Highly sensitive V-shaped SPR PCF biosensor for cancer detection

In this paper, a novel design of photonic crystal fiber (PCF) biosensor based on surface plasmon resonance (SPR) is introduced and analyzed for cancer cell detection. The full vectorial finite element method (FVFEM) is used throughout the numerical analysis of the suggested biosensor. The reported PCF has a V-shaped surface that is coated with ZrN as a plasmonic material. A coupling occurs between the core guided mode and surface plasmon mode SPM which depends on the studied analyte. Such a coupling is improved by using the suggested V-shape geometry which increases the sensor sensitivity.The geometrical parameters are optimized to achieve high sensor sensitivity. The proposed biosensor has high optical sensitivity of 6214.28, 3800, and 5008.33 nm/RIU, for quasi-transverse magnetic (TM), and 6000 nm/RIU, 4400 nm/RIU, and 5333.3 nm/RIU, for quasi-transverse magnetic (TE), for breast, basal, and cervical cancer cells, respectively. The reported optical sensor can pave the way for efficient and simple technique for cancer detection with low cost and high sensitivity instead of surgical and chemical techniques.


Introduction
Photonic crystal fiber (PCF) (Hameed et al. 2011) is a distinctive and special class of optical fibers that has attracted a lot of attention due to its advantages with wide range of applications. PCFs have high birefringence (Sharma et al. 2013), (Hameed et al. 2009a, b) large mode area (Raja and Varshney 2014), infinitely single mode operation (Akowuah et al. 2009), tunable dispersion (Hsu et al. 2015), and low loss (Bradley et al. 2013). Therefore, PCFs have been used for different applications such as polarization handling devices (Hameed and Obayya 2010) (Younis et al. 2018), and Jindal et al. have introduced linked photonic crystal (PhC) waveguide cancer detector with sensitivity up to 391 nm/RIU (Jindal et al. 2016).
Conventional plasmonic PCF sensors depend on utilizing silver, gold or bimetallic configuration of gold and silver (Akowuah et al. 2012). The conventional plasmonic materials (Gold and silver) have disadvantages of boarder resonance curves and bad chemical stability, respectively. Thus, new plasmonic materials such as ZrN, TiN, ITO and ZnO are used. The ZrN is refractory plasmonic material with a very high melting point, mechanically strong, abrasion resistant and high chemical stability at temperatures above 2000 °C with CMOS compatibility (El-Saeed et al. 2019). However, ZrN is superior with higher hardness value, less growth rate and texture orientation at higher pulse voltage. (Heinrich et al. 2008), low cost (Monfared, Kurylyk and Dasog, 2022), and best corrosion resistance (Zhang et al. 2022). As a result, ZrN is utilized as a plasmonic material for the proposed device. Further, the reported sensor is externally coated design, which makes it label free, easy to use, more safe, and easy to fabricate.
In recent years, gold∕TiO 2 coated PCF biosensor was presented for breast cancer cells which offers sensitivities of 11,034 nm/RIU and 9,285 nm/RIU for x-and y-polarized core modes, respectively, with resolution in the range of 10 −6 RIU (Verma, Kumar and Jindal, 2022). Further, photonic crystal (DPhC) was suggested for cancerous brain tumors detection, using SiO 2 nanoparticles embedded in a superconducting material layer. The reported biosensor achieves sensitivity of 4139.24 nm/RIU (Malek et al. 2022). Additionally, SPR PCF biosensor with gold (Au) and titanium dioxide ( TiO 2 ) coated layers was proposed for different cancer cell detection with sensitivities of 9428.57 nm/RIU, 10,714.28 nm/RIU, 7571.43 nm/RIU, 5500 nm/RIU, and 6000 nm/RIU for the MDAMB-231, MCF-7, PC12, HeLa, and Jurkat cancerous cells, respectively (Chaudhary, Kumar and Kumar, 2022). Moreover, 1-D binary PhC with a defect layer using ( Si∕SiO 2 N/Defect/(Si∕SiO 2 )N structure was introduced for cancer cells detection with sensitivity of 2400.08 nm/RIU (Almawgani et al. 2022). In addition, RI based sensor for skin cancer (Basal, HeLa, MDA-MB-231) detection using silicon rods in the air bed was presented with sensitivity of 3080 nm/RIU (Sani et al. 2022). Additionally, SPR-PCF biosensor for two breast cancer cells detection was developed with sensitivities of 4500 nm/RIU and 4643 nm/RIU for MDA-MB-231 and MCF-7 cancer cells, respectively (Yasli 2022). Moreover, two dimensional random PCF biosensor for breast cancer cells was proposed with sensitivity of 1372.549 nm/RIU (Nayak 2023). Furthermore, 1D PhC based sensor was reported with achieved sensitivity of 73 nm/RIU for cancer cells (Bijalwan et al. 2021). In addition, plasmonic PCF biosensor for cancer detection of a single living cell was developed with sensitivities of 7142.86 nm/ RIU and − 757 RIU −1 for MCF-7, and Hela cell, respectively (Yasli 2021). Plasmonic PCF breast cancer cells biosensor based on ITO coated layer was introduced with sensitivities of 6701.03 nm/RIU and 5154.63 nm/RIU for x-and y-polarization, respectively (Rajeswari and Revathi 2022). Additionally, bottom side polished PCF based biosensor for early cancer detection was presented with sensitivity of 22,857 nm/RIU for blood cancer, 20000 nm/ RIU for cervical cancer, 20714 nm/RI for adrenal gland cancer, 20000 nm/RIU for skin cancer, and 21,428, 25,000 nm/RIU for breast cancer (type-1and type-2)), respectively (Singh and Prajapati 2023).
In this paper, V-shaped SPR PCF sensor is suggested for cancer early detection. The V-groove shape improves the interaction between the core guided mode and SPR mode at the metal/dielectric interface. This helps detect minute variations in the analytes' refractive indices. (Omri et al. 2020). The reported PCF depends on ZrN as a plasmonic material. It's worth noting that, the optimum ZrN thickness layer at the proposed PCF sensor is equal to 40 nm, which makes the device's cost low with respect to others in literature (Kaur and Singh 2020). Furthermore, dimensions of the sensor are sufficiently large, which makes the fabrication process easier. The geometrical parameters are studied within the allowed fabrication constrains to achieve high sensor sensitivity using full vectorial finite-element method (FVFEM) is used (Obayya et al. 2002). High sensitivities of 6214.28 nm/RIU, 3800 nm/RIU, and 5008.33 nm/RIU, are achieved with resolutions of 2.728 × 10 −5 RIU ,2.63 × 10 −5 RIU , and 1.92 × 10 −5 RIU , for breast, basal and cervical cancer cells, respectively using quasi TM mode. For the quasi TE mode, the suggested design offers corresponding sensitivities of 6000 nm/RIU, 4400 nm/RIU, and 5333.3 nm/RIU, and resolutions of 1.666 × 10 −5 RIU,2.27 × 10 −5 RIU , and 1.875 × 10 −5 . Further, the analyte is housed in an external channel to facilitate the fabrication process. Additionally, the suggested design could be also used to detect the change in RI for different bio-analyte. Moreover, the achieved sensitivity is greater than that has been reported in (Jabin et al. 2019), (Ayyanar et al. 2018), (Ramanujam et al. 2019), (Jindal et al. 2016), (Sani et al. 2022), (Almawgani et al. 2022), (Malek et al. 2022), (Yasli 2022), (Nayak 2023), and (Ramanujam et al. 2019). Figure 1 shows the 2D cross section of the suggested PCF biosensor. A background of silica is used where air holes of radius r = 2.35 μm are arranged in a hexagonal lattice with a hole pitch Λ = 6.73 μm. Further, the upper surface is etched at a distance h = 8.5 μm from the PCF center. The V-groove is then made with a distance x = 13.6 μm between the two vertices of the V-shape. The etched surface is coated by ZrN layer with a thickness t = 30 nm. The studied analyte covers the plasmonic layer as shown in Fig. 1a. In the same context, Fig. 1(b) shows the sensing system where the laser source is coupled to the PCF biosensor through single mode fiber (SMF) (Luo et al. 2019). Further, SMF will also connect the transmitted light to the spectrometer in order to detect the transmission and hence the losses through the suggested device. High-resolution optical spectrum analyzer (OSA) can be used to show the measured data (Koshiba and Saitoh, 2003). The coupling between the core mode and SPM depends on the analyte RI. At a given RI, a maximum peak of confinement loss is obtained. The analyte RI fluctuation can be detected by measuring the shift in the resonance wavelength. The initial geometrical parameters of the suggested design are illustrated in Table 1. The RI of the silica background material is calculated from the following Sellmeier equation (Gamal et al. 2021a, b):

Design considerations
where n s is the RI of the silica, and λ is the wavelength in μm. B 1 , B 2 and B 3 are equal to 0.69616630, 0.40794260, and 0.89747940, respectively. Also C 1 , C 2 and C 3 are given by 0.004679148260, 0.01351206310, and 97.93400250 m 2 , respectively.
The permittivity of the ZrN is calculated by the following Drude model (Naik et al. 2013).
where Im n eff is the imaginary part of the complex effective index of the core guided modes. Moreover, the sensor sensitivity S λ (λ) can be calculated as (Gamal et al. 2021a, b): where λ peak is the resonance wavelength with maximum losses, and n analyte is the analyte RI.
The proposed biosensor can be fabricated using one of several well-known processes (Gupta and Sharma, 2006), including, the sol-gel casting approach (Bise and Trevor, 2005), and drilling (Liu et al. 2010(Liu et al. , 2019. Furthermore, the fused array fabrication approach can be utilized to implement air holes with a radius of 0.1 m or greater in a uniform shape (Falkenstein and Justus, 2013). Through strict process control, the placement of the air holes in the cladding zone could be precisely regulated. In addition, the PCF can be polished in a D-shaped pattern, as reported in (Wang et al. 2009). The D-shaped PCF can be then transformed into a V-shaped cross section as follows. First, a thin silica coating × Im n eff × 10 4 ≈ 8.686 × Im n eff × 10 4 dB cm (4) S λ (λ) = λ peak n analyte is applied to the surface of the D-etched surfaces. Electron-beam lithography can be used to create a slit pattern. Then, HF solution etching will be used to remove the silica film in the slit area. After that, the PCF will be immersed in a 25 percent tetramethylammonium hydroxide (TMAH) solution to etch the V-shaped groove into the silica layer. The initial incision width in the silica mask and the etching duration can be used to control the depth of the V-groove (Migita et al., 2014). Further, the V-groove can be obtained by using wheel polishing technique (Luo et al. 2021) which is more straightforward, and precise. Finally, a high-pressure chemical vapor deposition technique can be used to coat the ZrN layer over the V-shaped PCF structure (Hassani and Skorobogatiy, 2006). Furthermore, analyte penetration on the PCF's outer surface can be accomplished easily by pumping the analyte over the plasmonic material (Kaur and Singh 2020).

Numerical results and discussion
The suggested design is studied and analyzed using COMSOL Multiphysics software package 5.4 (https:// www. comsol. com/) via 2D, radio frequancy, electromagnetic waves, frequancy domain (emw) model. In this study, computational domain of ( 74 m × 74 m ) is used with outer PCF radius of 37 m The computational domain is discredited with total number of elements of 51,956, minimum element size of 0,0223 µm, maximum element size of 4.97 µm and 1,086,496 degrees of freedom. Perfectly matched layer (PML) (Koshiba and Tsuji, 2000) is applied to the transverse directions to truncate the simulation domain. Further, extensive convergence tests are performed to ensure the numerical accuracy and stability of the results for the proposed sensor. The convergence occurs when the PML thickness is ≥ 1.7 µm at wavelength λ = 600 nm. Therefore, a PML with a thickness of 2.0 µm is applied with a scale factor 1. The proposed design is utilized to detect the RI variation of the studied analyte with different types of cancer cells such as; breast, cervical and basal cells. The RI range for such cancer types varies from 1.3919 to 1.4009 while the normal cell varies from 1.35 to 1.37 as shown in Table 2. Figure 2 shows the dispersion of the effective RI of the core mode and SPM and the loss spectrum of the core mode at normal breast cell with n a = 1.385 . It may be seen that maximum loss takes place at a resonance wavelength of 612 nm where maximum power transfer occurs from the core mode to the SP mode. It is also evident from the inset field plot that the core mode has more leakage at the resonance wavelength toward the metal/dielectric interface with more power loss. In this case, the effective indices of the core mode and SPM are equal. In order to achieve high spectral sensitivity, the geometrical parameters of  (Sharan et al. 2014) the biosensor are optimized in the allowed range for the fabrication process, as summarized in Table 3. Figure 3 depicts a comparison between the D-shaped PCF and the suggested V-shape design at r = 2.35 µm, Λ = 6.73 µm, and t = 30 nm where the RI of the analyte changes from n a = 1.385 to n a = 1.399. Figure 3 shows the loss peak and field plots of the quasi TM mode of the V and D shaped PCFs, at λ = 550 nm and 800 nm respectively. It may be seen that a good coupling is achieved using the V-shape design for both analyte refractive indices. On the other hand, the resonance wavelength of the D-shape structure at n a = 1.399 is not strong with broad absorption spectrum. Therefore, V-shaped PCF is suggested with a good coupling between the core mode and SP mode.
In order to increase the sensor sensitivity for breast cancer detection, the geometrical parameters are studied. The effect of the cladding air holes radius (r) is first investigated and depicted in Fig. 4. However, the other parameters are fixed at their initial values, where Ʌ = 6.73 µm, x = 13.6 µm, and t = 30 nm. The studied r values are 2.3 µm, 2.35 µm, and 2.4 µm, while the analyte RI changes from n a = 1.385 to n a = 1.399 for breast cancer cell. It is evident from Fig. 4a, b and Table 3 that the air hole radius has a slight effect on the resonance wavelength of the two polarized modes at different analyte refractive indices. This is due to the well confinement of the two polarized modes in the core region. The resulted resonance wavelength peaks of the quasi TM are slightly red shifted and sensitivity is slightly increased from 4000 nm/RIU to 4071 nm/RIU, while the resonance peak and resonance peak shift are fixed for the quasi TE mode. On the other side, the variation of r has no impact on the SPM's effective index. According to Fig. 4 and Table 3, high sensor sensitivity achieves its maximum value at r = 2.35 μm, which is used in the next study.
The effect of the pitch size Λ is also investigated. Figures 5a, b show the loss spectra of the quasi TM and quasi TE modes, respectively, while the other parameters are equal to r = 2.35 µm, x = 13.6 µm, and t = 30 nm. For quasi TM core mode, with increasing Λ from 6.6 µm, 6.73 µm, the resonance peak at n a = 1.399 is slightly red shifted from λ = 0.668 mtoλ = 0.669 m , and the sensitivity increase from 3928.6 nm/RIU to 4071.4 nm/RIU. This is due to the well confinement of the light in the core region. Therefore, the resonance wave length is slightly affected by the hole pitch variation as shown in   Table 3. It may be seen that high sensitivity is obtained at a hole pitch size (Λ) of 6.73 m , which is taken in the subsequent studies. Since the plasmonic material is responsible for SPM excitation owing to the coupling between the core and SPM. Therefore, the effect of the ZrN layer thickness (t) is investigated. Figures 6a, b show the confinement loss of the quasi TM and quasi TE modes at different thickness values of ZrN layer (20, 30, and 40) nm at n n = 1.385 and n d = 1.399. However, the geometrical parameters are fixed at r = 2.35 µm, x = 13.6 µm, and Λ = 6.73 µm. It is worth noting from Table 3 and Figs. 6a, b that the loss peak has a red shift from λ = 0.545 mtoλ = 0.765 m by increasing the thickness t from 20 to 40 nm. It should be noted that the thickness of the plasmonic material will influence the decay rate of the evanescent wave and SPM. However, the crystallites of ZrN show growth elongation in all directions with increasing deposition temperature. As the thickness is increased by more than 40 nm, the difficulty in the fabrication process will be increased (Sandu et al. 2008). Furthermore, the more increasing ZrN thickness, the weaker plasmonic effect, and the more wide confinement loss curve is obtained (Magdi and Swillam 2017). In this curve, the sharpness is reduced as depicted in Figs. 6a, b. At t = 40 nm, the sensitivity gets its maximum value of 5500 nm/RIU and 5357.14 nm/RIU for the quasi TM and TE modes, respectively. Thus, t is fixed at 40 nm to sustain the maximum sensitivity value.
The impact of the distance x (distance between the two V-shape vertices) is then studied. It is worth mentioning that x reflects the value of angle ( θ) at constant depth (h) of the V-groove geometry. Figure 7 depicts the confinement loss spectra of the quasi TM and quasi TE core modes for different values of x (12.4 µm,13.6 µm, 15.2 µm, 16.6 µm and 18 µm) while the other parameters are taken as r = 2.35 µm, Λ = 6.73 µm, and t = 40 nm. It is worth noting from Fig. 7 (a) and (b) that as the analyte RI changes from n a = 1.385 for normal cell to n a = 1.399 for cancerous cell, the resonance wavelength peaks of the quasi TM and quasi TE modes are red shifted. As the distance x increases, the mode confinement through the core region will also increase. Therefore, the energy transferred to plasmonic material will be increased with improved sensitivity. The maximum sensitivities of 6214.28 nm/RIU and 6000 nm/RIU for the quasi TM and quasi TE core modes, respectively, is achieved at x = 18 µm (θ = 117 • ) which is taken as an optimum value. Due to the intersection between the V-groove sides and the nearby air holes, x value cannot be increased more than 18 µm which is considered a fabrication limit.
Next, the sensor sensitivity for cancer early detection of other types of cells is studied. Figure 8 shows the confinement loss spectra of the two polarized modes using normal cells, and corresponding cancerous cells including breast, basal and cervical cells. It may be seen from Fig. 8 that the proposed biosensor can detect breast, basal, and cervical cancer cells with sensitivities of 6214.28 nm/RIU, 3800 nm/RIU, and 5008.33 nm/RIU, and good resolution of 2.728 × 10 −5 RIU,2.63 × 10 −5 RIU , and 1.92 × 10 −5 RIU , respectively, for the quasi TM core mode. Moreover, the suggested design offers sensitivities of 6000 nm/RIU, 4400 nm/RIU, and 5333.3 nm/RIU, and resolution of 1.666 × 10 −5 RIU,2.27 × 10 −5 RIU , and 1.875 × 10 −5 , respectively, for quasi the TE mode as shown in Table 4.  The linear performance of the proposed design is also investigated. Figure 9a shows the resonance wavelength variation with the analyte RI for quasi TM mode. It is evident that linear fitting can be represented by λ = 4.4 × 10 3 n − 5.4 × 10 3 with coefficient of determination ( R 2 ) and correlation coefficient (r) of 0.9 and 0.95, respectively. Moreover, Fig. 9b depicts the resonance wavelength variation with the RI of the analyte for quasi TE mode where the linear fitting can be represented by λ = 4.3 × 10 3 n − 5.2 × 10 3 with R 2 and r of 0.93 and 0.96, respectively. One of most important parameters for the sensors' quality is the linear relation between the measured value (RI) and its corresponding resonance wavelength. More linearity of the sensor behavior, gives the sensor more accurate measurement along that linear range. The proposed sensor has a good linear response with stable performance. Moreover, it is accurate to predict the resonance wavelength at any given value of refractive index along the RI range from 1.35-1.41.
The resolution of the sensing process is an important metric that can be used to analyze the smallest RI changes. As given by (Gamal et al. 2021a, b).
where Δλ min is the wavelength resolution, Δλ r is the resonance wavelength shift, and Δn a is the analyte RI change. In this work, Δn a is equal to 0.014, Δλ r is 81 and 84 nm for quasi TM, and quasi TE mode, respectively, when the analyte RI changes from 1.385 to 1.399. Assuming Δλ min is 0.1 nm (Wang et al. 2020), high resolution of the reported biosensor will be 2.728 × 10 −5 RIU , and 1.666 × 10 −5 RIU for quasi TM and TE core modes, respectively.

Fabrication tolerance study
The fabrication tolerance of the suggested V-shaped PCF sensor is also studied to ensure the robustness of the suggested design to fabrication imperfection. In this study, fabrication tolerance of ± 5% is tested (Chang et al., 2019). It is worth mentioning that, only one parameter is studied while the other parameters are fixed at their optimum values. Table 5 (5) R = Δn a Δλ min Δλ r (RIU) Fig. 9 Linear relation between the RI of the analyte and resonance wavelength for quasi TM and quasi TE mode respectively shows the tolerance analysis of the thickness of the ZrN layer (t), radius of the air holes (r), and distance between the two V-shape vertices (x) of the proposed design. It is evident that the studied parameters have a good tolerance of ± 5% where the sensitivity is still better than 5800 nm/RIU. Therefore, the proposed sensor is feasible for fabrication with good fabrication tolerance. A comparison between the proposed V-shaped cancer detection sensor with the previously reported sensors in (Sani et al. 2022), (Verma, Kumar and Jindal, 2022), (Chaudhary, Kumar and Kumar, 2022), (Rajeswari and Revathi 2022), (Yasli 2021), (Jabin et al. 2019), (Ayyanar et al. 2018), (Mollah et al. 2020), (Ramanujam et al. 2019), (Jindal et al. 2016), (Abdelghaffar et al. 2022) and (Ramanujam et al. 2019), (Singh and Prajapati 2023), (Almawgani et al. 2022), (Malek et al. 2022), (Yasli 2022), (Nayak 2023) is depicted in Table 6. It is evident that the sensitivity of the proposed early cancer detection biosensor is greater than the introduced biosensors in (Jabin et al. 2019), (Ayyanar et al. 2018), (Ramanujam et al. 2019), (Jindal et al. 2016), (Sani et al. 2022), (Almawgani et al. 2022), (Malek et al. 2022), (Yasli 2022) (Nayak 2023) (Abdelghaffar et al. 2022)and (Ramanujam et al. 2019). Further, the highly sensitive sensors reported in (Verma, Kumar and Jindal, 2022), (Yasli 2021), and (Singh and Prajapati 2023) have used different air hole diameters with complex fabrication process. Further, (Verma, Kumar and Jindal, 2022) and (Singh and Prajapati 2023) have used dual plasmonic materials with higher fabrication cost. In Addition, (Mollah et al. 2020) and (Rajeswari and Revathi 2022) have used internal coating for the plasmonic material through the PCF which is a challenge for fabrication. Further, analyte infiltration will be difficult through the PCF air holes as reported in (Mollah et al. 2020). Moreover, the plasmonic PCF biosensors reported in (Jabin et al. 2019), (Ayyanar et al. 2018), (Mollah et al. 2020), (Ramanujam et al. 2019), (Jindal et al. 2016), (Abdelghaffar et al. 2022) and (Ramanujam et al. 2019) were used to sense either breast or blood cells only, while the suggested biosensor offers early cancer detection for breast, basal and cervical cells.
Surface plasmon sensors have high sensitivity compared to traditional optical biosensing techniques (Azab, Hameed and Obayya, 2023). In addition, surface plasmons based techniques don't need an expert to run the test. However, most of traditional optical sensing techniques can diagnose the cancer at late stages, while surface plasmons can detect cancer in early stages (Azab, Hameed and Obayya, 2023). A comparison between surface plasmons and the colorimetric method and the optical coherence tomography as traditional techniques is introduced in Table 7. It is worth noting from this table that the colorimetric method is the lowest in cost with an average cost of USD ~ 800, while it requires an expert for operation, and high error degrees may arise (González-González et al., 2021). Further, the optical coherence tomography suffers from the highest cost around USD ~ 7164 (Chopra, Wagner and Keane, 2021). On the other hand, surface plasmons have an average cost of USD ~ 6000 (Azab, Hameed and Obayya, 2023).

Conclusion
A novel V-shaped highly sensitive label-free PCF biosensor based on alternative plasmonic materials is introduced and studied, using FVFEM. The suggested design can be used for breast, basal, cervical cancer detection with sensitivities of 6214.28 nm/RIU, 3800 nm/ RIU, and 5008.33 nm/RIU, and resolution of 2.728 × 10 −5 RIU,2.63 × 10 −5 RIU , and 1.92 × 10 −5 RIU , respectively, for the quasi TM core mode. Further, it offers sensitivities of 6000 nm/RIU, 4400 nm/RIU, and 5333.3 nm/RIU, and resolution of 1.666 × 10 −5 RIU ,2.27 × 10 −5 RIU , and 1.875 × 10 −5 , respectively, for quasi the TE mode. Furthermore, the proposed design has high linearity for the quasi TM and quasi TE modes. The biosensor could be easily fabricated by current PCF fabrication technologies with high fabrication tolerance. Overall, the suggested design is qualified for biological and chemical analyte RI sensing with high sensitivity.
Author contributions MFOH has proposed the idea. MA and YG have done the simulations of the reported sensor. All authors have contributed in the analysis, discussion, writing and revision of the paper. MFOH and SSAO have supervised the work done through the submitted manuscript.
Funding Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB). The authors acknowledge the financial support from the Science, Technology & Innovation Funding Authority (STIFA) in Egypt under the project (ID: 45702).

Data availability
The data will be available upon request.

Conflict of interest
The authors would like to clarify that there is no financial/non-financial interests that are directly or indirectly related to the work submitted for publication.

Ethical approval
The authors declare that there are no conflicts of interest related to this article.
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