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A Reliable Murine Model of Disseminated Infection Induced by Talaromyces Marneffei

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Abstract

Talaromycosis (penicilliosis) caused by Talaromyces marneffei is one of the most important opportunistic infection diseases in tropical countries of South and Southeast Asia. Most infections occurred in individuals with human immunodeficiency virus (HIV) and the primarily reason for the increase in the number of the cases is HIV pandemic. The pathogenesis of T. marneffei infection is unclear. There is still no ideal animal model for studying talaromycosis. In this study, we developed a stable, safe and maneuverable murine model that mimics human T. marneffei disseminated infection using T. marneffei yeast intraperitoneal injected to BALB/c nude mice. We successfully observed symptoms similar to those seen in clinical patients in this murine model, including skin lesions, hepatosplenomegaly, pulmonary infection and mesenteric lesions. We further studied the pathological changes of various tissues and organs in the infected animals to help better understand the severity of the infection. This model may provide a good tool for studying disseminated infection induced by T. marneffei.

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Funding

This research was funded by grants from Project for AIDS Bureau of Yunnan Province; “Ten-thousand Talents Program" of Yunnan Province (YNWR-MY-2018–039); Natural Science Foundation of China (81560325, 81660737); Yunnan Province Clinical Research Center for Skin Immune Diseases(2019ZF012);Yunnan Province Clinical Center for Skin Immune Diseases (ZX2019-03–02);Innovation Team of Department of Science and Technology of Yunnan Province (2018HC005).

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  1. Juan He and Jia-Sheng Li have contributed equally to this work.

Authors and Affiliations

  1. Department of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan, China

    Juan He, Hong-Yan Xu, Hong-Bin Li, Zhe Li, Hui-Ling Zhou & Yu-Ye Li

  2. College of Chinese Materia Medica, Yunnan University of Chinese Medicine, 1076 Yuhua Road, Chenggong, Kunming, 650500, Yunnan, China

    Jia-Sheng Li, Jun Li & Rui-Rui Wang

  3. NHC Key Laboratory of Drug Addiction Medicine, First Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, 650032, China

    Yi-Qun Kuang

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  1. Juan He
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Contributions

Conceived and designed the experiments: RW YL. Performed the experiments: JH JL HX JL ZL HZ. Analyzed the data: JH YK HL. Contributed reagents/materials/analysis tools: HL RW YL. Contributed to the writing of themanuscript: JH RW YL.

Corresponding authors

Correspondence to Rui-Rui Wang or Yu-Ye Li.

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The authors declare that they have no conflict of interest.

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The authors affirm that human research participants provided informed consent for publication of the images in Fig. 2I, 2J. Patients signed informed consent regarding publishing their data and photographs.

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Handling Editor Cunwei Cao.

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He, J., Li, JS., Xu, HY. et al. A Reliable Murine Model of Disseminated Infection Induced by Talaromyces Marneffei. Mycopathologia 187, 53–64 (2022). https://doi.org/10.1007/s11046-021-00596-4

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  • DOI: https://doi.org/10.1007/s11046-021-00596-4

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