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Interaction between STK33 and autophagy promoted renal cell carcinoma metastasis by regulating mTOR/ULK1 signaling pathway

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Abstract

Background

The roles of STK33 in renal cell carcinoma (RCC) remain unclear. This study was designed to investigate the interaction between STK33 and the autophagy in the RCC.

Methods and results

STK33 was knocked down in 786-O and CAKI-1 cells. Then CCK8, clony formation assay, wound healing assay and Transwell assay were performed to analyze the proliferation, migration and invasion of the cancer cells. In addition, the activation of autophagy was determined using fluorescence, followed by investigating the potential signaling pathways in this process. After STK33 knockdown, the proliferation and migration of cell lines were inhibited, and the apoptosis of renal cancer cells was promoted. Autophagy fluorescence experiment showed that after STK33 knockdown, green LC3 protein fluorescence particles could be seen in the cells. Western blot analysis showed that after STK33 knockdown, there was significant down-regulation in P62 and p-mTOR, as well as significant up-regulation of Beclin1, LC3 and p-ULK1.

Conclusions

STK33 affected autophagy in RCC cells by activating mTOR/ ULK1pathway.

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Data Availability

All data generated or analysed during this study are included in this published article.

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Acknowledgements

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Funding

This work was supported by the Natural Science Foundation of Shandong Province (No. ZR2021MH232), and Science and Technology Development Plan of Tai’an City (No. 2020NS252).

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Authors and Affiliations

  1. Department of Pathology, The Affiliated Taian City Central Hospital of Qingdao University, No. 29, Longtan Road, Taishan District, Taian, 271000, China

    Xiaomei Li, Min Lin, Min Liu, Hong Ye & Shuming Qin

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  1. Xiaomei Li
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  2. Min Lin
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  3. Min Liu
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  4. Hong Ye
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  5. Shuming Qin
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Contributions

Xiaomei Li contributed to the study conception and design. Data collection and analysis were performed by Min Lin, Min Liu, Ye Hong and Shuming Qin. Xiaomei Li wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Xiaomei Li.

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Li, X., Lin, M., Liu, M. et al. Interaction between STK33 and autophagy promoted renal cell carcinoma metastasis by regulating mTOR/ULK1 signaling pathway. Mol Biol Rep 50, 5059–5067 (2023). https://doi.org/10.1007/s11033-023-08396-3

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  • DOI: https://doi.org/10.1007/s11033-023-08396-3

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