Investigations on speaker adaptation using a continuous vocoder within recurrent neural network based text-to-speech synthesis

This paper presents an investigation of speaker adaptation using a continuous vocoder for parametric text-to-speech (TTS) synthesis. In purposes that demand low computational complexity, conventional vocoder-based statistical parametric speech synthesis can be preferable. While capable of remarkable naturalness, recent neural vocoders nonetheless fall short of the criteria for real-time synthesis. We investigate our former continuous vocoder, in which the excitation is characterized employing two one-dimensional parameters: Maximum Voiced Frequency and continuous fundamental frequency (F0). We show that an average voice can be trained for deep neural network-based TTS utilizing data from nine English speakers. We did speaker adaptation experiments for each target speaker with 400 utterances (approximately 14 minutes). We showed an apparent enhancement in the quality and naturalness of synthesized speech compared to our previous work by utilizing the recurrent neural network topologies. According to the objective studies (Mel-Cepstral Distortion and F0 correlation), the quality of speaker adaptation using Continuous Vocoder-based DNN-TTS is slightly better than the WORLD Vocoder-based baseline. The subjective MUSHRA-like test results also showed that our speaker adaptation technique is almost as natural as the WORLD vocoder using Gated Recurrent Unit and Long Short Term Memory networks. The proposed vocoder, being capable of real-time synthesis, can be used for applications which need fast synthesis speed.


Introduction
Text-to-speech (TTS) synthesis, a field of speech processing, converts the written text into speech. It is used in many application and industrial fields such as smartphones, railway announcements, robotics, healthcare, and education [17,37]. It offers many benefits for students with a learning disability [5]. It can be used to overcome time consumed by reading via audiobooks using a TTS. It can also enhance literacy skills, improve reading comprehension, the ability to recall information, and the pronunciation improvement [38,40]. It also makes it easy for anyone else searching for more convenient access to digital materials. TTS health-assistance technologies would significantly enhance people's health and lower their healthcare expenditures [22,23].
One type of text-to-speech synthesis employs statistical parametric synthesis (SPSS), with two primary machine learning techniques: deep neural networks (DNN) and Hidden Markov models (HMM). DNNs have become the prevalent acoustic models for text-tospeech synthesis. It is used to generate high-level data representations.
Furthermore, a considerable improvement in voice quality may be accomplished with the availability of multi-task learning [45,49]. In the parametric model, the speech signal is processed into parameters indicating fundamental frequency (F0) and speech spectrum, subsequently, input into a machine learning system. After the statistical model has been learned on the training data, the parameter sequences are rebuilt into speech signals utilizing reconstruction techniques (excitation models, vocoders).
SPSS naturalness can be improved through further developing vocoding approaches. Although neural vocoding systems can produce speech similar to natural speech (e.g., WaveNet [41]), and WaveGlow [32], their high computational complexity remains problematic. Additionally, they necessitate vast data and processing resources, making it challenging to train for real-time applications, particularly in embedded environments. SPSS, which uses vocoders to model high-quality synthetic speech, is an efficient and more easily adaptable solution. Besides, controllability options make vocoder-based SPSS preferable over neural vocoders.
Deep learning algorithms perform exceptionally well when training on a large enough dataset. Subsequently, this technique means that to train a model for speech synthesis, we need a lot of speech from a target speaker (e.g., 60 minutes). Eventually, it limits the technology's capacity to scale to a variety of voices. In TTS, the simple fine-tuning creates a high-quality model when the data from the target speaker is adequate [20]. In this case, we can restrict the tuning to a particular part alternately to the whole system to avoid overfitting [9,26] Adaptation methods may be used to produce new voices using only a small amount of adaptation data from a target speaker [27]. Because of the potential performance of speaker adaptation, SPSS has a significant advantage over unit-selection speech synthesis in terms of changing speaker attributes, emotions, and speaking styles [25]. Recent neural vocoders can produce incredibly natural speech, yet they usually fall short of real-time synthesis requirements [30,34].
We proposed in our previous work [28] a speaker adaptation system based on a continuous vocoder and a Feed-forward deep neural network (FFNN) text-to-speech synthesis system. In this paper, we investigated the recurrent neural networks (RNNs) such as Long Short Term Memory networks (LSTM) and Gated Recurrent Unit (GRU) in both WORLD vocoder (baseline system) and continuous vocoder. As well, on the basis of data from nine speakers, we show that an average voice can be trained for DNN-TTS and that speaker adaptability is possible with 400 utterances (about 14 minutes).
The remaining part of the paper is structured as follows. Section 2 summarizes the speech signal structure. Section 3 comprises a summary of relevant scientific publications, including innovative approaches in text-to-speech synthesis based on speaker adaptation. In Section 4, the continuous vocoder is introduced in detail and compared to other vocoders. The design of the system, tools, and dataset are described in Section 5. The results are then explained in (Section 6). Finally, in Section 7, the conclusion is given.

Speech signal structure
A concept is initially generated in the speaker's brain at the linguistic level of communication before being translated into words, phrases, and sentences. The brain makes electrical signals that move at the nerves at the physiological level. The vocal cords and tract muscles are triggered by these electrical signals. Changes in vocal tract pressure are caused by the movement of the vocal cords and vocal tract, and the lips produce the sounds [33].
The vibration of vocal cords creates the voiced sounds, and the rate of vibration of vocal cords is referred to as a fundamental frequency (F0). The perceptual correlate of F0 is named pitch. The formant frequencies generally are the energy attention at higher frequency ranges.
In the time domain, the speech waveform represents time on the linear horizontal axis and amplitude on the vertical axis. The primary levels of timing information in the speech acoustic are envelope, periodicity, and fine structure (the top part of Fig. 1). The envelope shows the slow outline of the overall changes in the intensity over the signal. The repeating peaks at a regular periodic rate mean we have voiced sounds. The fine structure possesses all high frequencies ups and downs in the waveform corresponding to the highest frequency changes. In the frequency spectrum, the frequency is on the horizontal axis and the amplitude on the vertical axis. Finally, the spectrogram shows the frequency content change over time the bottom part of (Fig. 1).

Related work
In addition to improving the naturalness of synthesized speech, synthesizing new voices with a small amount of data is an enthusiastic research topic. Numerous approaches are proposed to address this concern. They all share the same basic principle: use a large corpus to repay a target speaker's lack of data. Because DNNs have a high number of parameters and the parameters of the DNN model cannot be interpreted as directly as the parameters of the HMM model, DNN-based TTS adaptation is more complicated than HMM-based adaptation [7].
One way for speaker adaptation is to transmit speaker codes to the main network, which can then adjust the layer weights. These speaker codes can be learned or i-vectors. A supervised method of learning speaker codes for DNN-HMM systems' speaker adaptation was suggested by [48]. These algorithms learn a bias for the sigmoid nonlinearities at the product of fully connected layers employing speaker codes. Besides, a neural fusion architecture of a unit concatenation approach and SPSS to enhance the similarity of the synthesized speech to the target speaker was proposed [11]. Despite that, the naturalness has not been upgraded.
In addition, auxiliary speaker embedding-based DNN adaptation strategies encode speaker-dependent (SD) properties in a compact vector representation in additional speaker embedding-based approaches. SD embedding vectors are employed as supplemental input Fig. 1 The speech waveform and spectrogram info. of the word "nudge" (drawn using Praat software) features for DNNs, allowing for more effortless speaker adaptability during system training and evaluation. The SD auxiliary feature estimation is done separately from the other components of the recognition system. An instance of i-vectors were learned from universal background models based on the Gaussian mixture model was given by [36]. D-vectors have been employed to transmit the speaker-specific data in adaptation [6]. A DNN speaker adaptation framework based on full Bayesian learning to simulate speaker-dependent (SD) parameter uncertainty with limited speaker-specific adaptation data was offered by [47].
Some speaker adaptation approaches are model-based adaptation techniques (e.g., the learning hidden unit contributions (LHUC)). The amount of speaker-specific adaptation data effectively influences the modeling subdivisions of the SD parameters in this design. When trained on a large quantity of speech data with multiple speakers, the LHUC technique posits that a neural network's hidden representations are base vectors learned to interpret the acoustic space of several speakers [44].
Zero-shot multi-speaker TTS (ZS-TTS) employs only a few seconds of speech dataset to create synthesized voices for target speakers. SC-GlowTTS is a novel model (GlowTTS and HiFi-GAN) to enhance the likeness to the target speaker using ZS-TTS [8]. The all pass warp (APW) has been integrated into neural network frameworks and investigated in ZS-TTS [35]. Their research shows that APW improves multi-speaker model generalizability and speaker similarity to the target speaker. Better speaker similarity is achieved by a multi-model speaker adaptation system (an unsupervised speech representation module and Tacotron2) than by a single-model (Tacotron2) [50]. In addition, intriguing research develops a multi-model speaker adaptation, the Hieratron model framework, to control the prosody and timbre and synthesize the speech from a limited dataset [14].
Further, the few-shot adaptation technique modifies the network's parameters with the target speaker's speech. Employing two special encoders (fine-grained and coarse-grained) for cloning unseen target speakers with a few data samples has increased the similarity and quality of synthesized speech [12]. AdaSpeech 3 is an adaptive TTS model fine-tuning a trained reading style TTS model for spontaneous speech [24]. Using a small amount of target speech data, the model predicts filled pauses and changes in rhythm.
Hybrid deep learning (HDL) may represent the future direction of deep learning. It combines many artificial neural network concepts such as convolutional neural network (CNN), RNN, and Autocoder in one model. We previously tested our Continuous vocoder to build a deep learning model based on TTS by applying a Hybrid model of Bi-LSTM and conventional Uni-RNN [4]. In future research, we believe it is worth investigating the speaker adaptation with the Continuous vocoder using a hybrid model.
The fine-tuning approach belongs to the area of transfer learning, in which information gained from one activity may be applied to another. It was used in speech synthesis to train an average voice model (AVM) on a large database of speakers before applying the same network to a new speaker with fewer data. This technique was found to exceed the synthesized speech of LHUC and an HMM-based adaptation method [39]. Due to the lack of conducted research in speaker adaptation using the fine-tuning approach based-TTS, we used in the current study a novel technique utilization low complexity of continuous vocoder with a small database of target speakers to synthesize speech.
The vocoder is an essential aspect of TTS. It is responsible for translating human voices to vocoder parameters and vice versa. It is both an analyzer and a synthesizer. It analyzes speech by converting the waveform into a set of parameters that reflect the excitation signal of the vocal folds and then modifying the excitation signal with a vocal-tract filter transfer tool. Oppositely, it uses the parameters to reconstruct the original voice stream. The parametric representation allows for statistical modeling of speech as well as manipulation of speech to improve intelligibility. STRAIGHT, WORLD, Mel -generalised cepstral vocoder, adaptive harmonic model, Glottal vocoder, and Harmonic model are a few examples of vocoders [18].

Continuous vocoder
During the analysis stage of the Continuous vocoder [13], the fundamental frequency (contF0), is determined on the input waveforms. The maximum voiced frequency (MVF) parameter is then calculated from the speech signal. As the next step, the voice signal is subjected to 24-order Mel-Generalized Cepstral analysis (MGC) using gamma = −1/3 and alpha = 0.42. In all phases of the analysis, the frameshift is 5 ms. The Glottal Closure Instant (GCI) algorithm is used to find the glottal period restrictions of particular periods in the voiced parts of the inverse filtered residual (see Fig. 2).
After that in the synthesis stage, depending on the contF0, voiced excitation is made up of principal component analysis (PCA) residuals that overlap-added pitch simultaneously. Subsequently, at the frequency specified by the MVF parameter, this voiced excitation is low-pass filtered frame by frame. White noise is employed at frequencies greater than MVF's value. Then, both voiced and unvoiced excitation are combined. Applying a time domain envelope to the unvoiced segments is presented as a means of modeling unvoiced sounds to eliminate any remaining buzziness and reduce the noise part [1,3]. Consequently, to synthesize speech from the excitation and MGC parameter stream, a Mel generalized-log spectrum approximation (MGLSA) filter is utilized. The continuous vocoder is adaptable and straightforward, with only two 1-dimensional parameters for modeling excitation. It can implement its synthesis algorithm in real-time because it is computationally viable. It has the convenience of not requiring a voiced/unvoiced decision, which implies that alignment failures between voiced and unvoiced portions are shunned. But on the other hand, failure in MVF modeling can result in voicing issues. The continuous vocoder is also viable in statistical voice conversion utilizing a feed-forward deep neural network and is comparable to the baseline WORLD vocoder [2].
Furthermore, utilizing a temporal envelope such as Discrete All Pole, Frequency Domain Linear Prediction, Low Pass Filter, and True envelopes, the continuous vocoder accurately simulates the noise component of the excitation source [4]. This feature offers our continuous vocoder the advantage over the most generally used vocoder STRAIGHT [21] because the noise segment of the excitation reference is not precisely modeled in the excitation parameters of STRAIGHT [15].

Baseline: WORLD vocoder
We chose the WORLD vocoder as a baseline due to its ability to synthesize high-quality voice [29]. The WORLD vocoder employs a source-filter-based speech production model with mixed excitation, alike to the legacy STRAIGHT vocoder [43]. The WORLD vocoder uses DIO, CheapTrick, and PLATINUM algorithms to extract the parameters (F0, spectral envelope, and aperiodic parameter), respectively. The above continuous vocoder uses two one-dimensional parameters to generate the excitation signal, whereas the WORLD system employs a five-dimensional band aperiodicity. Moreover, unlike the continuous vocoder, the WORLD vocoder might carry errors at transition boundaries of voiced and unvoiced parts of speech [4].

Methodology
For both systems using the continuous and WORLD vocoders, we performed the methods outlined below. We created an average voice model (AVM), which we then customized for the target speaker. The open-source Merlin [46] framework was utilized to apply the generic FFNN, and RNN model-based TTS.

Speech corpus
The research work was carried out using the VCTK-Corpus [42]. This CSTR VCTK Corpus includes voice data from 109 English speakers (62 female) who speak diverse accents. A total of 400 phrases are read by each speaker. The sentences were taken from a newspaper, the rainbow passage, and an elicitation section. The dataset is around 44 hours long in total. All of the recordings were made at a sampling rate of 96 kHz (24 bits) and subsequently down-sampled to 48 kHz. The dataset was created for multi-speaker TTS and speaker-adaptive purposes [10,31]. We re-sampled the waveforms of the database to 16 kHz. The training experiment employed 85 percent of the data, while the testing and validation experiments used the remaining data.

Build an average voice model
This strategy is classified as transfer learning, which means that the learnings in one activity can be applied to another similar work. In speech synthesis, an AVM can be trained on a large sample of speakers and then used to adjust to a new speaker with fewer data. The AVM learns how the text and speech features are related. It can change some of the parameters (i.e., weights of the neural networks) for the new speaker. We developed the AVM throughout the range of 9 speakers (six females (p225, p228, p229, p230, p231, and p233) and three males (p226, p227, and p232). We have created the data and directories, labels, acoustic characteristics (MGC, MVF, and lf0) for the continuous and (lf0, mgc, and bap) for the WORLD vocoder. Then we trained the acoustic and duration models. We trained these models using FFNN, LSTM, and GRU.

Adapt the AVM for the target speakers
To overcome limited data of the target speaker, this model collects universal features from the average voice (multiple speakers) model and is trained independently for the target speaker. For analysing purposes, we did adaptation experiments with four speakers (p234 (female), p236 (female), p237 (male), and p247 (male)). We further trained the average acoustic and duration models for the four target speakers by using FFNN, LSTM, and GRU. Each speaker's adaptation data consists of 400 utterances (about 14 minutes). Finally, we synthesized the sentences using the Continuous vocoders and compared the resulted synthesized speech with the results that we obtained by the baseline vocoder.

Training topology
Feed-forward neural networks and a couple of versions of the recurrent neural networks (LSTM and GRU) utilized in our study are described in this part. These neural networks are used to predict speech reconstruction acoustic features, and textual and phonetic transcriptions are turned into a sequence of linguistic features as input. Hyperparameters are the same for both systems (Continuous and WORLD vocoders). We engaged a high-performance NVidia Titan X graphics processing unit (GPU) for the networks' training.

Feed-Forward Neural Networks
FFNN is the simplest type of neural network that can be used for TTS purposes. We applied the FFNN of six hidden layers with tangent hyperbilic units (1024 neurons, sgd optimizer, 25 epochs, and 265 batch size).

Long Short Term Memory networks
Unlike feed-forward mapping, which is done frame by frame of speech, linguistic attributes are sequence-to-sequence mapped to vocoder parameters in RNN [16].
At the bottom, we used a four-layer feed-forward neural network. We utilized LSTM on top of the feed-forward layers. The feed-forward layers have 1024 hidden units using a tangent activation function in each layer.
Meantime, LSTM implementations used 512 units. In addition, Adam optimizer was used. The hyperparameters were set up, including batch size 512, dropout rate 0.1, learning rate 0.001, and training epochs 30.

Gated Recurrent Unit
GRUs are incredibly similar in architecture to LSTM, where both use gates to govern data flow in networks. The differences in their architecture are the GRU does not have a cell state, and LSTM has three gates (input, forget, and output) while GRU has only reset and update gates.
We used a four-layer feed-forward at the bottom of the neural network design. After that, we utilized GRU on top of the feed-forward layers. The feed-forward layers have 512 hidden units using a tangent activation function in each layer. In addition, GRU executions used 256 units. Moreover, Adam optimizer was used. The hyperparameters were set up, including batch size 256, dropout rate 0.0, learning rate 0.002, and training epochs 30.
The pseudocode of our work is summarized in the following steps:

Results
We used objective and subjective evaluations to test the quality of the synthesized waves of the target speaker. We compared all the results obtained by continuous vocoder with WORLD vocoder. In the objective evaluation, we independently tested our continuous vocoder parameters with speaker adaptation in deep neural networks using Mel-Cepstral Distortion (MCD), F0-correlation (F0-CORR), and spectrogram analysis.

MCD(dB):
We determined the Mel-Cepstral Distortion measure coefficients per the whole model output (1). The MCD is a measure of the difference between two mel cepstra sequences. The notion is that the lower the MCD between synthesized and natural mel cepstral sequences, the closer synthetic speech is to natural speech reproduction. Based on these findings, the efficiency of the continuous vocoder systems against the WORLD baseline scheme could be concluded (Table 1). (1) x and y are the i th cepstral coefficients of the natural and synthetic speech signals, respectively. Table 1 shows slightly better MCD values of WORLD vocoder with FFNN than continuous vocoder FFNN. In contrast, with LSTM and GRU, the continuous vocoder was slightly better compared to the WORLD vocoder, while being significantly not different. Also, we can observe some speaker dependency, i.e., for two out of the four speakers, the WORLD has lower MCD, while for the other two speakers, the Continuous vocoder has lower MDC values. Thus, we can say that the two vocoders are roughly equivalent in this measure.

F0-CORR:
The similarity between reference and synthesized data is reflected in the correlation (whether they are linearly related or not). Overall, a measurement is taken frame per frame (2). F0-CORR shows promising results for continuous vocoder as the values are closer to 1 and higher than the baseline WORLD vocoder (Table 2). This difference in obtained results might be related to the discontinuous fundamental frequency model of the WORLD vocoder. In terms of F0 correlation, we can observe speaker-dependent differences: in the case of FFNN and LSTM, the continuous vocoder was preferred by three speakers out of four, while with the GRU system, the continuous vocoder was  preferred by all four speakers. Thus, we can conclude that the continuous vocoder was ranked as being better than the WORLD baseline in terms of F0 modeling. This can be explained by the way they model F0 (see Section 3).
Where x i is the natural speech frame, y i is the synthesized speech frame, x is the mean of the natural speech frame, and y is the mean of the synthesized speech frame. 3. Demonstration sample (spectrogram): Figure 3 shows the spectrograms of synthesized sounds by both vocoders (WORLD and continuous). We present six spectrogram plots of three speakers of two females (p237, p236) and a male (p247) reading the text "People look, but no one ever finds it.". As can be seen in the spectrograms in the right column, the continuous vocoder distinguishes between voiced and unvoiced speech frequencies based on the MVF parameter (i.e., spectral content below MVF is voiced, while spectral range above MVF is unvoiced). The spectrograms in the left column do not show such a strong separation curve in terms of frequency, because the WORLD vocoder handles voicing via band aperiodicities. Thus, the main difference between the baseline WORLD and the proposed continuous vocoder is in the way they model the excitation component of speech.

Subjective evaluation
We ran an online MUSHRA-like test to analyze TTS variants [19]. This investigation compared the synthesized phrases generated by the baseline (WORLD) and the proposed (Continuous) vocoders. Natural versions of the sentences were included as references. Besides, we created a lower anchor variant, a spectrally distorted version of the natural Fig. 3 The spectrogram plot of six synthesized sounds (a) a synthesized female voice (p237) using WORLD vocoder with FFNN, (b) a synthesized female voice (p237) using continuous vocoder with FFNN, (c) a synthesized male voice (p247) using WORLD vocoder with LSTM, (d) a synthesized male voice (p247) using continuous vocoder with LSTM, (e) a synthesized female voice (p236) using WORLD vocoder with GRU, and (f) a synthesized female voice (p236) using continuous vocoder with GRU sentence. Subjects were requested to score the naturalness of each stimulus on a scale of 0 (very unnatural) to 100 (fully natural or realistic). We generated 19 sentences from the VCTK-Corpus using the four target speakers, of which 12 were selected for the test. The samples were displayed in a random order (different for each participant). The MUSHRA test comprised 96 utterances (8 systems x 4 speakers x 12 sentences).
Audiences were provided an example to listen to before the test to adjust the loudness. Twenty one non-native English speakers (17 silent situations, 4 in a noisy environment; one female, 20 males; 26-41 years old) scored each sentence. The test took an average of 18 minutes to achieve. Figure 4 displays the average naturalness scores for the approaches that were tried. Original utterances received 87 out of 100 scores from the listeners. The synthesized speech using WORLD vocoder with FFNN obtained higher scores (52 out of 100) than our model using the same network topology (39). On the other hand, both systems with GRU received almost the same votes (57 and 50, respectively). The continuous vocoder showed an almost equivalent performance (53 out of 100) to the WORLD vocoder (59) by using LSTM.  Figure 5 shows the MUSHRA test results speaker by speaker. For all speakers, the WORLD vocoder with FFNN was preferred over the continuous vocoder. GRU presents a different scenario. Both systems are almost equivalent in the higher f234, f236, and m247. With the speaker m237, the WORLD vocoder obtained more evaluation scores than our system. Moreover, WORLD vocoder obtained more scores with a synthesized speech by the three speakers (f234, f237, and m247) than continuous vocoder using LSTM except for one speaker (f236), which shows equal results. These disparities are statistically significant (Mann-Whitney-Wilcoxon ranksum test, with a 95% confidence level). Despite the fact that the naturalness scores of the Continuous vocoder did not match those of the WORLD vocoder, we may infer that the speaker adaptation experiment with the suggested vocoder was a success and we obtained reasonable results.

Conclusions and future work
Recent neural vocoders, while being capable of synthesizing highly natural speech, often also fall short of the criteria of real-time synthesis. Classical vocoder-based SPSS can be effective in applications demanding modest computer complexity (e.g., a chatbot in a highload environment like a government website, or a low-resource smartphone). This research aimed to present a new strategy for improving the accuracy of our prior continuous vocoder with speaker adaptation. We employed the continuous vocoder, in which the excitation is characterized with two one-dimensional parameters: continuous F0 and MVF. During our evaluation experiments, we prove that using data from multiple speakers it is achievable to train an average voice model and that speaker adaptation is possible with just 400 utterances (roughly 14 minutes). The performance strengths and shortcomings of each of the proposed approaches (FFNN, GRU, and LSTM) for four different speakers were analyzed using a number of metrics. The objective testing revealed that our vocoder is capable of synthesizing voices using RNN topologies roughly equivalent to the baseline WORLD vocoder. Furthermore, the MUSHRA test results proved the efficacy of our speaker adaptation strategy, being only slightly worse than the WORLD vocoder in the case of GRU and LSTM. The WORLD vocoder obtained significantly higher scores than our system in the case of FFNN. Text-to-speech synthesis with a low-complexity vocoder and speaker adaptation can help more natural human-computer interaction.
In future research, we will explore the speaker adaptation with the Continuous vocoder using a hybrid model (e.g., RNN, CNN, gcForest, etc.). These models deliver more effectively than the individual deep neural networks. Moreover, Transformer neural networks, a state-of-the-art technique in natural language processing (NLP), with our model is our target for the next search step. In addition, building a multi-model speaker adaptation system of the Continuous vocoder with state-of-the-art sequence-to-sequence models such as Tacotron2, FastPitch, etc., more advanced solutions would be considered.

Conflict of Interests
The authors have no relevant financial interests in the manuscript and no other potential conflicts of interest.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory