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Morphological and microsatellite marker-based characterization and diversity analysis of novel vegetable soybean [Glycine max (L.) Merrill]

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Abstract

Background

Vegetable soybean seeds are among the most popular and nutrient-dense beans in the world due to their delicious flavor, high yield, superior nutritional value, and low trypsin content. There is significant potential for this crop that Indian farmers do not fully appreciate because of the limited germplasm range. Therefore, the current study aims to identify the diverse lines of vegetable soybean and explore the diversity produced by hybridizing grain and vegetable-type soybean varieties. Indian researchers have not yet published work describing and analysing novel vegetable soybean for microsatellite markers and morphological traits.

Methods and results

Sixty polymorphic SSR markers and 19 morphological traits were used to evaluate the genetic diversity of 21 newly developed vegetable soybean genotypes. A total of 238 alleles, ranging from 2 to 8, were found, with a mean of 3.97 alleles per locus. The polymorphism information content varied from 0.05 to 0.85, with an average of 0.60. A variation of 0.25–0.58 with a mean of 0.43 was observed for Jaccard’s dissimilarity coefficient.

Conclusion

The diverse genotypes identified can be helpful to understand the genetics of vegetable soybean traits and can be used in improvement programs; study also explains the utility of SSR markers for diversity analysis of vegetable soybean. Here, we identified the highly informative SSRs with PIC > 0.80 (satt199, satt165, satt167, satt191, satt183, satt202, and satt126), which apply to genetic structure analysis, mapping strategies, polymorphic marker surveys, and background selection in genomics-assisted breeding.

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Data availability

The data generated or analysed during this study are included in this published article [and its supplementary information files].

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Acknowledgements

Authors sincerely acknowledge the support received from the Head of the Centre of Excellence in Plant Biotechnology and Oilseeds Research Unit, Dr. PDKV, Akola for conduct of the experiments.

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The authors declare that no funds, grants, or other support were received during the research and the preparation of this manuscript.

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Authors and Affiliations

  1. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India

    Priya Pardeshi, Pravin Jadhav, Sanjay Sakhare, Darasing Rathod & Priti Sonkamble

  2. ICAR-Indian Agricultural Research Institute, New Delhi, India

    Rajkumar Zunjare & Ranjit Saroj

  3. Agharkar Research Institute, Pune, India

    Philips Varghese

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Contributions

Conceptualization of research (PJ & SS); Designing of the experiments (PP, PJ & SS); Contribution of experimental materials (PV); Execution of field/lab experiments and data collection (PP, DR & PS); Analysis of data and interpretation (PP, RS & DR); Analysis of SSR data and interpretation (RZ, PP); Preparation of the manuscript (PP, PJ & SS).

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Correspondence to Priya Pardeshi.

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Pardeshi, P., Jadhav, P., Sakhare, S. et al. Morphological and microsatellite marker-based characterization and diversity analysis of novel vegetable soybean [Glycine max (L.) Merrill]. Mol Biol Rep 50, 4049–4060 (2023). https://doi.org/10.1007/s11033-023-08328-1

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