Abstract
Atrial natriuretic peptide (ANP) is a type of peptide hormone secreted by atrial cells, which has many important biological functions and roles. In the current study, we studied the effect of ANP on the activity of epidermal growth factor (EGF). Furthermore, we also explore the cellular properties of EGF in adult retinal pigment epithelial cell line-19 (ARPE-19). For this, we firstly analyzed the cellular properties of EGF on adult retinal pigment epithelial cell line-19 (ARPE-19), the results indicated that EGF/EGFR (epidermal growth factor receptor) internalized into the ARPE-19 cells in a time-dependent manner. In addition, we also found that EGF/EGFR can be transported into the cell nuclei of ARPE-19 in a time-dependent manner in ARPE-19 cells. Furthermore, we analyzed the effect of ANP on EGF’s activity, and found that EGFR-mediated signaling was activated with EGF stimulation in a dose-dependent manner, but the intracellular signaling pathways were inhibited by ANP treatment. Additionally, we further analyzed the potential mechanism by which ANP affects the EGF signaling pathway, and the results showed that ANP alone has no effect on total EGFR expression by Western-blot assay. Next, we further evaluated the EGFR stability on the cell membrane, and the results indicated that membrane-localized EGFR expression was significantly down-regulated under ANP treatment, although the total EGFR expression was not changed. In short, the current research lays the foundation for studying the regulatory relationship between ANP and EGF.
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This work was supported by the Second Affiliated Hospital of Army Medical University.
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Conceived and designed the experiments: JL and ZY performed the experiments: JL, YW, JC and SZ, Analyzed the data: JL and YZ.
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Li, J., Wang, Y., Chen, J. et al. Atrial Natriuretic Peptide (ANP) Regulates the Biological Activity of EGF/EGFR System in Adult Retinal Pigment Epithelial Cell. Int J Pept Res Ther 27, 2559–2570 (2021). https://doi.org/10.1007/s10989-021-10273-1
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DOI: https://doi.org/10.1007/s10989-021-10273-1