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Proteomics analysis of plasma membrane from liver sinusoidal endothelial cells after partial hepatectomy by an improved two-dimensional electrophoresis

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Abstract

Liver regeneration is an angiogenesis-associated phenomenon. To identify key plasma membrane (PM) proteins of endothelial cells involved in the initiation of angiogenesis during liver regeneration, the PM of liver sinusoidal endothelial cells (LSEC) at 72 h after partial hepatectomy was enriched by an established in vivo membrane density perturbation method. The differentially expressed membrane proteins compared to those from sham operation were quantified using an improved two-dimensional 16-BAC/SDS-PAGE and identified by LC-MS/MS. Several proteins were further confirmed by cICAT labeling quantitative strategy. A total of 47 proteins were identified including known and novel proteins involved in angiogenesis or liver regeneration, such as inducible nitric oxide synthase, type IV collagen, and integrin beta3. Our results indicated that the combination of the membrane density perturbation strategy and the improved two-dimensional electrophoresis (2-DE) method are useful for investigating the endothelial dysfunctions in vivo.

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Acknowledgments

We thank Dr. Wang Ying for critical comments on the manuscript; we also appreciated Kannan Rangiah and Emer Lucey for their kindly language revision. This work was supported by National Basic Research Program of China (2007CB516809, 2007CB914203) and National Natural Science Foundation of China (30770437).

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Authors and Affiliations

  1. Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, 410081, People’s Republic of China

    Xuanwen Li, Chunliang Xie, Jia Cao, Huobao Deng, Yong Lin, Rui Cao, Jianglin Li, Ping Chen & Songping Liang

  2. Division of Hepatobiliary Disease, Department of General Surgery, the Second Xiangya Hospital of Central South University, Changsha, 410011, People’s Republic of China

    Li Xiong

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  1. Xuanwen Li
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  2. Li Xiong
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  3. Chunliang Xie
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Correspondence to Ping Chen or Songping Liang.

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Xuanwen Li and Li Xiong contributed equally to this work.

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Li, X., Xiong, L., Xie, C. et al. Proteomics analysis of plasma membrane from liver sinusoidal endothelial cells after partial hepatectomy by an improved two-dimensional electrophoresis. Mol Cell Biochem 344, 137–150 (2010). https://doi.org/10.1007/s11010-010-0537-z

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