that expression patterns of marker genes for VSP1, VSP2, JAZ1, JAZ5, JAZ8, JAZ10, MYC2, and bHLH17 were downregulated in transgenic plants compared to wild-type, suggesting that EbJAZ regulates the development of flower organs, seed fertility, and primary root growth through the JA signaling pathway. Thus, our results indicate that EbJAZ1 is one of the important regulators possibly involved in SSI and other developmental processes in Erigeron breviscapus.

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Funding

This work was supported by the National Natural Sciences Foundation of China (Nos. 81760692, 81960684), the Major Science and Technique Programs in Yunnan province (No. 2017ZF002), and the special fund project for basic research of local universities in Yunnan Province (No. 2018FH001-011).

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Authors and Affiliations

  1. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China

    Mo Chen, Mi Yan, Simei He, Yingchun Lu, Guanghui Zhang, Bing Hao, Wei Fan & Shengchao Yang

  2. National and Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, 650201, China

    Mo Chen, Mi Yan, Simei He & Shengchao Yang

  3. Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, 650223, China

    Mo Chen, Mi Yan & Yanru Hu

  4. Maotai Institute, Renhuai, 564516, China

    Mo Chen

  5. Honghe University, Mengzi, 661100, China

    Wei Zhang

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  1. Mo Chen
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  2. Wei Zhang
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Contributions

YRH and SCY conceived and designed the research; MC and MY conducted the experiments; WZ, SMH, and YCL analyzed the data and created the figures; GHZ, BH, and FW performed the statistical analysis; MC and WF wrote the manuscript. All authors read and approved the manuscript.

Corresponding authors

Correspondence to Yanru Hu or Shengchao Yang.

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The authors declare no competing interests.

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Key message

• EbJAZ1 is one of the important regulators involved in developmental processes in Erigeron breviscapus.

• The ectopic expression of EbJAZ1 can regulate the development of flower organs, seed fertility, and primary root growth through the JA signaling pathway.

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Chen, M., Zhang, W., Yan, M. et al. Ectopic Expression of Erigeron breviscapus JAZ1 Affects JA-Induced Development Processes in Transgenic Arabidopsis. Plant Mol Biol Rep 40, 530–538 (2022). https://doi.org/10.1007/s11105-021-01289-4

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  • DOI: https://doi.org/10.1007/s11105-021-01289-4

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