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Role of TRPV4 in matrix stiffness-induced expression of EMT-specific LncRNA

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Abstract

Long non-coding RNAs (LncRNAs) are long (> 200 bases), non-coding, single-stranded RNAs that have emerged as major regulators of gene expression, cell differentiation, development, and oncogenesis. In view of the fact that matrix stiffness plays a role in cellular functions associated with these processes, it is important to ask what role matrix stiffness plays in regulating expression of LncRNAs. In this report, we show that (i) matrix stiffness causes differential expression of epithelial–mesenchymal transition (EMT)-related LncRNAs and mRNAs in primary mouse normal epidermal keratinocytes, (ii) differential expression of EMT-related LncRNAs and mRNAs occurs in response to combined stimulation of transforming growth factor β1 and matrix stiffness, and (iii) transient receptor potential (TRP) channel of the vanilloid subfamily, TRPV4, a matrix stiffness-sensitive ion channel, plays a role in differential expression of EMT-related LncRNAs and mRNAs in response to combined stimulation by TGFβ1 and matrix stiffness. These data identify TRPV4 as a candidate plasma membrane mechanosensor that transmits matrix-sensing signals essential to LncRNA expression. Our results also show that we have established and validated an assay system capable of discovering novel LncRNAs and mRNAs sensitive to matrix stiffening.

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All data are freely available from the corresponding author as per University of Maryland policy of availability of scientific data.

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Funding

This work was supported by National Institute of Health (Grant No 1R01EB024556-01) and National Science Foundation (CMMI-1662776) Grants to Shaik O. Rahaman.

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Authors and Affiliations

  1. Department of Nutrition and Food Science, University of Maryland, College Park, MD, 20742, USA

    Shweta Sharma & Shaik O. Rahaman

  2. Department of Animal and Avian Sciences, University of Maryland, College Park, MD, 20742, USA

    Li Ma

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  1. Shweta Sharma
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by SOR, SS, and LM.

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Correspondence to Shaik O. Rahaman.

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Sharma, S., Ma, L. & Rahaman, S.O. Role of TRPV4 in matrix stiffness-induced expression of EMT-specific LncRNA. Mol Cell Biochem 474, 189–197 (2020). https://doi.org/10.1007/s11010-020-03844-1

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