Abstract
Bioactivities including cellular antioxidant activity in HepG2 and dipeptidylpeptidase-IV inhibitory effect of in vitro transepithelial transported peptides with ACE inhibitory activity from cooked chicken breast, namely, APP, KP, KPLL, LL, LVK and LVQ were investigated. HepG2 cells were the most susceptible to KP, however, none of them were toxic up to 100 µM. KP and KPLL showed cytoprotective effect and could reduce intracellular reactive oxygen species (ROS) level in a dose-dependent response (P < 0.05), particularly 100 µM KP showed comparable capacity with 100 µM ascorbic acid as demonstrated by using 2ʹ,7ʹ-dichlorofluorescein-diacetate (DCFH-DA) probe. KP induced expression of SOD1 and GPX1, while CAT appeared to be upregulated by KPLL (P < 0.05). Among the peptides studied, LL with an IC50 of 4.79 mM was the most active peptide against DPP-IV activity through a non-competitive action (Ki = 922.96 µM) as demonstrated by the Lineweaver–Burk plot, suggesting the ability of LL to bind with DPP-IV at various locations other than active site. Molecular docking revealed that LL interacted with the enzyme via van der Waals, salt bridge interactions, and hydrogen bonds.
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Funding
This work was financially supported by the National Research Council of Thailand under the project Food Innovation for Safety and Value Creation of Nakhonchaiburin (SUT3-305-61-12-06). Postdoctoral fellowship of AH was supported by Suranaree University of Technology (SUT), by Thailand Science Research and Innovation (TSRI), and by National Science, Research, and Innovation Fund (NSRF, Project no. 90464).
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JY, the PI, conceived overall experiment and write/correct the manuscript. AH conducted the experiments, data analysis and the manuscript preparation. PS conducted DPP-IV related experiments. PN gave advice on cellular studies. KC performed molecular docking.
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Hamzeh, A., Sangsawad, P., Noisa, P. et al. Bioactivities of In Vitro Transepithelial Transported Peptides from Cooked Chicken Breast. Int J Pept Res Ther 28, 31 (2022). https://doi.org/10.1007/s10989-021-10326-5
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DOI: https://doi.org/10.1007/s10989-021-10326-5