Skip to main content

Advertisement

SpringerLink
Log in

Preimplantation HLA haplotyping using tri-, tetra-, and pentanucleotide short tandem repeats for HLA matching

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To aid couples wishing to conceive children who are HLA matched to a sibling in need of a hematopoietic progenitor cell transplant, we developed a preimplantation HLA haplotype analysis of embryos that utilizes tri-, tetra-, and pentanucleotide STR markers.

Methods

For preimplantation HLA genotyping, we use polymorphic STR markers located across the HLA and flanking regions, selecting exclusively tri-, tetra-, and pentanucleotide repeats. These markers can be resolved using either capillary electrophoresis (CE) or polyacrylamide gels.

Results

We have developed 43 reliable STR markers for preimplantation HLA matching. Selected STR markers enabled unambiguous identification of embryos whose HLA haplotypes were matched with the affected patient using polyacrylamide gel or capillary electrophoresis.

Conclusions

The use of tri-, tetra-, and pentanucleotide repeat markers and polyacrylamide gels for STR genotyping in HLA matching is a simple and cost effective approach to clinical testing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Bick DP, Lau EC. Preimplantation genetic diagnosis. Pediatr Clin North Am 2006;53(4):559–77.

    Article  PubMed  Google Scholar 

  2. Verlinsky Y, Rechitsky S, Schoolcraft W, Strom C, Kuliev A. Preimplantation diagnosis for Fanconi anemia combined with HLA matching. JAMA 2001;285(24):3130–3.

    Article  PubMed  CAS  Google Scholar 

  3. Grewal SS, Kahn JP, MacMillan ML, Ramsay NK, Wagner JE. Successful hematopoietic stem cell transplantation for Fanconi anemia from an unaffected HLA-genotype-identical sibling selected using preimplantation genetic diagnosis. Blood 2004;103(3):1147–51.

    Article  PubMed  CAS  Google Scholar 

  4. Kuliev A, Rechitsky S, Tur-Kaspa I, Verlinsky Y. Preimplantation genetics: improving access to stem cell therapy. Ann N Y Acad Sci 2005;1054:223–7.

    Article  PubMed  Google Scholar 

  5. Van de Velde H, Georgiou I, De Rycke M, Schots R, Sermon K, Lissens W, et al. Novel universal approach for preimplantation genetic diagnosis of β-thalassaemia in combination with HLA matching of embryos. Hum Reprod 2004;19(3):700–8.

    Article  PubMed  CAS  Google Scholar 

  6. Fiorentino F, Kahraman S, Karadayi H, Biricik A, Sertyel S, Karlikaya G, et al. Short tandem repeats haplotyping of the HLA region in preimplantation HLA matching. Eur J Hum Genet 2005;13(8):953–8.

    Article  PubMed  CAS  Google Scholar 

  7. Verlinsky Y, Rechitsky S, Sharapova T, Laziuk K, Barsky I, Verlinsky O, et al. Preimplantation diagnosis for immunodeficiencies. Reprod Biomed Online 2007;14(2):214–23.

    PubMed  CAS  Google Scholar 

  8. Fiorentino F, Biricik A, Karadayi H, Berkil H, Karlikaya G, Sertyel S, et al. Development and clinical application of a strategy for preimplantation genetic diagnosis of single gene disorders combined with HLA matching. Mol Hum Reprod 2004;10(6):445–60.

    Article  PubMed  CAS  Google Scholar 

  9. Olejniczak M, Krzyzosiak WJ. Genotyping of simple sequence repeats—factors implicated in shadow band generation revisited. Electrophoresis 2006;27(19):3724–34.

    Article  PubMed  CAS  Google Scholar 

  10. Cullen M, Malasky M, Harding A, Carrington M. High-density map of short tandem repeats across the human major histocompatibility complex. Immunogenetics 2003;54(12):900–10.

    PubMed  CAS  Google Scholar 

  11. Benson G. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acid Res 1999;27(2):573–80.

    Article  PubMed  CAS  Google Scholar 

  12. Swanson A, Strawn E, Lau E, Bick D. Preimplantation genetic diagnosis: technology and clinical applications. WMJ 2007;106(3):145–51.

    PubMed  Google Scholar 

  13. Krisher RL, Gibbons JR, Gwazdauskas FC. Nuclear transfer in the bovine using microinjected donor embryos: assessment of development and deoxyribonucleic acid detection frequency. J Dairy Sci 1995;78:1282–88.

    Article  PubMed  CAS  Google Scholar 

  14. Davies JK, Guinan EC. An update on the management of severe idiopathic aplastic anaemia in children. Br J Haematol 2007;136(4):549–64.

    Article  PubMed  CAS  Google Scholar 

  15. Chakraborty R, Kimmel M, Stivers D, Davison L, Deka R. Relative mutation rates at di-, tri-, and tetranucleotide microsatellite loci. Proc Natl Acad Sci USA 1997;94:1041–46.

    Article  PubMed  CAS  Google Scholar 

  16. Korzebor A, Zamani M, Nouri K, Modarressi MH. Statistical analysis of six STR loci located in MHC region in Iranian population for preimplantation genetic diagnosis. Int J Immunogenet 2007;34(6):441–3.

    Article  PubMed  CAS  Google Scholar 

  17. Perz JB, Szydlo R, Sergeant R, Sanz J, O’Shea D, Khan T, et al. Impact of HLA class I and class II DNA high-resolution HLA typing on clinical outcome in adult unrelated stem cell transplantation after in vivo T-cell depletion with Alemtuzumab. Transpl Immunol 2007;18:179–85.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Medical Genetics, Department of Pediatrics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA

    Sarah L. Bick, David P. Bick, Brent E. Wells & Eduardo C. Lau

  2. Individualized Medicine Institute, Children’s Research Institute, Children’s Hospital and Health System of Wisconsin, Milwaukee, WI, 53226, USA

    Sarah L. Bick, David P. Bick, Brent E. Wells & Eduardo C. Lau

  3. Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI, 53226, USA

    Sarah L. Bick, David P. Bick, Brent E. Wells & Eduardo C. Lau

  4. Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA

    David P. Bick & Estil Y. Strawn

  5. Reproductive Medicine Clinic, Froedtert Memorial Lutheran Hospital, 9200 West Wisconsin Avenue, Milwaukee, WI, 53226, USA

    Mark R. Roesler & Estil Y. Strawn

  6. Children’s Research Institute, TBRC Room C2450, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA

    Eduardo C. Lau

Authors
  1. Sarah L. Bick
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David P. Bick
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brent E. Wells
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark R. Roesler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Estil Y. Strawn
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Eduardo C. Lau
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eduardo C. Lau.

Additional information

Capsule Preimplantation HLA matching using tri-, tetra-, and pentanucleotide short tandem repeat (STR) markers in the HLA and flanking sequences for HLA haplotype analysis.

Electronic supplementary materials

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bick, S.L., Bick, D.P., Wells, B.E. et al. Preimplantation HLA haplotyping using tri-, tetra-, and pentanucleotide short tandem repeats for HLA matching. J Assist Reprod Genet 25, 323–331 (2008). https://doi.org/10.1007/s10815-008-9233-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-008-9233-2

Keywords

Search

Navigation