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Production and Evaluation of a Novel Multi-Epitope Bivalent Vaccine Against Echinococcus multilocaularis Metacestode

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Abstract

Alveolar Echinococcosis is a globally widespread zoonotic disease caused by the larval stage of Echinococcus multilocularis (Em) and is seriously harmful to human health. In our previous studies, we found that Em-EMY162 had good prophylactic and therapeutic effects against Em infection and the dominant epitopes of Em-EMY162 and Em-TSP3 were also identified. In this study, a multi-epitope vaccine LTB-ETBM targeting both Em-EMY162 and Em-TSP3 was designed and constructed, and then the immunogenicity and protective efficacy were evaluated in Em infected mice. The results showed that LTB-ETBM could induce high levels of specific IgG against Em-EMY162 and Em-TSP3 and a mixed Th1/Th2 lymphocyte response to LTB-ETBM. Moreover, the LTB-ETBM significantly inhibited the formation of cysts in mice challenged with 1000 Em protoscoleces. In a therapeutic mouse model injected intraperitoneally with 1000 protoscoleces, vaccination with LTB-ETBM using either Freund's or CpG as an adjuvant significantly decreased the growth of protoscoleces and the formation of cysts. LTB-ETBM may be efficacious for use as a prophylactic or therapeutic agent against Em infection.

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Acknowledgements

The authors gratefully acknowledge the staff at the Altitude Research Center for all the technical support.

Funding

This work was funded by the Province Natural Science Foundation of Qinghai (No. 2017-ZJ-703, Feng Tang), National Natural Science Foundation of China (No.81860299, Tang F), the “Thousand Talents Program” for High-end Innovation of Qinghai Province (Li RL, Tang F), Ningxia Key Research and Development Project (2020BFG02012), and the Qinghai University Undergraduate Innovation and Entrepreneurship Training Project (2019-QH-03).

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Author notes

  1. Runle Li and Mingyuan Xin have contributed equally to this work.

Authors and Affiliations

  1. Research Center for High Altitude Medicine (Qinghai Key Laboratory of Science and Technology; Qinghai-Utah Joint Research Key Lab; Qinghai Key Laboratory for Echinococcosis), Qinghai University, 16# Kunlun Road, Xining, 810001, China

    Runle Li, Mingyuan Xin, Bingwen Hu, Jingwei Ma, Pei Zhou, Lin Feng, Mingquan Pang, Ri-li Ge & Feng Tang

  2. Qinghai University Health Science Center, Qinghai University, 16# Kunlun Road, Xining, 810001, China

    Runle Li, Mingyuan Xin, Bingwen Hu, Jingwei Ma, Pei Zhou, Lin Feng, Mingquan Pang, Ri-li Ge & Feng Tang

  3. Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan, 750004, China

    Kunmei Liu

  4. Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, 750004, China

    Le Guo

  5. Department of Hepatopancreatobiliary Surgery, Affiliated Hospital of Qinghai University, 16# Kunlun Road, Xining, 810001, China

    Haining Fan

Authors
  1. Runle Li
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  2. Mingyuan Xin
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  3. Kunmei Liu
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  4. Bingwen Hu
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  7. Lin Feng
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  10. Haining Fan
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  11. Le Guo
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  12. Feng Tang
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Contributions

Feng Tang, Le Guo, Haining Fan and Ri-li Ge conceived and designed research. Runle Li, Kunmei Liu, Lin Feng and Binwen Hu conducted experiments. Jinwei Ma, Mingyuan Xin, Mingquan Pang, Pei Zhou contributed analytical tools and analyzed data. Runle Li wrote the manuscript. All authors read and approved the manuscript.

Corresponding authors

Correspondence to Le Guo or Feng Tang.

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Conflict of interest

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature that could be construed as influencing the position presented in the manuscript.

Ethical Approval

All animal procedures were approved by were approved by the Animal Ethical and Experimental Committee of Qinghai University (QHDX-2019-09).

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Li, R., Xin, M., Liu, K. et al. Production and Evaluation of a Novel Multi-Epitope Bivalent Vaccine Against Echinococcus multilocaularis Metacestode. Int J Pept Res Ther 28, 114 (2022). https://doi.org/10.1007/s10989-022-10421-1

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