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Comprehensive 16S rDNA Sequencing and LC–MS/MS-Based Metabolomics to Investigate Intestinal Flora and Metabolic Profiles of the Serum, Hypothalamus and Hippocampus in p‑Chlorophenylalanine-Induced Insomnia Rats Treated with Lilium brownie

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Abstract

Gut microbiota homeostasis in the organism and insomnia have been reported to influence each other. In the study, a method of 16S rRNA gene sequencing combined with ultra-high performance liquid chromatography-mass/mass spectrometry was adopted to evaluate the effects of Lilium brownie (LB) on intestinal flora and metabolic profiles of serum, hypothalamus and hippocampus in insomnia rat induced by p‑chlorophenylalanine (PCPA). It was observed that the imbalance in the diversity and abundance of gut microbiota induced by PCPA was restored after LB intervention. Among these, the Porphyromonadaceae, Lactobacillus and Escherichia were significantly adjusted at the genus level by PCPA and LB, respectively. It was also found that the most of metabolic phenotypes in serum, hypothalamus and hippocampus perturbed by PCPA were regulated towards normal after LB intervention, especially 5-hydroxy-l-tryptophan of the hypothalamus involving in 5-HT metabolism. Moreover, the arachidonic acid metabolism in serum, hypothalamus and hippocampus, and the serotonergic synapse in hypothalamus and hippocampus were the most fundamentally and significantly affected pathways after LB intervention. The results of correlation analysis showed that several floras including Pseudoruegeria have an outstanding contribution to the change of differential metabolites. In brief, the results confirm that gut microbiota is significantly returned to normal and may interact with the corresponding metabolites to relieve insomnia under LB intervention.

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Funding

This research was funded by financial support from the Chinese Postdoctoral Science Foundation (2017M623342).

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Authors and Affiliations

  1. Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China

    Yanpo Si, Tao Guo, Wenjun Wei, Lili Wang, Fei Zhang, Xiaoya Sun & Mengqi Liu

  2. School of Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou, 450046, China

    Xiaohui Chen

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  1. Yanpo Si
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Si, Y., Chen, X., Guo, T. et al. Comprehensive 16S rDNA Sequencing and LC–MS/MS-Based Metabolomics to Investigate Intestinal Flora and Metabolic Profiles of the Serum, Hypothalamus and Hippocampus in p‑Chlorophenylalanine-Induced Insomnia Rats Treated with Lilium brownie. Neurochem Res 47, 574–589 (2022). https://doi.org/10.1007/s11064-021-03466-z

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