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Long non‑coding RNA LINC00460 contributes as a potential prognostic biomarker through its oncogenic role with ANXA2 in colorectal polyps

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Abstract

Background

Long intergenic non-coding RNA 460 (LINC00460) as a potential oncogene and Annexin A2 (ANXA2) as a promoter in different cancer progression processes was considered. A significant relationship between the LINC00460 and ANXA2 has been recently discovered in colorectal cancer (CRC). Therefore, defining molecular biomarkers accompanied by lesion histopathologic features can be a suggestive prognostic biomarker in precancerous polyps. This study aimed to investigate the elusive expression pattern of ANXA2 and LINC00460 in polyps.

Materials and methods

The construction of the co-expression and correlation network of LINC00460 and ANXA2 was plotted. LINC00460 and ANXA2 expression in 40 colon polyps was quantified by reverse transcription-real-time polymerase chain reaction. The receiver operating characteristic (ROC) curve was designed for distinguishing the high-risk precancerous lesion from the low-risk. Further, bioinformatics analysis was applied to find the shared MicroRNA-Interaction-Targets (MITs) between ANXA2 and LINC00460, and the associated pathways.

Results

ANXA2 has a high co-expression rank with LINC00460 in the lncHUB database. Overexpression of ANXA2 and LINC00460 was distinguished in advanced adenoma polyps compared to the adjacent normal samples. The estimated AUC for ANXA2 and LINC00460 was 0.88 − 0.85 with 93%-90% sensitivity and 81%-70% specificity. In addition, eight MITs were shared between ANXA2 and LINC00460. Enrichment analysis detected several GO terms and pathways, including HIF-1α associated with cancer development.

Conclusion

In conclusion, the expression of the ANXA2 and LINC00460 were significantly elevated in pre-cancerous polyps, especially in high-risk adenomas. Collectively, ANXA2 and LINC00460 may be administered as potential prognostic biomarkers in patients with a precancerous large intestine lesion as an alarming issue.

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Abbreviations

CRC:

colorectal cancer

HP:

hyperplastic polyp

AP:

adenomatous polyp

TP:

tubular adenoma polyp

TVP:

tubulovillous polyp

VP:

villus polyp

HRA:

high-risk adenoma

LRA:

low-risk adenoma

lncRNAs:

long noncoding RNAs

LINC00460:

long intergenic noncoding RNA 460

MIT:

microRNA-interaction-target

PPI:

protein-protein interaction

BP:

biological process

MF:

molecular functions

CC:

cellular components

EMT:

epithelial-mesenchymal transition

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Acknowledgements

The authors would like to thank all the staff of the Cancer Department in the Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Funding

This project was completely supported and funded by the Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences.

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Authors and Affiliations

  1. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Farzaneh Alsadat Hosseini & Mehrdad Hashemi

  2. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Leili Rejali, Elahe Daskar-Abkenar, Tannaz Taraz, Nayeralsadat Fatemi & Hamid Asadzadeh-Aghdaei

  3. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Zabihi

  4. Laboratory of Complex Biological Systems and Bio-informatics (CBB), Department of Bioinformatics, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran

    Zahra Salehi

  5. Farhikhtegan Medical Convergence sciences Research Centre, Farhikhtegan Hospital, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran

    Mehrdad Hashemi

  6. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Yeman St, Chamran Expressway, 19857-17413, Tehran, Iran

    Ehsan Nazemalhosseini-Mojarad

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  1. Farzaneh Alsadat Hosseini
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Contributions

Conceptualization FH, LR, MRZ, ZS, EDA, TT, NF, MH, HAA, ENM; Supervision ENM; Visualization FH, LR; Roles/Writing—original draft FH, LR, MRZ, ZS, EDA, TT; Writing—review & editing NF, MH, HAA, ENM. The work reported in the paper has been performed by the authors, unless clearly specified in the text.

Corresponding author

Correspondence to Ehsan Nazemalhosseini-Mojarad.

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Competing interests

Farzaneh alsadat Hosseini, Leili Rejali, Mohammad Reza Zabihi, Zahra Salehi, Elahe Daskar Abkenar, Tanaz Traz, Nayeralsadat Fatemi, Mehrdad Hashemi, Hamid Asadzadeh Aghdaei, Ehsan Nazemalhosseini-Mojarad declare no relevant conflicts of interest. All authors contributed to the review and are responsible for the article content.

Ethics approval

This study was approved by the ethical committee (Ethics No. No.IR.IAU.PS.REC.1400.423) of the Islamic Azad University, Tehran, Iran.

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Informed consent was obtained from all individual participants included in the study.

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Hosseini, F.A., Rejali, L., Zabihi, M.R. et al. Long non‑coding RNA LINC00460 contributes as a potential prognostic biomarker through its oncogenic role with ANXA2 in colorectal polyps. Mol Biol Rep 50, 4505–4515 (2023). https://doi.org/10.1007/s11033-023-08393-6

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