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Differential Transcriptional Expression of PPARα, PPARγ1, and PPARγ2 in the Peritoneal Macrophages and T-cell Subsets of Non-obese Diabetic Mice

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Abstract

Background

The peroxisome proliferator-activated receptors (PPARs) have been implicated in immune regulation. We determined the transcriptional expression of the three isoforms, PPARα, PPARγ1, and PPARγ2 in the peritoneal macrophages, CD4- and CD8-positive lymphocytes in non-obese diabetic (NOD) mice at 5 and 10 weeks of age as well as at diabetic stage.

Results

Compared to the non-obese diabetic resistant (NOR) mice, the peritoneal macrophages of NOD mice expressed increased levels of PPARα but reduced levels of PPARγ2, while PPARγ1 expression was unchanged in all age groups. CD4-positive lymphocytes expressed low levels of PPARα in diabetic NOD mice and greatly reduced expression of PPARγ2 in all age groups. Unlike peritoneal macrophages and CD4-positive cells, the CD8-positive cells expressed low levels of PPARγ1 in diabetic NOD mice but no difference in PPARα and PPARγ2 expression was observed compared to NOR mice.

Conclusion

The current findings may suggest an important regulatory role of PPARs in the pathogenesis of autoimmune diabetes.

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Acknowledgments

This study was supported by the IRPA Grant (No. 305/PPSP/6110251) provided by the Ministry of Science, Technology and Environment, Malaysia. MAK was supported by the National Science Fellowship under the same ministry. We are grateful to Mr. Jamaruddin Mat Asan for his expert technical assistance in flow cytometry.

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Authors and Affiliations

  1. School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia

    Nik-Soriani Yaacob & Mohd Arifin Kaderi

  2. School of Health Sciences, Universiti Sains Malaysia Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia

    Mohd-Nor Norazmi

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  1. Nik-Soriani Yaacob
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  2. Mohd Arifin Kaderi
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Correspondence to Mohd-Nor Norazmi.

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Yaacob, NS., Kaderi, M.A. & Norazmi, MN. Differential Transcriptional Expression of PPARα, PPARγ1, and PPARγ2 in the Peritoneal Macrophages and T-cell Subsets of Non-obese Diabetic Mice. J Clin Immunol 29, 595–602 (2009). https://doi.org/10.1007/s10875-009-9300-1

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