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Structure and Bioactivity of Purified Polysaccharides of Cassia occidentalis Linn. from Different Geographical Origins

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Abstract

As the area of origin may influence the polysaccharide properties of plants, we aimed to comprehensively compare composition of polysaccharides from Cassia occidentalis Linn. (a “famine food”) grown in different regions in China. We collected Cassia occidentalis Linn. specimens form Yunnan and Shandong and investigated previously unreported structural and functional differences among their purified fractions. Crude polysaccharide yields obtained from C. occidentalis from Yunnan (11.07 ± 1.09%) and Shandong (3.15 ± 0.45%) were significantly different (P < 0.05). No compositional differences were observed between the ration of purified fractions obtained via an ion exchange column, indicating that the polysaccharides obtained from both regions were similar in composition. However, Fourier-transform infrared spectroscopy, High-performance liquid chromatography, Nuclear magnetic resonance spectroscopy, and atomic force microscope images revealed several structural differences between the purified polysaccharides obtained from Yunnan and Shandong. Evaluation of antioxidant abilities using the radical scavenging method and the H2O2-induced cell oxidative damage model also revealed structural differences. The study illustrated that the effects exerted by environmental abiotic factors on the composition of these plants.

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Data Availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank shiyanjia lab (www.shiyanjia.com) for the support of AFM analysis.

Funding

The Project is sponsored by "Liaoning BaiQianWan Talents Program (2111520241)".

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China

    Shuoyang Zhang, XinHang Li, Xinlei Lu, Cai Long, Yunli Zhao & Zhiguo Yu

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  1. Shuoyang Zhang
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  2. XinHang Li
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  4. Cai Long
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  5. Yunli Zhao
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  6. Zhiguo Yu
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by SZ, XL and XL. The first draft of the manuscript was written by SZ and revised by ZY, YZ and CL. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Cai Long or Zhiguo Yu.

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Zhang, S., Li, X., Lu, X. et al. Structure and Bioactivity of Purified Polysaccharides of Cassia occidentalis Linn. from Different Geographical Origins. J Polym Environ 31, 5137–5151 (2023). https://doi.org/10.1007/s10924-023-02936-5

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