Abstract
Background
Antiphospholipid syndrome (APS) is the main cause of acquired thrombophilia where peripheral circulating cells such as monocytes have a key role. Currently, several studies have linked long non-coding RNAs (lncRNAs) in different inflammatory and autoimmune processes, including lupus. However, the role of lncRNAs in antiphospholipid syndrome is unknown, therefore, we aimed to select and measure expression levels of three lncRNAs based on its abundance in monocytes from APS patients.
Methods
Selection of lncRNAs candidates were carried out based on its abundance in monocytes and their relationship with Perez-Sanchez miRNA signature by using miRNet 2.0 bioinformatic tool, then lncRNAs expression levels was measured in monocytes by RT-qPCR.
Results
This is the first study to report that lncRNAs: FGD5-AS1, OIP5-AS1 and GAS5 are promising candidates for play a role on APS monocytes and they are expressed differently between patients and controls.
Conclusions
OIP5-AS1, FGD5-AS1 and GAS5 are downregulated on monocytes from APS patients.
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References
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Acknowledgements
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Funding
This work was supported with a master fellowship awarded to Carlos A Guzmán-Martín, supported by National Council of Science and Technology (CONACYT 1020089). Instituto Nacional de Cardiología Ignacio Chávez supported publication costs.
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Conceptualization: G-MCA, S-MF, A-GLM, M-MLA, D-LA, G-RJ. Methodology, formal analysis, and investigation: G-MCA, J-VY; Writing -original draft preparation: G-MCA, J-VY, S-MF; funding acquisition: S-MF, and A-GLM; Supervision D-LA, G-RJ, M-MLA. The manuscript has been read and approved for submission by all the named authors.
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All participants signed an informed consent and the protocol was carried out in accordance with institutional guidelines and the principles of the Declaration of Helsinki. This protocol was approved by the local ethics committee (PT-16-039).
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Guzmán-Martín, C.A., Juárez-Vicuña, Y., Domínguez-López, A. et al. lncRNAs dysregulation in monocytes from primary antiphospholipid syndrome patients: a bioinformatic and an experimental proof-of-concept approach. Mol Biol Rep 50, 937–941 (2023). https://doi.org/10.1007/s11033-022-08080-y
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DOI: https://doi.org/10.1007/s11033-022-08080-y