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Effects of Capparis Spinosa extract on the neuropathic pain induced by chronic constriction injury in rats

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Abstract

Neuropathic pain, a chronic pain condition, puts a considerable burden on its patients. However, different pathophysiological characteristics of neuropathic pain make the current treatment medications insufficient in controlling pain. Identifying treatment effects with Capparis Spinosa hydro-alcoholic extract in an animal model of neuropathic pain. Liquid chromatography-mass spectrometry (LC-MS) was used to identify the components of C. Spinosa hydro-alcoholic extract. To establish a neuropathic pain model, adult male Wistar rats underwent chronic constriction injury (CCI) surgery in their left sciatic nerve. Male wistar rats were divided into four groups: CCI, Sham, CCI with C. Spinosa (100 mg/kg), and CCI with C. Spinosa (200 mg/kg). Rats were treated with a hydro-alcoholic extract from aerial parts of the C. Spinosa (orally, daily) starting from CCI induction until 14 days after. Behavioral tests (mechanical allodynia, cold allodynia, and thermal hyperalgesia) and biochemical tests (IL-1β, TNF-α, MDA, and total thiol) were taken from animals. The LC-MS analysis identified 22 compounds in C. Spinosa extract with the predominance of flavonoids. CCI produced a significant (P < 0.001) increase allodynia (mechanical and cold) and thermal hyperalgesia in comparison with sham group. Oral administration of C. Spinosa significantly (P < 0.05) ameliorated CCI-induced nociceptive pain compared with CCI group. Spinal cord specimens of CCI rats had significant (P < 0.05) elevated inflammation status (↑IL-1β, ↑TNF-α), and significant (P < 0.05) decreased antioxidative status (↑MDA, ↓total thiol) in comparison with the sham group. These changes were reversed following C. Spinosa treatment. C. Spinosa alleviates neuropathic pain by exhibiting antioxidative and anti-inflammatory effects. The responsible components for these effects are possibly the flavonoid compounds in C. Spinosa extract.

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Data availability

The datasets of the current study are available from the corresponding author on request.

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Funding

This research was supported by a grant (961604) from the Vice-Chancellor for Research and Technology, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.

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Authors and Affiliations

  1. Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran

    Hassan Rakhshandeh

  2. Medical Toxicology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Ali Mohammad Pourbagher-Shahri

  3. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Maede Hasanpour & Mehrdad Iranshahi

  4. Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Fatemeh Forouzanfar

  5. Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Fatemeh Forouzanfar

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  1. Hassan Rakhshandeh
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  2. Ali Mohammad Pourbagher-Shahri
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  3. Maede Hasanpour
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Contributions

Hassan Rakhshandeh, and Fatemeh Forouzanfar designed the experiment. Hassan Rakhshandeh, Fatemeh Forouzanfar, and Ali Mohammad Pourbagher-Shahri done the experiments. Maede Hasanpour, and Mehrdad Iranshahi done Liquid chromatography-mass spectrometry apparatus experiment. Fatemeh Forouzanfar, and Ali Mohammad Pourbagher-Shahri wrote the paper. Fatemeh Forouzanfar prepared the final draft of the paper.

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Rakhshandeh, H., Pourbagher-Shahri, A.M., Hasanpour, M. et al. Effects of Capparis Spinosa extract on the neuropathic pain induced by chronic constriction injury in rats. Metab Brain Dis 37, 2839–2852 (2022). https://doi.org/10.1007/s11011-022-01094-2

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