Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients

Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients. The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewisy and Lewisx structures, respectively. However, a correlation between IgG core fucosylation and IgG concentration (r = −0.52; p < 0.0003) was observed. The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002). In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa. The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group. In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands. Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively). The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.


Introduction
From an immunological point of view, seminal plasma is designed to protect male sperm cells against infection and to inhibit the immune response against sperm cells in both male and female reproductive organs. Seminal plasma has inhibitory properties due to the presence of biologically active factors, such as enzymes, steroids, hormones, proteins, cytokines and immunoglobulins, mainly G (IgG) and A (IgA).
Physiologically, normal human ejaculate does not contain more than 1×10 6 /ml of leukocytes, but in leukocytospermia the number of leukocytes exceeds this value [1]. Leukocytes play an important role in the immune "supervision" [2] and removal of abnormal sperm cells by phagocytosis [3]. The increased number of leukocytes in semen indicates urogenital tract infection and inflammation [4]. Adversely, leukocytes are a source of oxidative stress [5] and thus lower the quality of male gametes [6]. Leukocytospermia is frequently observed in 10-20 % of subfertile and 10-44 % of infertile men [7,8]. Yilmaz et al. [9] showed that the amount of sperm and number of sperm cells with fast progressive movement in leukocytospermic men is two times lower than those observed in patients without leukocytospermia with abnormal sperm parameters.
Immunoglobulin G, the most abundant immunoglobulin in blood plasma, is involved in the recognition, neutralization and elimination of pathogens and toxic antigens. Glycans attached to blood plasma IgG molecules may differ in the content of fucose, galactose, sialic acid and presence or absence of bisecting N -acetylglucosamine [10]. An important role is attributed to the presence of core fucose α(1-6)linked on Nglycans of IgG [11]: its absence causes a 50-fold increase in antibody binding to the FcγRIIIA present on natural killer cells [12], thus increasing antibody or complement-mediated cellular cytotoxicity [13]. Little is known about the profile of human seminal plasma IgG fucosylation.
Secretory immunoglobulin A (S-IgA), accounts for two thirds of antibodies found on mucosal surfaces and is present in colostrum, tears, sweat, gastrointestinal secretions, respiratory and urogenital tracts [14,15]. Secretory IgA prevents microorganisms and other antigens from penetrating the surface of the mucous membranes. It is also responsible for neutralization of viruses and enhances non-specific defence mechanisms [16]. Glycans of S-IgA form additional binding sites for bacteria and may participate in both the innate and acquired immunity [17]. The most important feature of S-IgA is the presence of a secretory component (SC) [18], which is a glycoprotein of 50-90 kDa [14]. The secretory component is synthesized by epithelial cells of the digestive system, respiratory and urogenital tracts. SC may be attached to immunoglobulin A or M, and/or may exist in free form in secretions. The secretory module gives S-IgA structural stability and increases its resistance to proteolytic digestion during transportation through the epithelium to the mucous-serous secretions [19]. Secretory component contains 5-7 N-linked oligosaccharides, which constitute about 22 % of its total molecular mass [20]. Royle et al. [17] in their studies on human colostrum S-IgA, showed that most of the oligosaccharide structures expressed on human SC are bi-antennary. Tri-and tetra-antennary glycans are less abundant (11.7 % and <1 %, respectively). All the glycans contain galactose, but not bisecting GlcNAc [17]. Most N-glycans (70 %) expressed on SC contain sialic acid, and over 65 % of the glycans contain core fucose. Some of the oligosaccharide antennas may also be fucosylated. The secretory molecule may express each of the known structures of Lewis-and/or sialyl-Lewis-types, which are responsible for specific binding of bacterial adhesins [17].
Our previous study [21] showed that seminal S-IgA concentration is not associated with sperm parameters. Immunoblotting analysis has shown that seminal SC is present in two forms, with molecular masses around 80-and 60-kDa [21]. In the present study we investigated the differences in fucosylation of seminal IgG and IgA secretory component forms and their association with leukocytospermia of infertile males.

Specimens
Human ejaculates were collected from fertile donors (26-45 years old) and leukocytospermic infertile patients (age matched to the normal group). The ejaculates were collected by masturbation into sterile containers after 3-5 days of sexual abstinence, and were allowed to stand at 37°C until liquefaction (no longer than 1 h). Next, standard semen analysis (volume, pH, morphology, sperm concentration, motility, and viability) was done at the Warsaw InviMed semen analysis laboratory according to WHO criteria [1]. Semen samples were centrifuged at 3500 × g for 10 min at room temperature to obtain plasma. Seminal plasma was divided into small aliquots and frozen at −76°C until use. Ejaculates were collected according to ethical standards (Ethical Committee approval KB-216/2011).
Seminal plasma samples were divided into two groups: normal (n =17) and leukocytospermic (n =28). In the normal group, the count of spermatozoa was higher than 15×10 6 /ml and more than 4 % expressed the correct sperm morphology with a total motility of ≥40 % or progressive motility ≥32 % at 1 h after ejaculation. The leukocytospermic group was formed from samples in which the leukocyte number was higher than 1×10 6 /ml according to WHO criteria [1]. None of the leukocytospermic samples were normozoospermic.
IgG and S-IgA concentration Immunoglobulin G concentration was determined by radial immunodiffusion [22] using goat anti-human IgG polyclonal antibodies (BIOMED, Warsaw, Poland) and Human Serum Protein Calibrator (DakoCytomation, Denmark) as a standard. The concentration of S-IgA was determined by sandwich ELISA, using mouse monoclonal antibody directed against human SC of IgA (1:20,000; Sigma Chemical Co., St. Louis, MO, USA) and preparation of IgA from human colostrum (Sigma Chemical Co., St. Louis, MO, USA) as a standard. Rabbit anti-human IgA polyclonal antibodies (1:100,000; DakoCytomation, Denmark) were used to quantify the amount of seminal S-IgA bound by the capture antibody. Goat anti-rabbit IgG-HRP (1:10,000; Sigma Chemical Co., St. Louis, MO, USA) was used as a detection antibody. O -phenylenediamine dihydrochloride activated with H 2 O 2 was used as the enzyme substrate. Colorimetric reaction was subsequently stopped with 12.5 % H 2 SO 4 . The colour intensity was measured in a Stat Fax 2100 Microplate Reader (Awareness Technology Inc., Palm City, FL, USA) at 492 nm using a reference filter at 630 nm. All ELISA immunobinding reactions and washing steps were carried out in 10 mM Trisbuffered saline (TBS) containing 0.05 % Tween 20, pH 7.5, and the blocking step in the presence of 0.1 % BSA (Sigma Chemical Co., St. Louis, MO, USA). Background absorbance (lower than 0.1 AU) was measured for TBS containing 0.05 % Tween 20, pH 7.5 instead of a seminal plasma sample, but with all other reagents. Seminal plasmas with known concentrations of S-IgA were used as positive controls. All samples were analysed in duplicate.

Removal of terminal sugars from capture antibodies
Monoclonal anti-human IgG antibodies had to be defucosylated before use in lectin-ELISA to avoid the lectin binding to capture antibodies. We have previously described the IgG defucosylation procedure [23]. Briefly, one volume of polyclonal goat anti-human IgG antibodies (200 μl, pH=8.1) was mixed with an equal volume of 100 mmol/l NaIO 4 in 100 mmol/l NaHCO 3 , 0.2 % Tween 20, pH 8.1. The mixture was incubated for 90 min. at room temperature in the dark and subsequently dialysed against 100 mmol/l NaHCO 3 , pH 9.2, for 3 h at 4°C. Such treatment resulted in elimination of antibody reactivity with fucose-specific lectins.
Lectin-ELISA procedure Expression of exposed fucosyl-residues of glycoproteins was determined by fucose-specific lectins AAL, UEA and LTA, as described earlier [23]. Defucosylated polyclonal goat antihuman IgG antibodies (BIOMED, Warsaw, Poland) were diluted in 10 mM TBS pH 8.5 (1:10,000), coupled to a polystyrene microtiter ELISA plate and incubated for 2 h at 37°C. Seminal plasma samples were diluted in 10 mM TBS, 1 mM CaCl 2 , 1 mM MgCl 2 , 0.05 % Tween 20, and 0.5 % glycerol, pH 7.5, to obtain a glycoprotein solution containing 100 ng of IgG in 100 μl. Upon addition of seminal plasma samples, plates were incubated for 2 h at 37°C. All samples were analysed in duplicate. Background absorbance was measured for samples in which all reagents were present, but seminal plasma was replaced with a 10 mM TBS, 1 mM CaCl 2 , 1 mM MgCl 2 , 0.05 % Tween 20, and 0.5 % glycerol, pH 7.5. To control for the specificity of lectin-glycoprotein interaction and to check the absence of detectable endogenous reactive materials, control probes were included for the test. Haptoglobin and asialohaptoglobin preparations derived from ovarian cancer fluid were used as positive controls [28], whereas a human albumin preparation served as negative control (Sigma Chemical Co., St. Louis, MO, USA). The α(1-6)-, α(1-3)and α(1-2)linked fucose residues in IgG were detected by biotinylated AAL, LTA and UEA, respectively. Lectin dilutions (1:7500, 1:100 and 1:250, respectively) were established in preliminary experiments. All lectins were diluted in 10 mM TBS containing 1 mM CaCl 2 , 1 mM MgCl 2 , 0.05 % Tween 20, and 0.5 % glycerine, pH 7.5, and the plate was incubated for 1 h at 37°C. The formed complex of IgG-biotinylated lectin was quantitated using phosphatase-labelled ExtrAvidin (1 h at 37°C; 1:20,000; Sigma Chemical Co., St. Louis, MO, USA) and detected by the reaction with di-sodium 4-nitrophenyl phosphate (Merck, Darmstadt, Germany). The results were expressed in absorbance units (AU) measured at 405 nm with a reference filter of 630 nm in ELISA Stat Fax 2100 Microplate Reader (Awareness Technology Inc., Palm City, FL, USA). To remove any excess protein, the plate was washed three times with 10 mM TBS, 0.05 % Tween 20, pH=7.5 between each ELISA-step. The background absorbance values were not higher than 0.2 AU.

Determination of SC bands by immunoblotting
To determine the most characteristic immunoblot pattern for SC in leukocytospermic and normal seminal groups, equal volumes of samples in each seminal group were pooled. Next, the samples were diluted with 0.06 M Tris-HCl, pH=6.8 containing 3 % SDS (w/v), 10 % glycerol (v/v) and 7.5 % 2-mercaptoethanol (v/v), and boiled at 100°C for 5 min. Then 250 ng of S-IgA, determined by ELISA (for details see S-IgA concentration determination) was subjected to SDSpolyacrylamide gel electrophoresis on a 10 % gel according to Laemmli [29] in a BioRad-vertical system (BioRad, Richmond, CA, USA). The separated proteins were subsequently blotted onto nitrocellulose (Serva Electrophoresis GmbH, Heidelberg, Germany). After blocking (3 % powdered skim milk in 50 mM TBS, pH 7.5), the blots were incubated with mouse monoclonal antibodies anti-human SC of IgA diluted at 1:1000 in 3 % powdered skim milk in 50 mM TBS, pH 7.5, and probed with goat anti-mouse IgG polyclonal antibodies conjugated with horseradish peroxidase (1:2000 dilution in 3 % powdered skim milk dissolved in 50 mM TBS; Sigma Chemical Co., St. Louis, MO, USA). The colour reaction was developed with diaminobenzidine and H 2 O 2 . After each step, the nitrocellulose was washed at least 3 times using 50 mM TBS, 0.05 % Tween; pH=7.5. Finally, the blots were dried and analysed. The blots were digitised and bands corresponding to secretory component of IgA (SC) were analysed with densitometry ImageJ 1.42q gel analysis software (National Institutes of Health, U.S. Department of Health & Human Services public domain). The relative amounts of particular SC bands were expressed as the percentage of the total number of pixels in a lane. To calculate the molecular masses of SC bands, low molecular-weight protein mass standard (97.4-6.5 kDa; Serva Electrophoresis GmbH, Heidelberg, Germany) and human colostrum S-IgA preparation (Sigma Chemical Co., St. Louis, MO, USA) were used.
Lectin-blotting determination of secretory component fucose expression The relative reactivity of SC bands was analysed in lectinblotting with biotinylated fucose-specific lectins, according to the procedure described earlier by Kratz et al. [30] for synovial fluid IgG and IgA. For lectin-binding analysis only those bands that corresponded to SC detected in immunoblotting with mouse anti-human SC of IgA monoclonal antibodies were taken into consideration (see the section above). Nitrocellulose membranes were labelled with AAL (1:400), LTA (1:200) and UEA (1:200) (Vector Laboratories Inc., Burlingame, CA, USA) diluted in 3 % powdered skim milk in 50 mM TBS pH=7.5, and then detected with alkaline phosphatase-conjugated ExtrAvidin (1:10,000 dilution in 3 % milk in 50 mM TBS pH=7.5; Sigma Chemical Co., St. Louis, MO, USA). The colour reaction was developed with 0.1 M Tris-HCl; 0.1 M NaCl; 0.05 M MgCl 2 , pH=9.5 containing 100 μl 7.7 % nitro blue tetrazolium in dimethylformamide (DMF) (7:3; v/v), and 75 μl 5 % 5-bromo-4chloro-3-indolyl phosphate, disodium salt in DMF. Lastly, the blots were dried and analysed. Bands corresponding to particular AAL-, LTA-and UEA-reactive SC were digitised and analysed with densitometry ImageJ 1.42q gel analysis software (National Institutes of Health, U.S. Department of Health & Human Services public domain). The relative amounts of particular fucose-specific SC bands were expressed as the percentage of the total number of pixels in a lane. As described previously, to calculate the molecular masses of SC bands, low molecular-weight protein mass standard (Serva Electrophoresis GmbH, Heidelberg, Germany) and human colostrum S-IgA preparation (Sigma Chemical Co., St. Louis, MO, USA) were used. Specific lectin reactivity reflecting the density of fucosylated epitopes [31,32] was defined as the ratio of lectin reactivity to the protein content, measured as reactivity with anti-human SC of IgA in a particular band, and calculated as: Lectin reactivity (pixels/band)/ Antibody reactivity (pixels/band). Specific lectin reactivity was used to compare relative fucosylation of both SC forms ( Table 2).

Statistical analysis
Statistical analysis was performed using STATISTICA 10.0 software (StatSoft Inc., Tulsa, OK, USA). Experimental data were presented as means and standard deviations (SD), and distribution of the values within analysed groups as box-whisker plots with median and interquartile (25 th -75 th percentile) range. According to a Shapiro-Wilk W test, the values did not fit normal distribution, thus the nonparametric Mann-Whitney U test was used to determine differences between groups. Correlations between determined parameters were estimated according to a Spearman test. A two-tailed p-value of less than 0.05 was considered significant.

Concentration of IgG and secretory IgA
IgG concentration in leukocytospermic seminal plasmas was significantly higher than that observed in normal seminal plasma (131.7±103 mg/l and 67.7±29 mg/l, respectively; p <0.02; Table 1). Also S-IgA concentration was significantly higher in the leukocytospermic group compared to normal seminal plasmas (36±27 mg/l and 19±18 mg/l, respectively; p <0.02; Table 2). The median value of IgG concentration was 104 and 63.3 mg/l, and 28.7 and 9.9 mg/l for S-IgA in leukocytospermic and normal seminal groups, respectively (Fig. 1a, b).

IgG reactivity with lectins
The relative reactivity of IgG with AAL was significantly lower in the leukocytospermic seminal group (0.82±0.3 AU,  Fig. 3), although the difference did not reach statistical significance (p >0.05).

SC reactivity with monoclonal antibody
In the pools of leukocytospermic and normal seminal plasma groups the presence of 78±4.2-kDa and 63±2.3-kDa SC bands were observed in SDS-PAGE under reducing conditions and subsequent immunoblotting with mouse anti-human SC of IgA monoclonal antibody (Fig. 4). Both SC bands may represent free secretory component and/or a fraction of S-IgA (S-IgA1 and/or S-IgA2) released after S-IgA reduction during the SDS-PAGE procedure. Their relative content was different in both seminal groups. The 78-kDa SC in leukocytospermic seminal plasmas was expressed at 77.5 %, and in the normal group at 83.5 %, and as a consequence the percentage participation of 63-kDa SC in the leukocytospermic group was higher (22.5 %) than in the normal seminal group (16.5 %) ( Table 2). The concentration of S-IgA in seminal plasmas was determined by immunoenzymatic sandwich ELISA test, using mouse anti-human SC of IgA monoclonal antibodies. Results are given as mean value ± standard deviation. A two-tailed p-value of less than 0.05 was considered significant. Two SC bands were determined by immunoblotting using anti-human SC of IgA monoclonal antibody: 78±4.2 kDa and 63±2.3 kDa (see Fig. 4). On the basis of densitometric analysis, the relative content of particular SC bands was expressed as the percentage of the total number of pixels in a lane (for details see Methods section). The reactivity of SC with biotinylated fucose-specific lectins AAL, UEA and LTA was analysed by densitometry as previously described [30]. The relative content of particular fucose-specific SC bands, corresponding to SC bands reactive with anti-human SC of IgA antibodies, was expressed as the percentage of the total number of pixels in a lane a specific lectin reactivity, defined as a ratio of lectin and antibody reactivity (for details see Methods section) Fig. 1 Concentration of IgG and S-IgA. The concentrations of IgG (a) and S-IgA (b) in seminal plasmas were determined by radial immunodiffusion [22] and immunoenzymatic sandwich ELISA test, using goat anti-human IgG polyclonal antibodies and mouse anti-human SC of IgA monoclonal antibodies, respectively SC reactivity with lectins The lectin-binding patterns of SC with fucose-specific lectins are shown in Fig. 5. In the leukocytospermic group, the relative amount of AAL-and LTA-reactive 78-kDa SC bands was visibly higher (66 % and 73.1 %, respectively) than those observed for the normal group (56.8 % and 66.3 %, respectively) ( Table 2). The relative amount of UEA-reactive 78-kDa SC band was lower in the leukocytospermic group in comparison to normal seminal plasma (68.4 % and 80.4 %, respectively; Table 2). Despite the fact that 63-kDa fraction accounted for 16.5 % (normal group) and 22.5 % (leukocytospermia) of total SC (Table 2), its contribution in lectin reactivity reached 43 % (relative amount of AAL-reactive 63-kDa SC in the normal group) and in all cases was higher than the relative protein content shown in Table 2. This may suggest that fucosylation of low molecular weight 63-kDa SC was higher than that observed for the 78-kDa fraction. To confirm this suggestion, specific lectin reactivity was calculated, defined as a ratio of lectin reactivity to the protein content in each particular band (see Methods section and Table 2). Specific lectin reactivity of 63-kDa SC with AAL was about 3.6 times higher than that observed for 78-kDa SC in the normal seminal group (6.9 and 1.9, respectively), but similar in leukocytospermia (2.7 and Fig. 2 Dependence between IgG concentration and IgG relative reactivity with lectins. Correlation between IgG concentration and IgG relative reactivity with AAL (a), and UEA (b) was estimated according to a Spearman test, with a two-tailed p-value of less than 0.05 considered significant. 95 % confidence interval is marked by dotted lines Fig. 3 IgG relative reactivity with fucose-specific lectins. The relative reactivity of seminal IgG with biotinylated fucose-specific lectins AAL, UEA and LTA was determined in normal and leukocytospermic groups using lectin-ELISA [23] and expressed in absorbance units (AU). □median value Fig. 4 Representative immunoblotting pattern of seminal SC. 250 ng of S-IgA determined by ELISA (for details see Methods section), was subjected to SDS-PAGE on 10 % gel according to Laemmli [29]. Immunoblots were probed with mouse anti-human SC of IgA monoclonal antibody. Lane S -low molecular-weight protein mass standard; Lane 1preparation of S-IgA from human colostrum; Lane 2 -normal seminal plasma; Lane 3 -leukocytospermic seminal plasma 2.3, respectively; Table 2). Also the specific lectin reactivity of 63-kDa SC with LTA was about 2.5 times higher than that observed for 78-kDa SC (5.1 and 2.1, respectively) in the normal group. However, no changes between specific lectin reactivity with LTA for both SC bands were observed in leukocytospermia ( Table 2). The differences between the specific lectin reactivity with UEA of both SC forms in the normal and leukocytospermic groups were negligible ( Table 2).

Discussion
Our present work, for the first time, shows the profile and degree of human seminal IgG and SC fucosylation. The following findings emerge from our studies: (1) in leukocytospermic seminal plasmas, the mean concentrations of IgG and S-IgA are twice as high as in the normal group, (2) the seminal IgG and SC are decorated with core fucose, as well as antennary UEA-and LTA-reactive fucose of Lewis y and Lewis x structures, (3) the IgG present in leukocytospermic samples is characterized by lower expression of AAL-reactive core fucose than in the normal group, (4) in human seminal plasma the SC is present in two forms: 78-kDa and 63-kDa, differing in their fucose expression, (5) in the normal seminal group, higher AAL and LTA specific reactivity of 63-kDa SC glycans is observed as opposed to 78-kDa SC, (6) in leukocytospermia the values of specific lectin reactivity for core and Lewis x fucose of 63-kDa SC were lower but similar for both SC forms.
In all the glycosylation studies based on the application of lectins, the results shouldn't be regarded as reflecting the accurate structure of the oligosaccharides. Lectins may be able to bind less preferred structures when they are abundant enough, but also not bind the glycans which are conformationally hidden in the glycoprotein structure. In spite of this drawback, lectin reactivity reflects the real accessibility of the analysed glycoepitopes in the native microenvironment, thus also their potential for interactions in vivo.
It is suggested that the content of L-fucose in the glycan moiety of seminal plasma glycoproteins, as well as fucose location in these structures, may influence various interactions during sperm maturation and fertilization cascade. Seminal plasma oligosaccharides containing fucose of Lewis x and/or Lewis y glycotopes, both as free oligosaccharides and attached to glycoconjugates, participate in sperm-egg binding initiating the fertilization process [33], and has been reported to interfere with gamete fusion [34]. Fucosylation of immunoglobulins may be of key importance due to their participation in the immune response.
Concentrations of seminal IgG determined in the normal group (Table 1) corresponded to the values reported earlier [35,36]. The slightly higher S-IgA concentrations previously reported [21] may result from different characteristics of normozoospermic groups. In the previous study, normozoospermic semen samples were collected from men living in infertile couples, while the group analysed here is composed of apparently fertile men. In leukocytospermic seminal plasma, the concentration of both IgG and S-IgA was significantly increased, and this may be due to inflammation of the male reproductive organs of various aetiology. The presence of antisperm IgG antibodies may be also possible. High immunoglobulin level and increased defence reactions may, however, be harmful for fertility potential. For example the speed of sperm penetration through the cervical mucus and fertilization effectiveness were reduced when at least 50 % of motile sperm were bound to IgG antibodies [37].
Changes in α(1-6) core fucosylation of IgG glycans are associated with the effector functions of immunoglobulin G [12,13]. Lack of the core fucose on IgG glycans, resulting from the decreased activity of α1,6-fucosyltransferase [38], or the presence of bisecting GlcNAc [39], may contribute to defence mechanisms [35], through increased cytotoxicity of NK cells and monocytes [40]. The changes in the expression of immunoglobulin G core fucosylation were observed in some diseases including rheumatoid arthritis [30,41,42] and different types of cancer: ovarian [43], stomach [44] and liver [45]. Core fucose is also known to protect N-glycans via inhibition of their hydrolysis with glycoasparaginases [46]. On the other hand, fucose located in the antennary part of glycans of Lewis y and Lewis x -/sialyl-Lewis x antigens is known to participate in cellular recognition. Antennary fucose of Lewis x and Lewis y types may be engaged in binding and inactivation of bacteria through interaction with bacterial adhesins [17]. The sperm surface was shown to contain numerous receptors for simple and complex sugars [47][48][49][50][51]. Incubation of the male gametes with fucoidin inhibits the binding of sperm to the zona pellucida of the oocyte [52,53]. Therefore, antennary fucose α(1-2)linked, present in glycans of seminal plasma glycoproteins [54], and bifucosylated Lewis y oligosaccharide structures can participate in adhesion in the maternal-fetal interactions during embryo implantation [55,56].
In our study we have found a significant decrease of IgG AAL-reactivity and a negative correlation of this core fucose Fig. 5 Relative reactivity of SC with fucose-specific lectins. Lectinblotting with three biotinylated fucose-specific lectins AAL, UEA and LTA [30] was performed for normal (1) and leukocytospermic (2) seminal groups. The bands corresponding to SC (78±4.2 kDa and 63± 2.3 kDa) are shown by arrows expression with IgG concentration in seminal plasma. This may elevate effector interactions of IgG and cytotoxicity of NK in leukocytospermic patients, which may also result in some damage of sperm cells. The differences in IgG UEA-and LTA-reactivity between normal and leukocytospermic groups are slight and statistically insignificant. However, comparing the reactivity of seminal plasma IgG with all three fucosespecific lectins, it is noteworthy that in leukocytospermic IgG there is a shift in fucose location from the core to antennary part, making an increase in Lewis x possible. Termination of glycans with sialic acid is known to limit binding of LTA [27]. Recently, similar observations were also made for core-fucose specific AAL [57,58]. On the other hand, the presence of α(2-3)linked sialic acid prevents formation of bifucosylated, UEA-reactive Lewis y antigens [26]. In IgG, the accessibility of fucose may also be influenced by conformational changes hiding the glycans inside an Fc structure [58]. Thus, the results presented here reflect the accessibility of glycans for the ligands differing in their specificity. To obtain more information on the real chemical structure of the oligosaccharides, further sialylation and structural studies are planned.
Secretory component of IgA is capable of reacting via its glycans with bacterial adhesins leading to inactivation and prevention of infection by bacterial microorganisms [59]. In human secretions, SC is reported to exist in both free and IgA bound forms. It is not clear, however, if there is any difference in their structure and function [14,60]. In seminal plasma, as well as in human colostrum, two forms of secretory component were found, differing in their molecular masses. The values are reported as 80-90 kDa for the bigger, and 50-65 kDa for the smaller form [14,21,60]. In the present study molecular masses determined in the immunoblotting analysis of seminal plasma with specific anti-human SC of IgA monoclonal antibody, were 78±4.2 kDa and 63±2.3 kDa (Table 2; Fig. 4). The origin or structural difference between these two forms is still not clear. Almogren et al. [60] have suggested that the 62-kDa SC is a deglycosylated form of 80-kDa SC. Although different glycosylation profiles of the two seminal SC bands observed by us are possible, both of them were capable of binding lectins, thus contain glycan moiety. The smaller form may also result from partial degradation of the 78-kDa SC form [60]. It also cannot be excluded that both fractions represent free secretory component and a fraction bound to IgA as part of S-IgA (S-IgA1 and/or S-IgA2) present in native seminal plasma and released after S-IgA reduction during the procedure of SDS-PAGE.
Comparison of the relative content of both fractions in normal and leukocytospermic seminal plasmas indicates increased expression of 63-kDa SC associated with leukocytospermia (22.5 % for the leukocytospermic and 16.5 % for the normal group; Table 2). If partial deglycosylation or degradation is the mechanism of the formation of the 63-kDa SC form, its increased content may cause the debilitation of its biological antibacterial function. This in turn may reduce the ability of SC to defend sperm against infection and thus influence fertility. SC is reported to contain as many as 5-7 N-linked oligosaccharides of different structures, containing Lewis a , Lewis x and Lewis y structures. The main role of glycans is to protect the SC and S-IgA against proteases, but the glycans are also responsible for the binding of lectins and bacterial adhesins [17]. Alterations in the glycosylation profile probably influence the biological functions of S-IgA and SC as well [17].
Fucosylation of SC was examined by means of lectinblotting, which, as opposed to ELISA tests, enables comparison of reactivity with fucose-specific lectins for both SC forms. Our experiments indicate that the relative reactivity of both SC fractions with the three fucose-specific lectins differ between the normal and leukocytospermic groups. The relative content of the lectin-reactive 63-kDa SC form (19.6-43.2 %) for all three lectins in both groups exceeds its protein content (16.5-22.5 %). It could be hypothesised that fucose expression in the glycans of the 63-kDa form is stronger than in 78-kDa SC. To evaluate this hypothesis, specific lectin reactivity was estimated, defined as a ratio of lectin reactivity to protein content in both forms ( Table 2, values with a). Such an attempt enables comparison of the fucose content extrapolated to the protein amount and reflects the density of fucosylated epitopes on the protein surface [31,32]. In the group with normal semen, the density of AAL-reactive fucosylated epitopes was 3.5-times higher in 63-kDa than in the 78-kDa fraction. Similarly, the density of LTA-reactive fucose was 2.5-times higher in the smaller SC form. In leukocytospermia, the values were comparable for all three lectins. Also the density of the recognized epitopes was similar for all three lectins and both groups in the 78-kDa SC form. The leukocytospermic 63-kDa SC form thus appears to express lower density LTA-and AAL-reactive fucose corresponding to Lewis x antigen and core fucose. This may result in the altered ability of the 63-kDa SC form to interact with bacteria and disturb its defence function. Further examination of the origin and structural difference of the two forms of secretory component as well as their glycosylation are demanded to explain if such changes may be important for the defence molecular mechanism.
In conclusion, the analysis of IgG and SC fucosylation in seminal plasma is an indicator for some disease-related alterations. If an analysis of the sensitivity and specificity of changes reported here will also be satisfactory, these glycosylation traits might constitute valuable diagnostic tools for the evaluation of infertility with accompanying leukocytospermia. Additionally, such information may be helpful as a parameter for selecting the appropriate assisted reproduction technique to be used in leukocytospermic patients. However, larger studies are needed to explore the role of seminal IgG and SC fucosylation profiles in male fertility. Moreover, the fucosylation of seminal IgA molecules might also be interesting, especially in the context of male fertility. Our study did not show the exact glycan structures, but reflects the exposition of the conformationally accessible fucose of seminal IgG and SC for external and internal ligands. Further studies focused on the explanation of the origin and structural differences between two SC forms present in seminal plasma are required because the different fucose expression may be of significant importance for the appropriate function of both free and bound SC forms. Our results suggest that the decrease of both core and Lewis x type fucose content in the 63-kDa SC form in leukocytospermic seminal plasmas may influence the effectiveness of SC in its defence against internal infections of the male reproductive tract.