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Colloidal bioactive nanospheres prepared from natural biomolecules, catechin and L-lysine

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Abstract

Recently, major attention has been devoted to exploring and increase biomedical applications of catechins by directly employing them as constituents of nano-vehicles. Here, (+)-catechin (CAT) was integrated with another benign biomolecule, L-Lysine (LYS) using formaldehyde (FA) via single-step Mannich condensation reaction and self-assembled supramolecular CAT-LYS networks were synthesized. By using various molar feed ratios of CAT, LYS, and FA (CAT:LYS:FA), different formulations of CAT-LYS particles were obtained as CAT-LYS-1(1:1:1), CAT-LYS-2(2:1:1), CAT-LYS-3(1:2:1), and CAT-LYS-4(1:1:2) particles. The CAT-LYS-4 particles with the highest gravimetric yield of 68.9 ± 6.0% and 783.6 ± 56.6 nm hydrodynamic diameter was chosen for bioactivity studies. The CAT-LYS-4 particles exhibited 190.4 ± 1.3 µg/mL CAT-equivalent antioxidant capacity at 1000 µg/mL concentration with TEAC value of 0.24 ± 0.01 µmole Trolox-equivalent/g antioxidant activity. They showed 16.81 ± 3.47% Fe(II) chelation capacity at 350 µg/mL and 185.8 ± 22.8 µmole Fe(III) reducing power at 500 µg/mL concentration. Moreover, the CAT-LYS-4 particles retained more than half of the α-glucosidase inhibition activity of CAT in particulate form. Besides, a 50-fold improvement was achieved on the hemolytic blood compatibility of CAT-LYS-4 particles upon integration of LYS into CAT backbone (4.7 ± 1.2% at 250 µg/mL) compared to hemolysis ratio of native CAT molecules. They did not show coagulation effects up to 500 µg/mL concentration with > 94% clotting indices. Hence, the CAT-LYS particles with enhanced blood compatibilities and well-retained inherent bioactivities of their precursors in 3D colloidal particulate structures can serve as natural biocolloids for drug/active molecule transport applications in biomedicine.

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Acknowledgements

The authors gratefully acknowledge the financial support provided for this study by the Scientific Research Commission of Canakkale Onsekiz Mart University (COMU FHD-2020-3310).

Funding

This study was financially supported by Scientific Research Commission of Canakkale Onsekiz Mart University (COMU FHD-2020-3310).

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Authors and Affiliations

  1. Department of Chemistry & Nanoscience and Technology Research and Application Center, Canakkale Onsekiz Mart University Terzioglu Campus, 17100, Canakkale, Turkey

    Mehmet Can & Nurettin Sahiner

  2. Fashion Design, Canakkale Applied Science Faculty, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100, Canakkale, Turkey

    Mehtap Sahiner

  3. Department of Chemical and Biomolecular Engineering, University of South Florida, Tampa, FL, 33620, USA

    Nurettin Sahiner

  4. Department of Ophthalmology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs B. Downs Blv., MDC 21, Tampa, FL, 33612, USA

    Nurettin Sahiner

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  1. Mehmet Can
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  2. Mehtap Sahiner
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  3. Nurettin Sahiner
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Correspondence to Nurettin Sahiner.

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Can, M., Sahiner, M. & Sahiner, N. Colloidal bioactive nanospheres prepared from natural biomolecules, catechin and L-lysine. J Polym Res 29, 88 (2022). https://doi.org/10.1007/s10965-022-02941-7

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