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Schizandrin A attenuates early brain injury following subarachnoid hemorrhage through suppressing neuroinflammation

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Abstract

Background

Early brain injury (EBI) is the vital factor in determining the outcome of subarachnoid hemorrhage (SAH). Schizandrin A (Sch A), the bioactive ingredient extracted from Schisandra chinensis, has been proved to exert beneficial effects in multiple human diseases. However, the effect of Sch A on SAH remains unknown. The current study was designed to explored role and mechanism of Sch A in the pathophysiological process of EBI following SAH.

Method

A total of 74 male C57BL/6 J mice were subjected to endovascular perforation to establish the SAH model. Different dosages of Sch A were administrated post-modeling. The post-modeling assessments included neurological test, brain water content, RT-PCR, immunofluorescence, Nissl staining. Oxygenated hemoglobin was introduced into microglia to establish a SAH model in vitro.

Result

Sch A significantly alleviated SAH-induced brain edema and neurological impairment. Moreover, application of Sch A remarkably inhibited SAH-induced neuroinflammation, evidenced by the decreased microglial activation and downregulated TNF-α, IL-1β and IL-6 and expression. Additionally, Sch A, both in vivo and in vitro, protected neurons against SAH-induced inflammatory injury. Mechanismly, administration of Sch A inhibited miR-155/NF-κB axis and attenuated neuroinflammation, as well as alleviating neuronal injury.

Conclusion

Our data suggested that Sch A could attenuated EBI following SAH via modulating neuroinflammation. The anti-inflammatory effect was exerted, at least partly through the miR-155/NF-κB axis, which may shed light on a possible therapeutic target for SAH.

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Data Availability

All raw data used in this manuscript are available on reasonable request.

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Authors and Affiliations

  1. Department of Neurosurgery, Li Huili Hospital, Ningbo Medical Center, Xingning Road 57th, Yinzhou District, Ningbo, 315000, China

    Jianxiang Jin, Maosong Chen, Hongcai Wang, Shiwei Li, Lei Ma & Boding Wang

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  1. Jianxiang Jin
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  2. Maosong Chen
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  3. Hongcai Wang
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  4. Shiwei Li
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Contributions

J.J., C.M. and W.H. performed the SAH model, RT-PCR and Western blots. L.S. and M.L. performed cell culture and immunostaining. J.J. and W.B. design the project and wrote the main manuscript. All authors reviewed the manuscript.

Corresponding author

Correspondence to Boding Wang.

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This study was performed in line with the Chinese laboratory animal guideline for ethical and animal welfare. Approval was granted by the Institutional Animal Care and Use Committee of Li Huili Hospital, Ningbo Medical Center. (Date: 09/2021/No: 2021065).

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The authors declare no competing interests.

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Jin, J., Chen, M., Wang, H. et al. Schizandrin A attenuates early brain injury following subarachnoid hemorrhage through suppressing neuroinflammation. Mol Biol Rep 51, 236 (2024). https://doi.org/10.1007/s11033-023-08956-7

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