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Effect and mechanism of Fisetin on myocardial damage induced by Patulin

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Abstract

Objectives of the Study

The aim of this study is to investigate whether fisetin can effectively reduce the myocardial damage induced by patulin. This study also aims to reveal the mechanism and target of fisetin in inhibiting myocardial damage.

Materials and Methods

Network pharmacology was used to screen the targets of fisetin on myocardial damage and the regulatory network of active ingredients-drug targets was constructed. GO and KEGG enrichment analyses were performed to screen out the key pathways and targets of fisetin on myocardial damage. Patulin induced apoptosis in H9c2 cardiomyocytes to verify the key targets. The mechanism of fisetin in inhibiting myocardial damage was determined.

Results

FIS can reduce the apoptosis of cardiomyocytes by protecting cardiomyocytes from PAT injury. According to the results of network pharmacology analysis, combined with enzyme activity detection and WB experiment, it was found that the mechanism of FIS to reduce myocardial damage may be related to the P53 signaling pathway, Caspase3/8/9 and Bax/Bcl-2.

Conclusion

FIS plays a protective role in PAT-induced myocardial damage. On the one hand, FIS inhibits the protein overexpression of P53, Caspase-9 and Bax. On the other hand, FIS enhances the protein expression of Bcl-2.

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Ackonwledgements

This work was supported by the National Natural Science Foundation of China (31,760,495); the Nature Fund of Gansu Province(18JR3RA136); Innovation Star Project of Excellent Graduate Students in Gansu Province(2022CZCZ-494).

Funding

This work was supported by the National Natural Science Foundation of China (31760495); the Nature Fund of Gansu Province(18JR3RA136); Innovation Star Project of Excellent Graduate Students in Gansu Province(2022CZCZ-494).

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    Authors and Affiliations

    1. Life Science and Engineering, Lanzhou university of technology, 730050, Gansu, Lanzhou, China

      Dongmei Xu, Baigang Zhang, Chenghui Huang, Jiao Lu, Yang Li & Binggang Fu

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    1. Dongmei Xu
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    2. Baigang Zhang
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    Contributions

    All authors contributed to the study conception and design. Baigang Zhang: Determine the subject, Formal analysis, Investigation, Methodology, Validation, Visualization, Funding support. Dongmei Xu: Determine the subject, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing-review & editing. Chenghui Huang: Determine the subject, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing-review & editing. Lu Jiao: Formal analysis, Investigation, Methodology, Software, Investigation, Methodology, Validation, Writing-original draft, Writing - review & editing. Yang Li: Methodology, Visualization, Formal analysis, Software, Investigation, Project administration. Binggang Fu: Formal analysis, investigation, visualization, software, verification. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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    Correspondence to Baigang Zhang.

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    Dongmei Xu and Baigang Zhang these authors contributed equally to this work.

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    Xu, D., Zhang, B., Huang, C. et al. Effect and mechanism of Fisetin on myocardial damage induced by Patulin. Mol Biol Rep 50, 6579–6589 (2023). https://doi.org/10.1007/s11033-023-08379-4

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