The type IIS restriction enzyme MmeI can cut across a double-strand break

Background Type-IIS restriction enzymes cut outside their recognition sites, allowing them to remove their binding sites upon digestion. This feature has resulted in their wide application in molecular biology techniques, including seamless cloning methods, enzymatic CRISPR library generation, and others. We studied the ability of the Type-IIS restriction enzyme MmeI, which recognizes an asymmetric sequence TCCRAC and cuts 20 bp downstream, to cut across a double-strand break (DSB). Methods and results We used synthetic double-stranded oligos with MmeI recognition sites close to 5′ end and different overhang lengths to measure digestion after different periods of time and at different temperatures. We found that the MmeI binding and cutting sites can be situated on opposite sides of a DSB if the edges of the DNA molecules are held together by transient base-pairing interactions between compatible overhangs. Conclusion We found that MmeI can cut across a DSB, and the efficiency of the cutting depends on both overhang length and temperature.


Introduction
Prokaryotic type IIS restriction-modification systems have provided essential tools for in vitro DNA manipulation and have enabled several prominent molecular techniques, including golden gate and other seamless cloning methods [1,2], MetClo [3], enzymatic CRISPR library generation [4,5], site-directed mutagenesis [6,7], and others. These enzymes are notable because they recognize asymmetric sequences and cut outside their binding site, allowing them to remove their own binding site upon digestion [8]. In addition, many of these enzymes modify their binding site to prevent further recognition at the same location. For example, the type IIS enzyme MmeI recognizes the non-palindromic sequence TCCRAC and cuts 20 bp downstream while subsequently methylating the A in its recognition sequence [9][10][11][12][13]. It is also a type IIL enzyme as it only modifies a

Digestion
MmeI (SAM included), rCutSmart™ buffer, and DNA ladders were ordered from New England Biolabs. Singlestranded (SS) synthetic oligos were acquired from Integrated DNA Technologies (Table 1). Double-stranded oligos with overhangs of different sizes were synthesized by annealing the SS oligos. Each DNA molecule type was synthesized at distinct lengths to allow them to be clearly identified on a gel. The shorter DNA molecule (118 bps) contained a MmeI site with varying lengths of overhang (G, GG, CGG), whereas the longer one (164 bps) did not have any MmeI site and had compatible overhangs (C, CC, CCG). To analyze the effect of overhangs, digestion was done by incubating 130 ng of the shorter DNA substrate (1.7 pmol) and 181 ng of the longer DNA molecule (1.7 pmol) with 4 units (1.28 pmol) of MmeI in rCutsmart buffer (20mM Tris-Albumin, pH 7.9) in a 20 ul reaction. For the DNA (283 bps) containing 1 MmeI site without any DSB, 312 ng of the substrate was used in a 20 ul reaction to ensure an identical molar ratio. To stop each reaction at designated time points, 15 ul of stop solution (50 mM EDTA, pH 8.0,

Gel Electrophoresis
Each in vitro digestion was analyzed by agarose gel electrophoresis with a 4% MetaPhor™ agarose with 1x TAE running buffer and DNA size standards at 90 V for 1 h and 20 min. Images were visualized using an Alpha Innotec gel viewer, and subsequent densitometry analysis was done with ImageJ.

Statistical analysis
All statistical analyses were conducted in R. ANOVA model was used to compare the degree of digestion among the different temperatures and the different overhang length groups (Fig. 1c). Pairwise comparisons were then made using the Tukey test. P-values less than 0.05 were considered to be significant. TRD of another DNA substrate) causes cleavage, even if a DSB is present adjacent to the MmeI recognition sequence [16].
MmeI digestion appears to be at least as rapid as cutting a single DNA with no DSB when 2-or 3-base overhangs are used under the right conditions. We believe that MmeI binding and dimerization may stabilize the endonuclease domain, which allows it to work quickly when the overhang transiently basepairs with another DNA molecule. The effects of temperature on this cutting are somewhat complicated. Surprisingly, cutting at 20 °C was more efficient than at 4 or 37 °C for both 2-and 3-base overhangs, with almost all cutting lost at 37 °C for the 2-base overhangs. Thus, there seems to be an interaction between overhang length and enzymatic activity. While we cannot decisively conclude why this is, it is possible that it is related to two competing factors: enzymatic activity and stability of the transient base-pairing in the overhang. While MmeI is active at 4 °C, its activity increases with temperature. In contrast, transient overhang interactions are more stable at lower temperatures. It is possible that 20 °C represents a "Goldilocks" temperature where MmeI is highly active, but base interactions last long enough for cutting. Further studies are necessary to determine the mechanisms explaining the temperature dependence observed here.
Our findings serve as both an opportunity and a warning for future researchers working with MmeI. On the one hand, unexpected products might be produced by MmeI digestion. Thus, experiments using MmeI binding to temporary linkers must take care to avoid having compatible ends, or unwanted off-products may be produced. On the other hand, this property may have legitimate uses. For example, some protocols call for the ligation of an adapter containing an MmeI site followed by digestion and subsequent removal of the site [4,15]. Our findings indicate that this step can be avoided as MmeI can directly cut the DNA substrate without ligation. formed material preparation, data collection, and analysis. Maliha Tasnim wrote the first draft of the manuscript, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
Funding NIH Grant 1R15HD098969 was awarded to J.T.H. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Results
While developing a protocol using MmeI to generate Cas9 sgRNA libraries, we noticed an unexpected product approximately 15 bp shorter than any expected products or inputs [15]. Further analysis of the digested fragment led to the hypothesis that MmeI can cut across a double-strand break (DSB) mediated by a brief interaction between complementary 2-base overhangs on each DNA molecule. To test this hypothesis, we compared in vitro digestion on synthetic oligos with one containing an MmeI recognition site at the 5′ end and 1-, 2-, or 3-base 5′ overhangs (C/G, CC/GG, and CCG /GGC, respectively) to a DNA molecule of a similar sequence without the DSB. We ran 5-minute incubations at 20 °C (Fig. 1a). The result showed 78.5% digestion with a 2-base overhang, 96.6% with a 3-base overhang, and 90.6% with two sites on the same DNA molecule. A DNA molecule containing a 1-base overhang showed minimal digestion, and blunt-ended oligos with no complementary overhang produced no digestion at all (data not shown). This result demonstrated that cutting across a DSB with overhangs containing at least 2 complementary bases is as efficient or even more efficient than cutting within the same oligo.
To characterize the nature of this hybridization and compare the effect of different temperatures on cleavage efficiency, we ran digestions at 4 °C, 20 °C, and 37 °C for all three different lengths of overhangs (Fig. 1b). Except for the 1 base overhang oligos, all digests had some degree of digestion, although the total amount digested depended on the conditions used. In the case of 2-base and 3-base overhangs, increasing the incubation temperature from 4 to 20 °C increased the amount of digestion. At 37 °C, that amount decreased for both overhang lengths. There was no significant difference between 2-and 3-base overhangs at 4 and 20 °C. However, at 37°, there was a significant increase in digestion for 3-base overhangs compared to 2-base overhangs (Fig. 1c).

Discussion
Our results demonstrate a novel activity for the Type-IIS enzyme MmeI: the ability to cut when a DSB is present between the MmeI recognition site and its cut site 20 bp away (Fig. 1d). Each MmeI has five domains (4 shown). The recognition sequence is embedded between the Methyl transferase (MTase) domain and a target recognition domain (TRD). A helical spacer separates the endonuclease domain from MTase [13]. The disorganized endonuclease domain only becomes stable and cleaves 18-20 bps away when MmeI dimerizes. Once the MmeI trimerizes/tetramerizes, the endonuclease domain of the other MmeI (attached to Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Competing Interests All authors declare they have no financial interests.
Ethical approval This article does not contain any studies with human participants or animals performed by any of the authors.
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