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Development of a decellularized liver matrix-based nanocarrier for liver regeneration after partial hepatectomy

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Abstract

Integrating carrier drug therapy with recent advances in liver tissue regeneration, this paper proposes a novel nanomedicine-based approach to drug delivery, with the aim of enhancing the regenerative capacity of hepatocytes and improving liver function after partial hepatectomy. The proposed decellularized liver matrix provides excellent biocompatibility and similarity to natural liver components, while the molecules encapsulated in the carrier (tannic acid) promote liver regeneration. In in vitro cultures, the 0.001 mg/mL TA-mPEG-DLM group consistently outperformed the blank group in terms of albumin synthesis: Day 1 (11.1 ± 2.4 vs. 8.2 ± 0.7), Day 3 (5.8 ± 1.3 vs. 4.2 ± 0.4), and Day 5 (1.6 ± 0.3 vs. 1.2 ± 0.2) (μg/100% proliferation rate/well/day). In a liver-injured in vivo model, the 0.5 mg/mL TA-mPEG-DLM group outperformed the blank group in terms of relative liver weight: Day 3 (3.50 ± 0.09% vs. 3.22 ± 0.03%) and Day 7 (4.13 ± 0.21% vs. 3.72 ± 0.06%). Furthermore, the TA-mPEG-DLM group outperformed the blank group in terms of Ki-67 expression in hepatocytes on Day 7 (192.7 ± 34.1% vs. 150.4 ± 11.6%). Taken together, these findings indicate that the proposed drug delivery strategy is a promising approach to liver regeneration following partial hepatectomy with potential for clinical translation.

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Acknowledgements

The authors would like to thank National Taiwan University Hospital and National Taiwan University for their financial support (112-UN0076).

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National Taiwan University Hospital and National Taiwan University (112-UN0076).

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Authors and Affiliations

  1. Department of Biomechatronics Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan

    Yu-Chuan Chiu, Yong-Heng Lin, Wei-Rong Yin & Yung-Te Hou

  2. Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, No. 1, Changde Street, Taipei, 100229, Taiwan

    Kai-Wen Huang

  3. Center of Functional Image and Interventional Therapy, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan

    Kai-Wen Huang

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  1. Yu-Chuan Chiu
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Contributions

Y-CC—Methodology, Investigation, Validation. K-WH—Supervision. Writing—review & editing. Y-HL—Investigation, Validation. W-RY—Investigation, Validation. Y-TH—Supervision, Project administration, Writing—review & editing.

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Correspondence to Yung-Te Hou.

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The experimental protocol was reviewed and approved by the Ethics Committee on Animal Experiments of National Taiwan University (IACUC Approval No.: NTU-110-EL-00055).

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Chiu, YC., Huang, KW., Lin, YH. et al. Development of a decellularized liver matrix-based nanocarrier for liver regeneration after partial hepatectomy. J Mater Sci 58, 15162–15180 (2023). https://doi.org/10.1007/s10853-023-08971-w

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