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Investigating the Protective Role of Biochaga Drug on Structural Changes of Bovine Serum Albumin in the Presence of Methyl tert-butyl Ether

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Abstract

Background: The health benefits of natural products have a long history. Chaga (Inonotus obliques) is used in traditional medicine and is an essential antioxidant for protecting the body from oxidants. Reactive oxygen species (ROS) are produced routinely due to metabolic processes. However, environmental pollution factors such as methyl tert-butyl ether (MTBE) can increase oxidative stress in the human body. MTBE is widely used as a fuel oxygenator that can harm health. The widespread use of MTBE has posed significant threats to the environment by polluting environmental resources, including groundwater. This compound can accumulate in the bloodstream by inhaling polluted air, with a strong affinity for blood proteins. The primary mechanism of MTBE’s harmful effects is ROS production. The use of antioxidants may help reduce MTBE oxidation conditions. The present study proposes that biochaga, as an antioxidant, can reduce MTBE damage in the bovine serum albumin (BSA) structure. Methods and Results: This study investigated the role of different concentrations of biochaga in the structural change of BSA in the presence of MTBE by biophysical methods such as UV-Vis, fluorescence, FTIR spectroscopy, DPPH radical inhibition method, aggregation test, and molecular docking. Research at the molecular level is critical to investigate the structural change of proteins by MTBE and the protective effect of the ideal dose (2.5 µg/ml) of biochaga. Conclusion: the results of spectroscopic examinations showed that the concentration of 2.5 µg/ml of biochaga has the least destructive effect on the structure of BSA in the presence and absence of MTBE, and it can play as an antioxidant.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Niloofar Sepehri, Elmira Parchizadeh & Parvaneh Maghami

  2. Department of Biology, Faculty of Basic Science, Azarbaijan Shahid Madani University, Tabriz, Iran

    Masoumeh Valipour

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All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by NiloofarSepehri, Masoumeh Valipour, Elmira Parchizadeh, and Parvaneh Maghami. The first draft of the manuscript was written by NiloofarSepehri, and all authors commented on previous versions. All authors read and approved the final manuscript.

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Sepehri, N., Valipour, M., Parchizadeh, E. et al. Investigating the Protective Role of Biochaga Drug on Structural Changes of Bovine Serum Albumin in the Presence of Methyl tert-butyl Ether. Protein J 42, 112–124 (2023). https://doi.org/10.1007/s10930-023-10102-y

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