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β-cyclodextrin based electrospun nanofibers for arginase immobilization and its application in the production of L-ornithine

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Abstract

Electrospinning is the most effective and efficient method for nanofiber production. In this study, polyvinyl alcohol (PVA)/β-cyclodextrin (β-CD) and PVA/β-CD/Mn2+ electrospun nanofibers were synthesized for use in arginase immobilization. The structural and morphological analysis of the β-cyclodextrin based nanofibers were determined by FTIR, XRD, TGA and SEM. Arginase was immobilized on the β-CD based nanofibers by adsorption and cross-linking methods. After immobilization β-CD based nanofibers (especially PVA/β-CD/Mn2+) demonstrated remarkable improvement in stability properties. When the free arginase lost almost all of its activity after 60 min at 80 °C, both arginase immobilized β-cyclodextrin based nanofibers protected nearly 80–90% activity at the same time. Arginase immobilized β-CD based nanofibers retained 95% of their activity in the acidic region, free arginase maintained only 10–20% of its activity. Arginase immobilized PVA/β-CD and PVA/β-CD/Mn2+ nanofibers protected approximately 50% of their activity after 16 and 20 reuses, respectively. L-ornithine production performance of arginase immobilized PVA/β-CD/Mn2+ nanofiber was found to be 64% even after 5th cycles.

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Acknowledgements

This study is a part of Ceyhun Işık’s PhD thesis and was supported by The Scientific and Technological Research Council of Turkey (Project no: TUBITAK‐218Z092) and Mugla Sitki Kocman University Research Fund (Project no: MUBAP 19/076/04/1).

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The authors would like to thank the The Scientific and Technological Research Council of Turkey (Project no: TUBITAK‐218Z092) and Mugla Sitki Kocman University Research Fund (Project no: MUBAP 19/076/04/1).

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  1. Faculty of Science, Chemistry Department, Muğla Sıtkı Koçman University, Muğla, Turkey

    Ceyhun Işik & Mustafa Teke

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Işik, C., Teke, M. β-cyclodextrin based electrospun nanofibers for arginase immobilization and its application in the production of L-ornithine. J Polym Res 29, 121 (2022). https://doi.org/10.1007/s10965-022-02968-w

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