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Development of Nanocomposite Films Based on Chitosan and Gelatin Loaded with Chitosan-Tripolyphosphate Nanoparticles: Antioxidant Potentials and Applications in Wound Healing

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Abstract

Recently, there is an increasing interest to prepare wound healing agents based on bio-polymers, due to their non-toxicity, biocompatibility and biodegradability. Despite this interest, nanocomposite films were prepared based on chitosan and gelatin reinforced with chitosan-sodium tripolyphosphate nanoparticles (CS-NPs). Nanoparticles were incorporated onto films at different weight ratios (2, 4 and 6 wt%, dry base). Nanocomposite films enriched with nanoparticles were characterized by a higher UV-barrier property, compared to the control film. Films were characterized in terms of physical, mechanical and thermal properties, as well as their bioactivity. An increase in the tensile strength was observed with CS-NPs incorporation, while the elongation at break was adversely affected by nanoparticles amount. Thermal analyses confirmed the compatibility and miscibility between the different film components. Further, CS-NPs addition increased the surface wettability (polar component and surface free energy) of nanocomposite films. The biological properties of films, as well as their wound healing ability were explored. Results showed that nanoparticles incorporation improved significantly the antioxidant and antibacterial efficacies of nanocomposite films. Moreover, in-vivo wound healing test showed that the rate of wound reduction was greatly elevated with a rapid re-epithelialization for treated group with nanocomposite films. Consequently, films were suitable and promising alternatives biomaterials for wound healing and skin regeneration.

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Acknowledgements

The present work is supported by the Utique PHC program (Project SeaCoatPack) N°39290YK of Campus France and No 18G0903 of the CMCU funded by the Ministry of Higher Education and Scientific Research of Tunisia and Ministry of Higher Education, Research and Innovation of France and French Embassy in Tunisia. The authors wish to thank the colleagues from the PAM-PAPC Laboratory for the help and precious collaboration, and to thank Engineering School of Materials (ESIREM) of the University of Bourgogne, DIJON for the facilitated access to the equipment and devices. The equipments involved in this work were also co-funded by the Regional Council of Bourgogne–Franche Comté and the “Fonds Européen de Développement Régional (FEDER)”.

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Authors and Affiliations

  1. Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, PB 1173, 3038, Sfax, Tunisia

    Sawssen Hajji, Naourez Ktari & Moncef Nasri

  2. Department of Life Sciences, Faculty of Science of Gabes, 6029, Gabès, Tunisia

    Naourez Ktari

  3. Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax, 3018, Sfax, Tunisia

    Riadh Ben Salah

  4. Faculty of Science of Sfax - LMSE, University of Sfax, 3018, Sfax, Tunisia

    Sami Boufi

  5. Univ. Bourgogne Franche-Comté - Agrosup Dijon, UMR PAM A02-102, 1 Esplanade Erasme, 21000, Dijon, France

    Frédéric Debeaufort

  6. Dpt of Bioengineering, IUT Dijon-Auxerre, Université de Bourgogne, PB 17867, 21078, Dijon Cedex, France

    Frédéric Debeaufort

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  1. Sawssen Hajji
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Correspondence to Sawssen Hajji.

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Hajji, S., Ktari, N., Ben Salah, R. et al. Development of Nanocomposite Films Based on Chitosan and Gelatin Loaded with Chitosan-Tripolyphosphate Nanoparticles: Antioxidant Potentials and Applications in Wound Healing. J Polym Environ 30, 833–854 (2022). https://doi.org/10.1007/s10924-021-02239-7

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