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Inheritance of seed iron and zinc concentrations in common bean (Phaseolus vulgaris L.)

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Abstract

Micronutrients are essential elements needed in small amounts for adequate human nutrition and include the elements iron and zinc. Both of these minerals are essential to human well-being and an adequate supply of iron and zinc help to prevent iron deficiency anemia and zinc deficiency, two prevalent health concerns of the developing world. The objective of this study was to determine the inheritance of seed iron and zinc accumulation in a recombinant inbred line (RIL) population of common beans from a cross of low × high mineral genotypes (DOR364 × G19833) using a quantitative trait locus (QTL) mapping approach. The population was grown over two trial sites and two analytical methods (Inductively Coupled Plasma Spectrometry and Atomic Absorption Spectroscopy) were used to determine iron and zinc concentration in the seed harvested from these trials. The variability in seed mineral concentration among the lines was larger for iron (40.0–84.6 ppm) than for zinc (17.7–42.4 ppm) with significant correlations between trials, between methods and between minerals (up to r = 0.715). A total of 26 QTL were identified for the mineral × trial × method combinations of which half were for iron concentration and half for zinc concentration. Many of the QTL (11) for both iron (5) and zinc (6) clustered on the upper half of linkage group B11, explaining up to 47.9% of phenotypic variance, suggesting an important locus useful for marker assisted selection. Other QTL were identified on linkage groups B3, B6, B7, and B9 for zinc and B4, B6, B7, and B8 for iron. The relevance of these results for breeding common beans is discussed especially in light of crop improvement for micronutrient concentration as part of a biofortification program.

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References

  • Basten CJ, Weir BS, Zeng ZB (2001) QTL cartographer: a reference manual and tutorial for QTL mapping. Department of Statistics, North Carolina State University, Raleigh

    Google Scholar 

  • Beebe S, Gonzalez AV, Rengifo J (2000) Research on trace minerals in the common bean. Food Nutr Bull 21:387–391

    Google Scholar 

  • Beebe SE, Rojas M, Yan X, Blair MW, Pedraza F, Muñoz F et al (2006) Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean. Crop Sci 46:413–423. doi:10.2135/cropsci2005.0226

    Article  CAS  Google Scholar 

  • Benton-Jones J (1989) Plant analysis techniques. Benton-Jones Laboratories, Georgia

    Google Scholar 

  • Blair MW, Pedraza F, Buendia HF, Gaitán-Solís E, Beebe SE, Gepts P et al (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor Appl Genet 107:1362–1374. doi:10.1007/s00122-003-1398-6

    Article  PubMed  CAS  Google Scholar 

  • Bouis HE (2003) Micronutrient fortification of plants through plant breeding: can it improve nutrition in man at low cost? Proc Nutr Soc 62:403–411. doi:10.1079/PNS2003262

    Article  PubMed  Google Scholar 

  • Broughton WJ, Hernandez G, Blair M, Beebe S, Gepts P, Vanderleyden J (2003) Beans (Phaseolus spp.)—model food legumes. Plant Soil 252:55–128. doi:10.1023/A:1024146710611

    Article  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Cichy KA, Forster S, Grafton KF, Hosfield GL (2005) Inheritance of seed zinc accumulation in navy bean. Crop Sci 45:864–870. doi:10.2135/cropsci2004.0104

    Article  CAS  Google Scholar 

  • Forster SM, Moraghan JT, Grafton KF (2002) Inheritance of seed Zn accumulation in navy bean. Annu Rep Bean Improv Coop 45:30–31

    Google Scholar 

  • Frossard E, Bucher M, Machler F, Mozafar A, Hurrell R (2000) Potential for increasing the content and bioavailability of Fe, Zn and Ca in plants for human nutrition. J Sci Food Agric 80:861–879

    Article  CAS  Google Scholar 

  • Gelin JR, Forster S, Grafton KF, McClean P, Rojas-Cifuentes GA (2007) Analysis of seed-zinc and other nutrients in a recombinant inbred population of navy bean (Phaseolus vulgaris L.). Crop Sci 47:1361–1366. doi:10.2135/cropsci2006.08.0510

    Article  CAS  Google Scholar 

  • Graham RD, Welch RM (1999) A new paradigm for world agriculture: productive, sustainable and nutritious food systems to meet human needs. Dev Bull (Canberra) 49:29–32

    Google Scholar 

  • Graham R, Senadhira D, Beebe S, Iglesias C, Monasterio I (1999) Breeding for micronutrient density in edible portions of staple food crops: conventional approaches. Field Crops Res 60:57–80. doi:10.1016/S0378-4290(98)00133-6

    Article  Google Scholar 

  • Graham RD, Welch RM, Bouis HE (2001) Addressing micronutrient malnutrition through enhancing the nutritional quality of staple foods: principals, perspectives and knowledge gaps. Adv Agron 70:77–144. doi:10.1016/S0065-2113(01)70004-1

    Article  Google Scholar 

  • Guzman-Maldonado SH, Martínez O, Acosta-Gallegos J, Guevara-Lara FJ, Paredes-Lopez O (2003) Putative quantitative trait loci for physical and chemical components of common bean. Crop Sci 43:1029–1035

    CAS  Google Scholar 

  • Guzman-Maldonado SH, Acosta-Gallegos J, Paredes-Lopez O (2004) Protein and mineral content of a novel collection of wild and weedy common bean (Phaseolus vulgaris L.). J Sci Food Agric 80:1874–1881. doi:10.1002/1097-0010(200010)80:13<1874::AID-JSFA722>3.0.CO;2-X

    Article  Google Scholar 

  • Hacisalihoglu G, Kochian LV (2003) How do some plants tolerate low levels of soil zinc? Mechansims of zinc efficiency in crop plants. New Phytol 159:341–350. doi:10.1046/j.1469-8137.2003.00826.x

    Article  CAS  Google Scholar 

  • Hacisalihoglu G, Osturk L, Cakmak I, Welch RM, Kochian L (2004) Genotypic variation in common bean in response to zinc deficiency in calcareous soil. Plant Soil 259:71–83. doi:10.1023/B:PLSO.0000020941.90028.2c

    Article  CAS  Google Scholar 

  • House WA, Welch RM, Beebe S, Cheng Z (2002) Potential for increasing the amounts of bioavailable zinc in dry beans through plant breeding. J Sci Food Agric 82:1452–1457. doi:10.1002/jsfa.1146

    Article  CAS  Google Scholar 

  • Islam FMA, Basford KE, Jara C, Redden RJ, Beebe SE (2002) Seed compositional and disease resistance differences among gene pools in cultivated common bean. Genet Resour Crop Evol 49:285–293. doi:10.1023/A:1015510428026

    Article  Google Scholar 

  • Islam FMA, Beebe SE, Muñoz M, Tohme J, Redden RJ, Basford KE (2004) Using molecular markers to assess the effect of introgression on quantitative attributes of common bean in the Andean gene pool. Theor Appl Genet 108:243–252. doi:10.1007/s00122-003-1437-3

    Article  PubMed  Google Scholar 

  • Moraghan JT, Grafton K (1999) Seed zinc concentration and the zinc-efficiency trait in navy bean. Soil Sci Soc Am J 63:918–922

    CAS  Google Scholar 

  • Moraghan JT, Padilla J, Etchevers JD, Grafton K, Acosta-Gallegos JA (2002) Iron accumulation in seed of common bean. Plant Soil 246:175–183. doi:10.1023/A:1020616026728

    Article  CAS  Google Scholar 

  • Polson DE, Adams MW (1970) Differential response of navy beans (Phaseolus vulgaris L.) to zinc. I. Differential growth and elemental composition at excessive Zn levels. Agron J 84:469–474

    Google Scholar 

  • Singh SP, Westermann DT (2002) A single dominant gene controlling resistance to soil zinc deficiency in common bean. Crop Sci 42:1071–1074

    Google Scholar 

  • Vreugdenhil D, Aarts MGM, Koorneef M, Nelissen H, Ernst WHO (2004) Natural variation and QTL analysis for cationic mineral content in seeds of Arabidopsis thaliana. Plant Cell Environ 27:828–839. doi:10.1111/j.1365-3040.2004.01189.x

    Article  CAS  Google Scholar 

  • Wang TL, Domoney C, Hedley CL, Casey R, Grusak MA (2003) Can we improve the nutritional quality of legume seeds? Plant Physiol 131:886–891. doi:10.1104/pp.102.017665

    Article  PubMed  CAS  Google Scholar 

  • Welch RM (2002) Breeding strategies for biofortified staple plant foods to reduce micronutrient malnutrition globally. Symposium: plant breeding: a new tool for fighting micronutrient malnutrition. J Nutr 132:495S–499S (special issue)

    PubMed  Google Scholar 

  • Welch RM, Graham RD (1999) A new paradigm for world agriculture: productive, sustainable and nutritious food systems to meet human needs. Field Crops Res 60:1–10. doi:10.1016/S0378-4290(98)00129-4

    Article  Google Scholar 

  • Welch RM, Graham RD (2004) Breeding for micronutrients in staple food crops from a human nutrition perspective. J Exp Bot 55:353–364. doi:10.1093/jxb/erh064

    Article  PubMed  CAS  Google Scholar 

  • Welch RM, House WA, Beebe S, Cheng Z (2000) Genetic selection for enhanced bioavailable levels of iron in bean (Phaseolus vulgaris L.). J Agric Food Chem 48:3576–3580. doi:10.1021/jf0000981

    Article  PubMed  CAS  Google Scholar 

  • Westermann D, Singh SP (2000) Patterns of response to zinc deficiency in dry bean of different market classes. Annu Rep Bean Improv Coop 43:5–6

    Google Scholar 

  • Wu J, Yuan Y-X, Zhang X-W, Zhao J, Song X, Li Y et al (2008) Mapping QTLs for mineral accumulation and shoot dry biomass under different Zn nutritional conditions in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Plant Soil 310:25–40. doi:10.1007/s11104-008-9625-1

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We are grateful to Octavio Mosquera in the CIAT analytical lab for help with AAS analysis, as well as Teresa Fowles at Waite lab and David Dworak at Baylor College of Medicine for help with ICP analysis. This work was funded in part by CIAT core funds, subprojects of Harvest Plus to MWB, SB and MAG, as well as funds from USDA-ARS under Agreement No. 58-6250-6-003 to MAG. The contents of this publication do not necessarily reflect the views or policies of CIAT or the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Authors and Affiliations

  1. Biotechnology Unit and Bean Project, CIAT – International Center for Tropical Agriculture, 1380 N.W. 78th Ave., Miami, FL, 33126, USA

    M. W. Blair, C. Astudillo & S. E. Beebe

  2. Biotechnology Unit and Bean Project, CIAT – International Center for Tropical Agriculture, A. A. 6713, Cali, Colombia

    M. W. Blair

  3. Department of Pediatrics, Baylor College of Medicine, USDA-ARS Children’s Nutrition Research Center, Houston, TX, USA

    M. A. Grusak

  4. Department of Plant Science, University of Adelaide, Glen Osmond, SA, 5064, Australia

    R. Graham

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  1. M. W. Blair
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  2. C. Astudillo
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  3. M. A. Grusak
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  4. R. Graham
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  5. S. E. Beebe
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Correspondence to M. W. Blair.

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Blair, M.W., Astudillo, C., Grusak, M.A. et al. Inheritance of seed iron and zinc concentrations in common bean (Phaseolus vulgaris L.). Mol Breeding 23, 197–207 (2009). https://doi.org/10.1007/s11032-008-9225-z

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  • DOI: https://doi.org/10.1007/s11032-008-9225-z

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