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Peptide Isolated from Noni Seeds Confers Gastroprotective Effect by Improving Inflammation and Oxidative Stress in Mice

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Abstract

Plant molecules are continuously investigated to prevent and treat inflammatory and ulcerative disorders associated with the gastrointestinal tract, such as gastritis, colitis, mucositis, and ulcers. However, most of the published work is devoted to investigating the therapeutic properties of secondary plant metabolites. This work investigated the gastroprotective activity of a lipid transfer protein isolated from Morinda citrifolia L., named McLTP1, when orally administered to mice from the perspective of its use as a novel peptide-based drug for the prevention and treatment of ulcerative gastric lesions. Pretreatment with McLTP1 at different doses (4, 8, or 16 mg/kg) reduced ethanol-induced gastric lesions (p < 0.05) by 40%, 84%, and 88%, respectively. In ethanol-induced gastric lesions, alterations in the levels of glutathione (GSH) (↑100%; p < 0.05) and a reduction of 45% (p < 0.05) in the levels of malondialdehyde (MDA) were demonstrated after McLTP1 administration (8 mg/kg). McLTP1 showed an anti-inflammatory effect through modulation of the cytokines IL-10 (↑33%) and TNF-α (↓54%) and was able to reduce myeloperoxidase (MPO) levels (↓95%) in gastric tissue. In addition, the gastroprotective effect of McLTP1 also involves the production of nitric oxide. The present findings reveal that McLTP1 has a gastroprotective effect dependent, at least in part, on its anti-inflammatory and antioxidant effects.Please confirm if the author names are presented accurately and in the correct sequence (given name, middle name/initial, family name). Also, kindly confirm the details in the metadata are correct.I confirm that the author names are presented accurately and in the correct sequence.

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Acknowledgements

This work was supported by research grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Award Number: 431827/2016-8) and scholarship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Federal University of Ceará, Campus do Pici Prof. Prisco Bezerra, Mister Hull Avenue n/n, Fortaleza, CE, 60440-900, Brazil

    Francisca Cristiane Nogueira, Andréa Santos Costa, Dyély de Carvalho Oliveira Campos, Rodolpho Glauber Guedes Silva & Hermógenes David de Oliveira

  2. Department of Physiology and Pharmacology, Federal University of Ceará, 1127 Coronel Nunes de Melo Street, Fortaleza, CE, 60430-275, Brazil

    Álvaro Xavier Franco, Nylane Maria Nunes de Alencar & Marcellus Henrique Loiola Ponte de Souza

  3. Department of Morphology, Federal University of Ceará, Delmiro de Farias Street n/n, Fortaleza, CE, 60416-030, Brazil

    Pedro Marcos Gomes Soares

  4. Department of Plant Pathology, Center for Applied Genetic Technologies, University of Georgia, Athens, GA, 30602, USA

    Raquel de Oliveira Rocha

  5. Department of Physiology and Pharmacology, Center of Biosciences, Federal University of Pernambuco, Professor Moraes Rego Avenue, n/n, Recife, PE, 50670-420, Brazil

    Renan Oliveira Silva Damasceno

Authors
  1. Francisca Cristiane Nogueira
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  2. Andréa Santos Costa
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  6. Pedro Marcos Gomes Soares
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  7. Raquel de Oliveira Rocha
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  8. Renan Oliveira Silva Damasceno
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  9. Nylane Maria Nunes de Alencar
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  10. Marcellus Henrique Loiola Ponte de Souza
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  11. Hermógenes David de Oliveira
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Contributions

FCN, HDO, and ROSD conceived the project, designed the experiments, and analyzed the data. FCN, ASC, DCOC, and AXF performed the experiments. FCN, HDO, ROSD, and RSG wrote the manuscript and made critical revisions for intellectual content. PMGS and ROSD analyzed the histopathological slide images. ROR contributed to the English written review. HDO, NMNA, and MHLPS contributed to financial obtaining and support. The authors declare that they have no conflicts of interest.

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Correspondence to Hermógenes David de Oliveira.

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Nogueira, F.C., Costa, A.S., de Carvalho Oliveira Campos, D. et al. Peptide Isolated from Noni Seeds Confers Gastroprotective Effect by Improving Inflammation and Oxidative Stress in Mice. Int J Pept Res Ther 28, 131 (2022). https://doi.org/10.1007/s10989-022-10440-y

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