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Construction of mannose-grafting Fe-doped carbon dots for CpG delivery and photothermal ablation of tumor

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Abstract

Photothermal therapy (PTT) can kill the tumor in situ, but fail to trigger strong antitumor immune response against its recurrence and metastasis. Herein, mannose-grafting Fe-doped carbon dots (M/Fe-CDs) were prepared via hydrothermal carbonization and chemical modification. The prepared M/Fe-CDs had characteristics of excellent optical qualities, good water solubility, and outstanding biocompatibility. Compared with pure CDs, the doping of Fe atom endowed the M/Fe-CDs with a high photothermal conversion efficiency (from 12.1 up to 30.1%). Under 808-nm NIR irradiation, the M/Fe-CDs could lead to local hyperthermia to kill tumor cells. Importantly, the M/Fe-CDs were capable of loading CpG-ODNs via electrostatic absorption and delivering them into the cytoplasm of dendritic cells (DCs). Finally, the CpG-ODNs-loading M/Fe-CDs could significantly increase the CD80/86 expression on the DCs surface. These results demonstrated that the M/Fe-CDs could be used as a versatile nanoplatform to combine the PTT and immune activation for tumor treatment.

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The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Funding

This work was financially supported by the Zhenjiang Key Research and Development Program-Social Development (SH2021030, SH2022100), the Young Talent Development Plan of Changzhou Health Commission (CZQM 2022018), and the Baoshan District Medical Health Project (20-E-11).

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  1. Haiyang Ye, Li Zhang and Xiaonan Qiu contributed equally to this work.

Authors and Affiliations

  1. Clinical Laboratory, The Third People’s Hospital of Changzhou, Changzhou, 213001, People’s Republic of China

    Haiyang Ye, Guojun Zheng & Yunchao Wu

  2. Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Haiyang Ye, Xiaonan Qiu, Jianghong Qiu, Wenqing Xu, Xiao Xu, Huijun Yu, Shengqiang Zou & Fengyi Du

  3. Department of Critical Care Medicine Unit, Shanghai Baoshan District Wusong Central Hospital, Shanghai, 201900, People’s Republic of China

    Li Zhang

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  1. Haiyang Ye
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  2. Li Zhang
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Contributions

Haiyang Ye, Xiaonan Qiu, Li Zhang, and Jianghong Qiu are responsive for experimental operation. Yunchao Wu, Wenqing Xu, and Xiao Xu are responsive for data collection and analysis. Huijun Yu, Shengqiang Zou, Guojun Zheng are responsive for data processing and drawing. Fengyi Du is responsive for experimental design and writing.

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Correspondence to Yunchao Wu.

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Ye, H., Zhang, L., Qiu, X. et al. Construction of mannose-grafting Fe-doped carbon dots for CpG delivery and photothermal ablation of tumor. J Nanopart Res 26, 25 (2024). https://doi.org/10.1007/s11051-024-05936-1

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