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Extraction, composition and the antioxidant and anticomplement activities of high molecular weight fractions from the leaves of Symphytum asperum and S. caucasicum

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Pharmaceutical Chemistry Journal Aims and scope

High molecular weight fractions (> 1000 kDal) from the leaves of Symphytum asperum and S. caucasicum had anticomplement activity, were able to inhibit luminol- and lucigenin-dependent chemiluminescence (CLlum and CLluc respectively) induced by human polymorphonuclear neutrophils (PMN) after stimulation with opsonized zymosan, and could decrease the CLluc signal induced by superoxide anion formation in a cell-free hypoxanthine/xanthine oxidase system. The anticomplement and antioxidant activities of the high molecular weight fractions (> 1000 kDal) from the leaves of Symphytum asperum and S. caucasicum indicate that they might be used as anti-inflammatory and wound-healing agents.

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References

  1. G. Abuladze, V. Barbakadze, and K. Mulkijanyan, Proc. Georgian Acad. Sci., Biol. Ser., 21(16), 129 – 132 (1995).

    Google Scholar 

  2. V. V. Barbakadze, E. P. Kemertelidze, A. I. Usov, et al., Proc. Georgian Acad. Sci. Biol. Ser., 25(4 – 6), 207 – 216 (1999).

    Google Scholar 

  3. V. Barbakadze, É. Kemertelidze, I. Targamadze, et al., Khim. Prirod. Soedin., No. 4, 303 – 305 (2005).

  4. V. V. Barbakadze, E. P. Kemertelidze, A. S. Shashkov, and A. I. Usov, Mendeleev Commun., 10(4), 148 – 149 (2000).

    Article  Google Scholar 

  5. V. V. Barbakadze, É. P. Kemertelidze, I. L. Targamadze, et al., Bioorgan. Khim., 28(4), 362 – 366 (2002).

    CAS  Google Scholar 

  6. V. Barbakadze, E. Kemertelidze, I. Targamadze, et al., Molecules, 10(9), 1135 – 1144 (2005).

    Article  PubMed  CAS  Google Scholar 

  7. V. V. Barbakadze, Eh. P. Kemertelidze, K. G. Mulkidzhanyan, et al., Khim.-Farm. Zh., 41(1), 14 – 17 (2007).

    CAS  Google Scholar 

  8. C. M. Barthomeuf, E. Debiton, V. V. Barbakadze, and E. P. Kemertelidze, J. Agric. Food Chem., 49(8), 3942 – 3946 (2001).

    Article  CAS  Google Scholar 

  9. M. B. Hampton, A. J. Kettle, and C. C. Winterbourn, Blood, 92(9), 3007 – 3017 (1998).

    PubMed  CAS  Google Scholar 

  10. B. M. Babior, Curr. Opin. Hematol., 2, 55 – 60 (1995).

    Article  PubMed  CAS  Google Scholar 

  11. S. J. Weiss, New Engl. J. Med., 320(6), 320 – 376 (1989).

    Google Scholar 

  12. B. Latha, and M. Babu, Burns, 27, 709 – 317 (2001).

    Article  Google Scholar 

  13. I. Roitt, J. Brostoff, and D. Male, in: Immunology: Complement, Mosby, London, Madrid, etc. (1996), Fourth Edition, 13.1 – 13.17.

  14. Ts. N. Gviniashvili, in: The Caucasian Species of the Genus Symphytum L., Metsniereba, Tbilisi (1976), pp. 130 – 135.

  15. F. M. van den Dungen, in: Ph. D. Thesis: Symphytum, officinale L.: Influence on Immune Functions and Wound-Healing Processes, Utrecht University, Utrecht (1993).

  16. V. V. Barbakadze, R. A. Gakhokidze, Z. S. Shengeliya, and A. I. Usov, Khim. Prirod. Soedin., No. 3, 330 – 335 (1989); [Chem. Nat. Compd. (Engl. Transl.), 25(3), 281 – 286 (1989)].

  17. V. V. Barbakadze, É. P. Kemertelidze, G. E. Dekanosidze, et al., Bioorgan. Khim., 18(5), 671 – 679 (1992).

    CAS  Google Scholar 

  18. W. Yaphe and G. P. Arsenault, Anal. Biochem., 13(1), 143 – 148 (1965).

    Article  CAS  Google Scholar 

  19. N. Blumenkrantz and G. Asboe-Hansen, Anal. Biochem., 54(2), 484 – 489 (1973).

    Article  PubMed  CAS  Google Scholar 

  20. D. Roos, A. A. M. Bot, M. L. J. van Schaik, et al., J. Immunol., 126(2), 433 – 440 (1981).

    PubMed  CAS  Google Scholar 

  21. E. van denWorm, C. J. Beukelman, A. J. J. van den Berg, et al., Eur. J. Pharmacol., 433, 225 – 230 (2001).

    Article  PubMed  Google Scholar 

  22. H. A. Verbrugh, R. Peters, P. K. Peterson, and J. Verhoef, J. Clin. Pathol., 31, 539 – 545 (1978).

    Article  PubMed  CAS  Google Scholar 

  23. J. P. A. M. Klerx, C. J. Beukelman, H. van Dijk, and J. M. N. Willers, J. Immunol. Meth., 63, 215 – 220 (1983).

    Article  CAS  Google Scholar 

  24. J. M. Simons, L. A.’t Hart, H. van Dijk, et al., J. Ethnopharmacol., 26, 169 – 182 (1989).

    Article  PubMed  CAS  Google Scholar 

  25. K. M. Markham, in: Techniques of Flavonoid Identification, Academic Press, London, New York, etc. (1982), pp. 72 – 78.

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This study was supported by the Georgian-American Bilateral Grant Program III 2006 (No. GEB2-3344-TB-06).

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Authors and Affiliations

  1. I. G. Kutateladze Institute of Pharmacology, Tbilisi, Georgia

    V. V. Barbakadze, K. G. Mulkidzhanyan, M. I. Merlani, L. M. Gogilashvili, L. Sh. Amiranashvili & E. K. Shaburishvili

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  1. V. V. Barbakadze
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  2. K. G. Mulkidzhanyan
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  3. M. I. Merlani
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  4. L. M. Gogilashvili
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  5. L. Sh. Amiranashvili
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  6. E. K. Shaburishvili
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Correspondence to V. V. Barbakadze.

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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 44, No. 11, pp. 18 – 21, November, 2010.

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Barbakadze, V.V., Mulkidzhanyan, K.G., Merlani, M.I. et al. Extraction, composition and the antioxidant and anticomplement activities of high molecular weight fractions from the leaves of Symphytum asperum and S. caucasicum . Pharm Chem J 44, 604–607 (2011). https://doi.org/10.1007/s11094-011-0527-9

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  • DOI: https://doi.org/10.1007/s11094-011-0527-9

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