Abstract
In order to discover the mechanism of cold stress and identify differentially expressed genes in hypothalamus during cold stress, 4 weeks of age Huainan partridge chickens, Chinese indigenous breed, were chosen for 24 h cold stress and then hypothalamus were isolated and labeled by reverse transcription reaction for cDNA. Labeled cDNA were hybridized with cDNA microarray. After scanning and image processing, the different gene expression profiling of hypothalamus and normal control was investigated. The differentially expressed genes included 334 down-regulated genes and 543 up-regulated genes. In these differentially regulated genes, myosin heavy chain polypeptide 11 (MYH11), light chain polypeptide 9 (MYL9) and tenascin-Y (TNXB), etc., which involved in muscle activity were significantly down-regulated. Genes like cholecystokinin (CCK), neuropeptide Y (NPY), neuropeptide Y receptor 5 (NPY5R), hypocretin receptor 2 (HCRTR2) and hypocretin neuropeptide precursor (HCRT) which responsible for regulation of feeding behavior were significantly up-regulated. In addition, genes responsible for lipid synthesis, like apolipoprotein (APOB) and agouti related protein homolog (AGRP), were also up-regulated. Through pathway analysis using the Kyoto Encyclopedia of Gene and Genomics, during 24 h cold stress, the neuroactive ligand-receptor interaction was firstly initiated in chickens for stimulation of central nervus for feed intake. Adipocytokine signaling pathway was in high activation for supplementation of body energy. Jak-STAT, Ca2+ signaling pathway and other biological reactions were also initiated in response to cold stress. The biological pathways participated in cold stress would provide important information for clarify the mechanism of cold stress and the differentially expressed genes would give much help for screening of candidate genes in breeding of cold stress resistant lines.
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References
Shinder D, Rusal M, Tanny J, Druyan S, Yahav S (2007) Thermoregulatory responses of chicks (Gallus domesticus) to low ambient temperatures at an early age. Poult Sci 86:2200–2209
Yahav S (2000) Domestic fowl—strategies to confront environmental conditions. Avian Poult Biol Rev 11:81–95
Noh SJ, Kang DW, Yoo SB, Lee JY, Kim JY, Kim BT, Lee JH, Jahng JW (2012) Stress-responsive hypothalamic-nucleus accumbens regulation may vary depending on stressors. Indian J Exp Biol 50:447–454
Chen XY, Jiang RS, Geng ZY (2011) Neuroendocrine responses to cold stress in Chinese indigenous breeds from different latitude. J Anim Vet Adv 10:3043–3047
Wang JT, Xu SW (2007) Effects of cold stress on hypothalamic corticotrophin-releasing and thyrotropin-releasing hormone messenger RNA levels in chickens. J Northeast Agric Univ 14:327–332
Selman C, Mclaren JS, Himanka MJ, Speakman JR (2000) Effect of long-term cold exposure on antioxidant enzyme activities in a small mammal. Free Rad Biol Med 28:1279–1285
Hangalapura BN (2006) Cold stress and immunity: do chickens adapt to cold by trading-off immunity for thermoregulation?. Wageningen University, Wageningen
Hangalapura BN, Nieuwland MG, de Vries Reilingh G, Heetkamp MJ, van den Brand H, Kemp B, Parmentier HK (2003) Effects of cold stress on immune responses and body weight of chicken lines divergently selected for antibody responses to sheep red blood cells. Poult Sci 82:1692–1700
Hangalapura BN, Nieuwland MG, de Vries Reilingh G, van den Brand H, Kemp B, Parmentier HK (2004) Durations of cold stress modulates overall immunity of chicken lines divergently selected for antibody responses. Poult Sci 83:765–775
Blahová J, Dobšíková R, Straková E, Suchý P (2007) Effect of low environmental temperature on performance and blood system in broiler chickens (Gallus domesticus). Acta Vet Brno 76:S17–S23
Sonna LA, Fujita J, Gaffin SL, Lilly CM (2002) Effects of heat and cold stress on mammalian gene expression. J Appl Physiol 92:1725–1742
National Research Council (1994) Nutrient requirements of poultry, 9th edn. The National Academies Press, Washington DC
Department of Agriculture, Plant and Animal Health Inspection Service (2004) Blood and tissue collection at slaughtering and rendering establishments, final rule. 9CFR part 71, Federal Register 69, 10137-10151
Tusher V, Tibshirani R, Chu G (2001) Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA 98:5116–5121
Ahmed WA, Tsutsumi M, Nakata S, Mori T, Nishimura Y, Fjisawa T, Kato I, Nakashima M, Kurahashi H, Suzuki K (2012) A functional variation in the hypocretin neuropeptide precursor gene may be associated with obstructive sleep apnea syndrome in Japan. Laryngoscope 122:925–929
Valassi E, Scacchi M, Cavagnini F (2008) Neuroendocrine control of food intake. Nutr Metab Cardiovasc Dis 18:158–168
Iakovleva TV, Makarova EN, Kazantseva A, Bazhan NM (2012) Effect of estradiol on food intake, glucose and fat metabolism in mice C57BL/6 J with mutation yellow at the agouti locus. Ross Fiziol Zh Im I M Sechenova 98:636–645
Hangalapura BN, Nieuwland MG, Buyse J, Kemp B, Parmentier HK (2004) Effect of duration of cold stress on plasma adrenal and thyroid hormone levels and immune responses in chicken lines divergently selected for antibody responses. Poult Sci 83:1644–1649
Hirabayashi M, Ijiri D, Kamei Y, Tajima A, Kanai Y (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146:399–405
Chen XY, Jiang RS, Geng ZY (2011) Differential effects of two indigenous broilers exposed to cold stress and characters of follicle density and diameter. Ital J Anim Sci 10:38–41
Chen XY, Jiang RS, Geng ZY (2012) Cold stress in broiler: global gene expression analysis suggest a major role of CYP genes in cold responses. Mol Biol Rep 39:425–429
Leandro NS, Gonzales E, Ferro JA, Ferro MI, Givisiez PE, Macari M (2004) Expression of heat shock protein in broiler embryo tissues after acute cold or heat stress. Mol Reprod Dev 67:172–177
Shinder D, Luger D, Rusal M, Rzepakovsky V, Bresler V, Yahav S (2002) Early age cold conditioning in broiler chickens (Gallus domesticus): thermotolerance and growth responses. J Therm Biol 27:517–523
Ipek A, Sahan U (2006) Effects of cold stress on broiler performance and ascites susceptibility. Asian Aust J Anim Sci 19:734–738
Ma S, Mifflin SW, Cunningham JT, Morilak DA (2008) Chronic intermittent hypoxia sensitizes acute hypothalamic-pituitary-adrenal stress reactivity and Fos induction in the rat locus coeruleus in response to subsequent immobilization stress. Neuroscience 154:1639–1647
Matteri RL, Carroll JA, Dyer CJ (2000) Neuroendocrine responses to stress. Biol Anim Stress 3:43–74
Pacák K, Palkovits M (2001) Stressor specificity of central neuroendocrine responses: implications for stress-related disorders. Endocr Rev 22:502–548
Roses AD (2009) Stimulation of cholecystokinin-A receptors with Gl181771X: a failed clinical trial that did not test the pharmacogenetic hypothesis for reduction of food intake. Clin Pharmacol Ther 85:362–365
Chandra R, Liddle RA (2007) Cholecystokinin. Curr Opin Endocrinol Diabetes Obes Metab 14:63–67
Kuo LE, Kitlinska JB, Tilan JU, Li L, Baker SB, Johnson MD, Lee EW, Burnett MS, Fricke ST, Kvetnansky R, Herzog H, Zukowska Z (2007) Neuropeptide Y acts directly in the periphery on fat tissue and mediates stress-induced obesity and metabolic syndrome. Nat Med 13:803–811
Palou M, Sánchez J, Rodríguez AM, Priego T, Picó C, Palou A (2009) Induction of NPY/AgRP orexigenic peptide expression in rat hypothalamus is an early event in fasting: relationship with circulating leptin, insulin and glucose. Cell Physiol Biochem 23:115–124
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This work was financially supported by grant from The National Natural Science Foundation for Young Scholars of China (Grant No. 31101710).
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Chen, X.Y., Li, R., Wang, M. et al. Identification of differentially expressed genes in hypothalamus of chicken during cold stress. Mol Biol Rep 41, 2243–2248 (2014). https://doi.org/10.1007/s11033-014-3075-z
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DOI: https://doi.org/10.1007/s11033-014-3075-z