Abstract
Pulmonary arterial hypertension (PAH) leads to changes in the pump function of the heart and causes right-sided myocardial hypertrophy and heart failure. This study was the first to compare the contractile characteristics of the multicellular myocardial preparations of the right atrium (RA) and right ventricle (RV) of male rats from the control group (CON) and the group with monocrotaline (MCT)-induced hypertrophy at the molecular and multicellular levels. In both RA and RV in MCT-treated rats, the fraction of motile filaments and the maximum sliding velocity of actin and reconstituted thin filaments over myosin decreased, and the ratio of α-/β-myosin heavy chains (MHC) shifted towards β-MHC. In the RA strips and RV trabeculae, the maximum shortening velocity, the extent of muscle shortening, the amplitude of isometric stress, the amount of work decreased. PAH leads to a greater drop in right atrial contractility than that of the ventricle.
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Acknowledgements
The research was supported by Russian Science Foundation [grant number 21-15-00169]. This work was performed using the equipment of the Shared Research Center of Scientific Equipment SRC IIP UrB RAS.
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Oksana P. Gerzen performed research, analyzed data, wrote the draft paper, and edited final manuscript; Ruslan V. Lisin performed research, analyzed data, and reviewed final manuscript; Alexander A. Balakin performed research, analyzed data, wrote the draft paper, and edited final manuscript; Elena A. Mukhlynina performed research, analyzed data, and reviewed final manuscript; Daniil A. Kuznetsov performed research, analyzed data, and reviewed final manuscript; Larisa V. Nikitina performed research, analyzed data, and reviewed final manuscript; Yuri L. Protsenko designed research, analyzed data, wrote the draft paper, and edited final manuscript. All authors approved the final manuscript.
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Gerzen, O.P., Lisin, R.V., Balakin, A.A. et al. Characteristics of the right atrial and right ventricular contractility in a model of monocrotaline-induced pulmonary arterial hypertension. J Muscle Res Cell Motil 44, 299–309 (2023). https://doi.org/10.1007/s10974-023-09651-7
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DOI: https://doi.org/10.1007/s10974-023-09651-7