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Lower expression of the equine maternally imprinted gene IGF2R is related to the slow proliferation of hinny embryonic fibroblast in vitro

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Abstract

Background

Proliferation of embryonic fibroblasts under the same cell culture conditions, hinny embryonic fibroblasts (HiEFs) was slower than horse embryonic fibroblast (HEFs), donkey embryonic fibroblasts (DEFs) and mule embryonic fibroblasts (MuEFs). The imprinted genes IGF2 and IGF2R are important for cell proliferation. Therefore, we investigated whether the slower proliferation of HiEFs is related to an aberrant gene expression of IGF2 or its receptors or genes influencing the expression of the IGF2 system.

Methods and Results

Real-time polymerase chain reaction, immunofluorescence and cell starving experiment in HEFs, DEFs, MuEFs and HiEFs revealed that the slower proliferation of HiEF in vitro was related to its lower expression of IGF2R (P < 0.001). Moreover, quantification of allele-specific expression and bisulfate assay confirmed that in both MuEFs and HiEFs, IGF2R had normal maternal imprinting, implying that the imprint aberrant was not involved in the lower IGF2R expression in HiEFs.

Conclusions

The reduction of IGF2R expression in HiEFs is associated with its slower proliferation in vitro.

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Data availability

All data included in this study are available upon request by contact with the corresponding author.

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Funding

This work is supported by Natural Science Foundation of Inner Mongolia Autonomous Region (2019ZD03), the Research project on Applied Technology in Inner Mongolia Autonomous Region (2019GG242), and Youth Fund Project of College of Animal Science, Inner Mongolia Agricultural University (BZCG202112).

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    Authors and Affiliations

    1. College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China

      Xisheng Wang, Nairag Asgenbaatar, Yingchao Shen, Minna Yi, Bilig Zhao, Hong Ren, Toli Davshilt, Tseweendolmaa Ulaangerel, Min Wang, Als Burenbaatar, Shuyue Tian, Bei Li, Manglai Dugarjav & Gerelchimeg Bou

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    1. Xisheng Wang
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    Contributions

    Conceptualization, X.W., G.B. and M.D.; sampling, X.W., T.D., Y.S., N.A., H.R., M.W., A.B., T.U.; methodology, X.W., G.B., M.Y., B.Z.; software, X.W., G.B.; formal analysis, X.W.; writing— original draft preparation, X.W.; writing—review and editing, X.W., G.B.; supervision, G.B., B.L.; project administration, G.B., M.D.; funding acquisition, M.D., G.B. All authors have read and agreed to the published version of the manuscript.

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    Correspondence to Manglai Dugarjav or Gerelchimeg Bou.

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    Inner Mongolia Agricultural University Ethics Committee approved the study.

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    Xisheng Wang and Nairag Asgenbaatar authors contributed equally.

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    Wang, X., Asgenbaatar, N., Shen, Y. et al. Lower expression of the equine maternally imprinted gene IGF2R is related to the slow proliferation of hinny embryonic fibroblast in vitro. Mol Biol Rep 50, 185–192 (2023). https://doi.org/10.1007/s11033-022-07937-6

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