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Cathepsin-B and caveolin-1 gene expressions in oral lichen planus and oral squamous cell carcinoma

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Abstract

Background

Given the importance of the role of cellular changes in the prognosis and diagnosis of malignancies of the head and neck, we examined in this study the gene expressions of cathepsin-B (Cat-B) and caveolin-1 (Cav-1) which are in cell membranes structures involved in carcinogenesis, in oral squamous cell carcinoma (OSCC) and oral lichen planus (OLP) and compared them to controls. We also investigated their relationship to clinicopathological indices.

Methods and Results

In this case–control study, 29 paraffin blocks of OLP patients were compared to 29 paraffin blocks of OSCC samples as well as 28 paraffin blocks of normal oral tissue. Real-time quantitative PCR was performed to determine gene expressions and results were analyzed for their relationship to clinical data using chi-square, Kruskal–Wallis and Mann–Whitney tests.

Results

The mean age of OSCC and OLP patients were 59.24 ± 15.04 and 48.79 ± 14.17 years, respectively. The Cat-B and Cav-1 expressions were significantly higher in OSCC and OLP samples compared to control (p < 0.001). The highest expression was found in OSCC samples. The difference between OLP and control samples for Cat-B and Cav-1 expression was significant. There was no association between the gene expression and age, gender, duration of disease, Thongprasom score, smoking and cutaneous lichen planus. However, the expressions were related to the grade and stage of OSCC samples (P = 0.01, P = 0.02).

Conclusion

The gene expressions of Cat-B and Cav-1 in OSCC were associated with the stage and grade of lesions. Therefore, they appear to be useful in predicting the biological behavior of OSCC and malignant transformation of OLP, although this process is multi factorial and more investigations are needed.

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Data availability

All research data are available upon request.

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Acknowledgements

None.

Funding

This study was carried out in the Department of Dental and Maxillofacial Diseases as well as in the Pathobiology and Immunology Laboratories of Mashhad dentistry faculty in Iran and was supported by Grants from Mashhad university of medical sciences and approved by ethical committee of Mashhad university of medical sciences.

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Authors and Affiliations

  1. Oral and Maxillofacial Disease Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Atessa Pakfetrat & Zahra Delavarian

  2. Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Nooshin Mohtasham & Farnaz Mohajer Tehran

  3. Oral and Maxillofacial Medicine Department, Dental Faculty, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Negin Samiee

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  1. Atessa Pakfetrat
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  2. Zahra Delavarian
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  3. Nooshin Mohtasham
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  4. Farnaz Mohajer Tehran
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  5. Negin Samiee
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Contributions

All listed authors have made enough contributions to this study. NS did the incisional biopsy of the patients, drafted the manuscript and extracted the data. AP and ZD proposed the initial idea and supervised the project, reviewed and authorized the manuscript. NM and FM took part in the pathologic evaluation of the samples.

Corresponding author

Correspondence to Negin Samiee.

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The authors declare that they have no competing interest.

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IR.MUMS.DENTISTRY.REC.1397.039.

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Pakfetrat, A., Delavarian, Z., Mohtasham, N. et al. Cathepsin-B and caveolin-1 gene expressions in oral lichen planus and oral squamous cell carcinoma. Mol Biol Rep 49, 2945–2951 (2022). https://doi.org/10.1007/s11033-022-07115-8

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  • DOI: https://doi.org/10.1007/s11033-022-07115-8

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