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Salinity response pattern and isolation of catalase gene from halophyte plant Aeluropus littoralis

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Photosynthetica

Abstract

The role of the antioxidant defense system in salt tolerance of Aeluropus littoralis has not been yet reported; therefore in the present study, the changes of catalase (CAT) activity in this halophyte plant was investigated and CAT gene was isolated. The leaves of treated and control plants were harvested at various times, starting 1 day prior to initiating treatment, then periodically at 72-h intervals for 21 days. The data collected showed that CAT activity increased significantly with time in plants treated with 200, 400, and 600 mM NaCl when compared with the control plants. Maximum enzyme activity was observed between the 6th and 12th day at all NaCl concentrations. CAT gene was isolated and cloned via pTZ57R/T cloning vector in Escherichia coli. CAT gene encoded 494 amino acids and had also high homology of 90, 87, 86, and 86% with CAT genes from Zea mays, Oryza sativa, Triticum aestivum, and Hordeum vulgare, respectively.

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Abbreviations

Car(s):

carotenoid(s)

CAT:

catalase

Chl:

chlorophyll

DTT:

dithiothreitol

EDTA:

ethylenediaminetetraacetic acid

ORF:

open reading frame

PMSF:

phenyl methanesulfonyl fluoride

POD:

peroxidase

PVP:

polyvinylpyrrolidone

ROS:

reactive oxygen species

RWC:

relative water content

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Authors and Affiliations

  1. Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, 14115 - 336, I. R. Iran

    M. Modarresi

  2. Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, 578, I.R. Iran

    G. A. Nematzadeh

  3. Basic Sciences Group, Sari Agricultural Sciences and Natural Resources University, Sari, 578, I.R. Iran

    F. Moradian

Authors
  1. M. Modarresi
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  2. G. A. Nematzadeh
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  3. F. Moradian
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Correspondence to M. Modarresi.

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Acknowledgment: This work was supported by Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari University of Agricultural Sciences and Natural Resources. The authors thank Dr. H. Pirdashti and Mr. A. Heidarzadeh (Laboratory of Environmental Stress) and Mrs. Alamdar (Laboratory of Chemistry) for their assistance and companionship.

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Modarresi, M., Nematzadeh, G.A. & Moradian, F. Salinity response pattern and isolation of catalase gene from halophyte plant Aeluropus littoralis . Photosynthetica 51, 621–629 (2013). https://doi.org/10.1007/s11099-013-0060-z

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  • DOI: https://doi.org/10.1007/s11099-013-0060-z

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