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Dual-mode colorimetric electrochemical aptamer sensor based on Au@Pd bimetallic nanoparticles for the detection of Pb(II) in edible mushrooms

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Abstract

Pb(II) can accumulate in the body and gradually cause irreversible harm. In this work, a dual-mode colorimetric biosensor based on an aptamer was developed as an effective method for sensitive sensing of Pb(II). The aptamers were immobilized on silver nanoparticles (Ag NPs) by Ag–S bonding to increase the active sites on the electrode surface. Gold–palladium bimetallic nanoparticles (Au@Pd NPs) can not only serve as signal indicators but also play a role as nanozymes. On the one hand, Au@Pd NPs were modified on the aptamer to improve the conductivity of the sensor for the detection of Pb(II) by the electrochemical method of differential pulse voltammetry (DPV). On the other hand, Au@Pd NPs were porous nanomaterials with numerous active sites on their surface that can catalyze the decomposition of hydrogen peroxide (H2O2) in solution to generate O2, so that 3,3′,5,5′-tetramethylbenzidine (TMB) was oxidized to blue oxTMB, enabling the visual prediction of Pb(II). At the same time, with the property that 8–17 deoxyribozyme and metal ions can co-catalyze, it promoted the binding of Au@Pd NPs functionalized aptamer to the electrode in the presence of Pb(II) and enhanced the electrochemical signal. Under optimal conditions, the detection range of Pb(II) was 1—1000 nmol L−1, and the limit of detection (LOD) was 0.4 nmol L−1. The biosensor has been successfully applied to the detection of edible mushroom samples.

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References

  1. Zhang J, Barałkiewicz D, Hanć A, Falandysz J, Wang YZ (2020) Contents and health risk assessment of elements in three edible ectomycorrhizal fungi (Boletaceae) from polymetallic soils in Yunnan Province, SW China. Biol Trace Elem Res 195:250–259. https://doi.org/10.1007/s12011-019-01843-y

    Article  CAS  PubMed  Google Scholar 

  2. Kokkoris V, Massas I, Polemis E, Koutrotsios G, Zervakis GI (2019) Accumulation of heavy metals by wild edible mushrooms with respect to soil substrates in the Athens metropolitan area (Greece). Sci Total Environ 685:280–296. https://doi.org/10.1016/j.scitotenv.2019.05.447

    Article  CAS  PubMed  ADS  Google Scholar 

  3. Zhou XS, Gai ZG, Wang YB, Liu SS, Zhang XY, Guo FX, Zhang M, Zhang LL, Jiang X (2023) High performance ratiometric detection towards trace Cd(II) and Pb(II) utilizing in-situ bismuth modified nitrogen rich porous carbon/boron doped diamond composite electrode. J Environ Chem Eng 11(2):109448. https://doi.org/10.1016/j.jece.2023.109448

    Article  CAS  Google Scholar 

  4. Tümay SO, Şenocak A, Sarı E, Şanko V, Durmuş M, Demirbas E (2021) A new perspective for electrochemical determination of parathion and chlorantraniliprole pesticides via carbon nanotube-based thiophene-ferrocene appended hybrid nanosensor. Sens Actuators B Chem 345:130344. https://doi.org/10.1016/j.snb.2021.130344

    Article  CAS  Google Scholar 

  5. Narayanan M, Amalraj A, Perumal P (2023) A copper oxide functionalized neodymium oxide hybrid nanostructure interconnected with modified glassy carbon electrode (CuO–Nd2O3/GCE) for electrochemical determination of Malathion pesticide. J Mater Res 38:416–428. https://doi.org/10.1557/s43578-022-00826-5

    Article  CAS  ADS  Google Scholar 

  6. Narayanan M, Amalraj A, Perumal P (2022) Highly efficient peroxidase-like activity of a metal–oxide-incorporated CeO2-MIL(Fe) metal-organic framework and its application in the colorimetric detection of melamine and mercury ions via induced hydrogen and covalent bonds. Analyst 147:3234–3247. https://doi.org/10.1039/D2AN00864E

    Article  PubMed  Google Scholar 

  7. Huang PP, Chang Q, Jiang GD, Wang X, Zhu HP, Liu QQ (2022) Rapidly and ultra-sensitive colorimetric detection of H2O2 and glucose based on ferrous-metal organic framework with enhanced peroxidase-mimicking activity. Spectrochimica Acta A Mol Biomol Spectrosc 121943:1386–1425. https://doi.org/10.1016/j.saa.2022.121943

    Article  CAS  Google Scholar 

  8. Khawaji M, Chadwick D (2020) Selective oxidation using Au-Pd catalysts: Role of the support in the stabilization of colloidal Au-Pd NPs. Catal Today 348:203–211. https://doi.org/10.1016/j.cattod.2019.08.036

    Article  CAS  Google Scholar 

  9. Komkova MA, Zarochintsev AA, Karyakin AA (2022) Nanozymes ‘artificial peroxidase’ in reduction and detection of organic peroxides. J Electroanal Chem 904:115902. https://doi.org/10.1016/j.jelechem.2021.115902

    Article  CAS  Google Scholar 

  10. Vokhmyanina DV, Andreeva KD, Komkova MA, Karyakina EE, Karyakin AA (2020) ‘Artificial peroxidase’ nanozyme-enzyme based lactate biosensor. Talanta 208:120393. https://doi.org/10.1016/j.talanta.2019.120393

    Article  CAS  PubMed  Google Scholar 

  11. Wang JN, Zeng MQ, Zhao YH, Zuo XX, Meng FR, Jie HY, Lv F, Lu Y, Hou JB (2022) Synergetic integration of catalase and Fe3O4 magnetic nanoparticles with metal organic framework for colorimetric detection of phenol. Environ Res 206:112580. https://doi.org/10.1016/j.envres.2021.112580

    Article  CAS  PubMed  Google Scholar 

  12. Nguyen TTQ, Kim ER, Gu MB (2022) A new cognate aptamer pair-based sandwich-type electrochemical biosensor for sensitive detection of Staphylococcus aureus. Biosens Bioelectron 198:113835. https://doi.org/10.1016/j.bios.2021.113835

    Article  CAS  PubMed  Google Scholar 

  13. Ying X, Li W, Wen Q, Xu D, Ren JL, Lin QL (2021) Aptamer-engineered nanomaterials to aid in mycotoxin determination. Food Control. https://doi.org/10.1016/j.foodcont.2021.108661

    Article  Google Scholar 

  14. Shirani M, Kalantari H, Khodayar MJ, Kouchak M, Rahbar N (2020) A novel strategy for detection of small molecules based on aptamer/gold nanoparticles/graphitic carbon nitride nanosheets as fluorescent biosensor. Talanta 219:121235. https://doi.org/10.1016/j.talanta.2020.121235

    Article  CAS  PubMed  Google Scholar 

  15. Wang RR, Cao Y, Qu H, Wang YB, Zheng L (2022) Label-free detection of Cu(II) in fish using a graphene field-effect transistor gated by structure-switching aptamer probes. Talanta 237:122965. https://doi.org/10.1016/j.talanta.2021.122965

    Article  CAS  PubMed  Google Scholar 

  16. Liang G, Man Y, Li A, Jin XX, Liu XH, Pan LG (2017) DNAzyme-based biosensor for detection of lead ion: a review. Microchem J 131:145–153. https://doi.org/10.1016/j.microc.2016.12.010

    Article  CAS  Google Scholar 

  17. Tan Y, Qiu JZ, Cui MY, Wei XF, Zhao MM, Qiu B, Chen GN (2016) An immobilization free DNAzyme based electrochemical biosensor for lead determination. Analyst 141:1121–1126. https://doi.org/10.1039/C5AN02114F

    Article  CAS  PubMed  ADS  Google Scholar 

  18. Yu YJ, Yu C, Niu YZ, Chen J, Zhao YL, Zhang YC, Gao RF, He JL (2018) Target triggered cleavage effect of DNAzyme: Relying on Pd-Pt alloys functionalized Fe-MOFs for amplified detection of Pb2+. Biosens Bioelectron 101:297–303. https://doi.org/10.1016/j.bios.2017.10.006

    Article  CAS  PubMed  Google Scholar 

  19. Zhou Q, Lin YX, Lin YP, Wei QH, Chen GN, Tang DP (2016) Highly sensitive electrochemical sensing platform for lead ion based on synergetic catalysis of DNAzyme and Au-Pd porous bimetallic nanostructures. Biosens Bioelectron 78:236–243. https://doi.org/10.1016/j.bios.2015.11.055

    Article  CAS  PubMed  Google Scholar 

  20. Song XL, Wang Y, Liu S, Zhang X, Wang JF, Wang HW, Zhang FF, Yu JH, Huang JD (2019) A triply amplified electrochemical lead(II) sensor by using a DNAzyme and via formation of a DNA-gold nanoparticle network induced by a catalytic hairpin assembly. Microchim Acta 186:559. https://doi.org/10.1007/s00604-019-3612-5

    Article  CAS  Google Scholar 

  21. Mandal R, Baranwal A, Srivastava A, Chandra P (2018) Evolving trends in bio/chemical sensor fabrication incorporating bimetallic nanoparticles. Biosens Bioelectron 117:546–561. https://doi.org/10.1016/j.bios.2018.06.039

    Article  CAS  PubMed  Google Scholar 

  22. Sun HY, Chen SJ, Yang WJ, Wang LZ, Tang R, Zhang XM, Zheng RK, Gu SS, Jiang YJ, Liang WB, Huang J (2021) Plasmon-enhanced alcohol oxidations over porous carbon nanosphere-supported palladium and gold bimetallic nanocatalyst. Appl Catal B 292:120151. https://doi.org/10.1016/j.apcatb.2021.120151

    Article  CAS  Google Scholar 

  23. Li SS, Wang AJ, Hu YY, Fang KM, Chen JR, Feng JJ (2014) One-step, seedless wet-chemical synthesis of gold@palladium nanoflowers supported on reduced graphene oxide with enhanced electrocatalytic properties. J Mater Chem A 2:18177–18183. https://doi.org/10.1039/C4TA04164J

    Article  CAS  Google Scholar 

  24. Li RY, Yang TT, Li ZJ, Gu ZG, Wang GL, Liu JK (2017) Synthesis of palladium@gold nanoalloys/nitrogen and sulphur-functionalized multiple graphene aerogel for electrochemical detection of dopamine. Anal Chim Acta 954:43–51. https://doi.org/10.1016/j.aca.2016.12.031

    Article  CAS  PubMed  Google Scholar 

  25. Zhou HF, Chen LM, Li ST, Huang SQ, Sun YY, Chen YH, Wang Z, Liu W, Li XC (2020) One-step electroreduction preparation of multilayered reduced graphene oxide/gold-palladium nanohybrid as a proficient electrocatalyst for development of sensitive hydrazine sensor. J Colloid Interface Sci 566:473–484. https://doi.org/10.1016/j.jcis.2020.01.105

    Article  CAS  PubMed  ADS  Google Scholar 

  26. Velpula S, Beedu S, Rupula K (2021) Bimetallic nanocomposite (Ag-Au, Ag-Pd, Au-Pd) synthesis using gum kondagogu a natural biopolymer and their catalytic potentials in the degradation of 4-nitrophenol. Int J Biol Macromol. https://doi.org/10.1016/j.ijbiomac.2021.08.211

    Article  PubMed  Google Scholar 

  27. Taghdisi SM, Noor MD, Parirokh L, Mohammad R, Khalil A (2016) An electrochemical aptasensor based on gold nanoparticles, thionine and hairpin structure of complementary strand of aptamer for ultrasensitive detection of lead. Sens Actuators B Chem 234:462–469. https://doi.org/10.1016/j.snb.2016.05.017

    Article  CAS  Google Scholar 

  28. Zheng Y, Wang XY, He SQ, Gao ZH, Di Y, Lu KL, Li K, Wang JD (2019) Aptamer-DNA concatamer-quantum dots based electrochemical biosensing strategy for green and ultrasensitive detection of tumor cells via mercury-free anodic stripping voltammetry. Biosens Bioelectron 126:261–268. https://doi.org/10.1016/j.bios.2018.09.076

    Article  CAS  PubMed  Google Scholar 

  29. Wang LY, Peng XL, Fu HJ (2022) An electrochemical aptasensor for the sensitive detection of Pb2+ based on a chitosan/reduced graphene oxide/titanium dioxide. Microchem J 174:106977. https://doi.org/10.1016/j.microc.2021.106977

    Article  CAS  Google Scholar 

  30. Ma JP, Bai WS, Zheng JB (2022) A novel self-cleaning electrochemical biosensor integrating copper porphyrin-derived metal-organic framework nanofilms, G-quadruplex, and DNA nanomotors for achieving cyclic detection of lead ions. Biosens Bioelectron 197:113801. https://doi.org/10.1016/j.bios.2021.113801

    Article  CAS  PubMed  Google Scholar 

  31. Fu T, Ren SL, Gong L, Meng HM, Cui L, Kong RM, Zhang XB, Tan WH (2016) A label-free DNAzyme fluorescence biosensor for amplified detection of Pb2+-based on cleavage-induced G-quadruplex formation. Talanta 147:302–306. https://doi.org/10.1016/j.talanta.2015.10.004

    Article  CAS  PubMed  Google Scholar 

  32. Zhang Q, Cai Y, Li H, Kong DM, Shen HX (2012) Sensitive dual DNAzymes-based sensors designed by grafting self-blocked G-quadruplex DNAzymes to the substrates of metal ion-triggered DNA/RNA-cleaving DNAzymes. Biosens Bioelectron 38:331–336. https://doi.org/10.1016/j.bios.2012.06.011

    Article  CAS  PubMed  Google Scholar 

  33. Zhu Q, Liu LH, Xing YP, Zhou XH (2018) Duplex functional G-quadruplex/NMM fluorescent probe for label-free detection of lead(II) and mercury(II) ions. J Hazard Mater 355:50–55. https://doi.org/10.1016/j.jhazmat.2018.04.082

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the Natural Science Foundation-PhD of Shandong Province (ZR2018BC055) for research on the mechanism and analysis method of biosensor based Pb(II) detection in edible mushrooms.

Funding

Natural Science Foundation of Shandong Province, ZR2018BC055.

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Authors and Affiliations

  1. School of Agricultural Engineering and Food Science, Shandong University of Technology, No.266 Xincun Xilu, Zibo, 255049, Shandong, China

    Qi Guo, Jiaqi Yin, Yuhao Zhang, Yue Wang, Hongguo Zhai, Luting Yan, Meijia Tian, Yemin Guo, Xia Sun & Yanyan Zhang

  2. Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Beijing, 100097, China

    Qi Guo

  3. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No.266 Xincun Xilu, Zibo, 255049, Shandong, China

    Yemin Guo, Xia Sun & Yanyan Zhang

  4. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No.266 Xincun Xilu, Zibo, 255049, Shandong, China

    Yemin Guo, Xia Sun & Yanyan Zhang

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  1. Qi Guo
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  2. Jiaqi Yin
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  9. Xia Sun
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  10. Yanyan Zhang
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Contributions

QG: Conceptualization, Methodology, Writing-original draft. JY: Methodology, Writing-review & editing. YZ: Investigation, Writing-review & editing. YW: Validation. HZ: Validation. LY: Validation, Investigation. MT: Resources, Writing-review & editing. XS: Formal analysis. YG: Formal analysis. YZ: Supervision, Funding acquisition.

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Correspondence to Yanyan Zhang.

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Guo, Q., Yin, J., Zhang, Y. et al. Dual-mode colorimetric electrochemical aptamer sensor based on Au@Pd bimetallic nanoparticles for the detection of Pb(II) in edible mushrooms. J Appl Electrochem (2024). https://doi.org/10.1007/s10800-024-02070-7

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