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Hepatoprotective effects of a Chinese herbal formulation, Yingchen decoction, on olaquindox-induced hepatopancreas injury in Jian carp (Cyprinus carpio var. Jian)

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Abstract

In order to identify effective hepatoprotective herbs for clinical application in fish farming, 200 mg/kg olaquindox (OLA) was added to a basal diet (group 1, control) to form OLA diet (group 2), then 1.35, 2.7 and 5.4 % (w/w) of a Chinese herbal formulation, Yingchen decoction (YCD), were added to the OLA diet to form three additional diets for groups 3, 4 and 5, respectively. A total of 375 juvenile Jian carp (Cyprinus carpio var. Jian) (52.12 ± 2.95 g/tail) were divided into five groups (triplicates per group) and fed the five diets mentioned above, respectively, for 6 weeks. At the termination of feeding experiment, serum biochemical indexes, viability of hepatocytes and the hepatopancreas microstructure for each group were detected and observed. The results showed that serum ALT and AST in group 2 were significantly higher than the control (P < 0.05). Plasma membranes hepatocyte nuclei in group 2 were found to be mostly indistinct, compared to group 1, and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The viability of isolated hepatocytes in group 2 was the lowest and gradually recovered with the increasing supplementation of YCD in group 3, 4 and 5. The results suggest that YCD protected the Jian carp hepatopancreas against injury from OLA, and that 5.4 % YCD would be the optimum dosage in a Jian carp diet.

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References

  • Abdel-Hameed E-SS, Bazaid SA, Sabra ANA (2013) Protective effect of conocarpus erectus extracts on CCl4-induced chronic liver injury in mice. Glob J Pharmacol 7:52–60

    Google Scholar 

  • Chen PF, Chen J, Guo ZX (2006) Preliminary research on the effect of Si-Ni-San on carp’s liver protection and recovery. Feed Ind 27(6):28–31 (in Chinese)

    Google Scholar 

  • Choi WM, Mo WY, Wu SC, Mak NK, Bian ZX, Nie XP, Wong MH (2013) Effects of traditional Chinese medicines (TCM) on the immune response of grass carp (Ctenopharyngodon idellus). Aquac Int. doi:10.1007/s10499-013-9644-7

    Google Scholar 

  • Citarasu T (2010) Herbal biomedicines: a new opportunity for aquaculture industry. Aquac Int 18:403–414

    Article  Google Scholar 

  • De la Torre FR, Salibia’n A, Ferrari L (2007) Assessment of the pollution impact on biomarkers of effect of a freshwater fish. Chemosphere 68:1582–1590

    Article  PubMed  Google Scholar 

  • Di L, Liu XY, Chang HF, Wang YS, Zhang QG (2007) Protective effect of Peony and Licorice decoction on acute liver injury in mice. Pharmacol Clin Chin Mater Med 23:4–5

    Google Scholar 

  • Dvorák Z, Kosina P, Walterová D, Simánek V, Bachleda P, Ulrichová J (2003) Primary cultures of human hepatocytes as a tool in cytotoxicity studies: cell protection against model toxins by flavonolignans obtained from Silybum marianum. Toxicol Lett 137(3):201–212

    Article  PubMed  Google Scholar 

  • Elferink RO (2004) Yin Zhi Huang and other plant-derived preparations: where herbal and molecular medicine meet. J Hepatol 41:691–693

    Article  PubMed  Google Scholar 

  • Han KH, Jeon YJ, Athukorala Y, Choi KD, Kim CJ, Cho JK et al (2006) A water extract of Artemisia capillaries prevents 2,2′-azobis(2-amidinopropane) dihydrochloride-induced liver damage in rats. J Med Food 9:342–347

    Article  PubMed  Google Scholar 

  • Hazarika A, Sarkar SN (2001) Effect of isoproturon pretreatment on the biochemical toxicodynamics of anilofos in male rats. Toxicology 165:87–95

    Article  CAS  PubMed  Google Scholar 

  • Hong JH, Lee IS (2009) Cytoprotective effect of Artemisia capillaries fractions on oxidative stress-induced apoptosis in V79 cells. BioFactors 35:380–388

    Article  CAS  PubMed  Google Scholar 

  • Huang W, Zhang J, Moore DD (2004) A traditional herbal medicine enhances bilirubin clearance by activating the nuclear receptor CAR. J Clin Invest 113:137–143

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hung HY, Kuo SC (2013) Recent studies and progression of Yin Chen Hao (茵陳蒿Yīn Chén Hāo), a long-term used traditional Chinese medicine. J Tradit Complement Med 3(1):2–6

    Article  PubMed Central  PubMed  Google Scholar 

  • Ihsan A, Wang X, Zhang W, Tu HG, Wang YL, Huang LL, Iqbal Z, Cheng GY, Pan YH, Liu ZL, Tan ZQ, Zhang YY, Yuan ZH (2013) Genotoxicity of quinocetone, cyadox and olaquindox in vitro and in vivo. Food Chem Toxicol 59:207–214

    Article  CAS  PubMed  Google Scholar 

  • Jia R, Cao L, Du J, Xu P, Jeney G, Yin G (2013) The protective effect of silymarin on the carbon tetrachloride (CCl4)-induced liver injury in common carp (Cyprinus carpio). In Vitro Cell Dev Biol Anim 49:155–161

    Article  CAS  PubMed  Google Scholar 

  • Jian JC, Wu ZH (2003) Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson). Aquaculture 218:1–9

    Article  Google Scholar 

  • Jian J, Wu Z (2004) Influences of traditional Chinese medicine on non-specific immunity of Jian carp (Cyprinus carpio var. Jian). Fish Shellfish Immunol 16:185–191

    Article  PubMed  Google Scholar 

  • Jiang LF (1997) Liver diseases in fish and its prevention and treatment. Fish Sci Technol Inf 24(4):188–192

    Google Scholar 

  • Kirschner LB (2004) The mechanisms of sodium chloride uptake in hyper regulating aquatic animals. J Exp Biol 2004:1439–1452

    Article  Google Scholar 

  • Koo HN, Hong SH, Jeong HJ, Lee EH, Kim NG, Choi SD et al (2002) Inhibitory effect of Artemisia capillarison ethanol-induced cytokines (TNF-α, IL-1α) secretion in Hep G2 cells. Immunopharmacol Immunotoxicol 24:441–453

    Article  CAS  PubMed  Google Scholar 

  • Kouadir M (2007) Study of hepatoprotective and anti-inflammatory effects of Long Dan Xie Gan decoction and their mechanisms. [M.S. thesis], Yangzhou University 23–50

  • Lee TY, Chang HH, Chen JH, Hsueh ML, Kuo JJ (2007) Herb medicine Yin–Chen–Hao–Tang ameliorates hepatic fibrosis in bile duct ligation rats. J Ethnopharmacol 109:318–324

    Article  PubMed  Google Scholar 

  • Li MM, Jiang T, Hang JC, Yao XS, Li YB (2008) Protective effect of Rhizoma anemarrhenae total flavone on liver injury in mice induced by acetic acid. Chin Tradit Herb Drug 39:252–255

    CAS  Google Scholar 

  • Liu D, Tang HF, Zhang SQ, Di Y, Meng J, Wang JW (2007a) Protective effect of concentrated tablet of Polygonum cuspidatum on liver injury in mice. Lishizhen Medicine and Materia Medica Research 18:3034–3035

    Google Scholar 

  • Liu ZD, Zhao XG, Yiu SL (2007b) Study progress in treatment of hepatic injury and prospect. Guide J TCM 10:81–87

    Google Scholar 

  • Liu J, Liu DJ, Qiao FF, Ni JN, Lv YJ, Bao ED (2008) Establishment of experimental animal model of chicken liver and kidney damages induced by carbon tetrachloride. J Nanjing Agric Univ 31:117–120

    CAS  Google Scholar 

  • Lu JT, Yang Y, Wei W, Chen ZM (2008) Protective effects of compound astragalus extract on chemical and immunological liver injury in mice. Chin J Inf Tradit Chin Med 1:32–34

    Google Scholar 

  • Luo DS, Zheng HH, Liu Q (2001) Experimental research of Huangqin elixation’s protective effect on liver injury induced by carbon tetrachloride in rats. J Xianning Med Coll 15:92

    Google Scholar 

  • Lv HZ, Huang SF, Cui JM, Li G, Piao GC (2007) Protective effect of ultra fine powder of Rhodiola sacra on acute injury in mice. J Med Sci Yanbian Univ 30:259–261

    Google Scholar 

  • Mase A, Makino B, Tsuchiya N, Yamamoto M, Kase Y, Takeda S et al (2010) Active ingredients of traditional Japanese (kampo) medicine, inchinkoto, in murine concanavalin A-induced hepatitis. J Ethnopharmacol 127:742–749

    Article  CAS  PubMed  Google Scholar 

  • Mei JL, Huang YF (2006) Studies on enzymatic mechanism of the liver-protective effect of BaoGan Jiedu Decoction on experimental hepatosis in anguilla. Acta Veterinaria et Zootechnica Sinica 37(12):1353–1359 (in Chinese)

    Google Scholar 

  • Mondal A, Karan SK, Singha T, Rajalingam D, Maity TK (2012) Evaluation of hepatoprotective effect of leaves of Cassia sophera linn. Evid Based Complement Alt Med, vol 2012 Article ID 436139, p 5

  • Sancho E, Ferna’ndez-Vega C, Ferrando MD, Andreu-Molinar E (2003) Eel ATPase activity as biomarker of thiobencarb exposure. Ecotoxicol Environ Saf 56:434–441

    Article  CAS  PubMed  Google Scholar 

  • Shang YP, Fang SY, Ge JF, Zhang L, Li J (2007) Protective action of cordyceps polysaccharides on immunological liver injury in mice. West China J Pharm Sci 22:654–655

    CAS  Google Scholar 

  • Wada L, Ou B (2002) Antioxidant activity and phenolic content of Oregon cranberries. J Agric Food Chem 50:3495–3500

    Article  CAS  PubMed  Google Scholar 

  • Wang S (1955) Eleven herbal formulas for jaundice. Waitai Miyao, People’s Medical, Beijing, China 14:6

  • Wang YZ (2010) The protective effect of polysaccharide of grifola frondosa on carbon tetrachloride induced liver injury and its mechanism. [M.S. thesis], Shandong University

  • Wang Q, Xiao D (2007) Protective effect of Mogrosides on chronic liver injury in rats. Guangxi J Tradit Chin Med 30:54–56

    Google Scholar 

  • Wang YC, Yang XL, Zhao RC, Li JS, Xue FQ, Liang JP, Xu ZZ, Du XL (1992) Study on cumulative toxicity of quinocetone in mice. J Tradit Chin Vet Med 5:14

    Google Scholar 

  • Wang LQ, Miao LC, Wang XJ (2001) The newest researching development and clinic application of Yinchenhao Tang. J Chin Tradit Herb 8(4):23–25 (in Chinese)

    CAS  Google Scholar 

  • Wang KY, Zhao DM, Geng Y, Huang XL, Liu GX (2004a) Study on hepatic cells apoptosis induced by olaquindox in Cyprinus carpio. J Fish China 28(6):733–737 (in Chinese)

    Google Scholar 

  • Wang XJ, Li TL, Sun H (2004b) Hepatoprotective effects of Yinchenhao Tang and its constituent absorbed into blood after oral administration. Chin Pharmacol Bull 20(2):239–240 (in Chinese)

    CAS  Google Scholar 

  • Wang KY, Geng Y, Huang XL (2005) Apoptosis of hepatic cell in common carp poisoned with olaquindox by flow cytometry (FCM). J Fish China 12(5):648–651 (in Chinese)

    CAS  Google Scholar 

  • Wang JH, Choi MK, Shin JW, Hwang SY, Son CG (2012) Antifibrotic effects of Artemisia capillaries and Artemisia iwayomogi in a carbon tetrachloride-induced chronic hepatic fibrosis animal model. J Ethnopharmacol 140:179–185

    Article  PubMed  Google Scholar 

  • Wang CG, Zhang T, Cui XM, Li S, Zhao XH, Zhong XH (2013) Hepatoprotective effects of a Chinese herbal formula, Longyin Decoction, on carbon-tetrachloride-induced liver injury in chickens. Hindawi Publishing Corporation. Evid Based Complement Alt Med vol 2013, Article ID 392743, p 9

  • WHO (World Health Organization) (1991) Toxicological evaluation of certain veterinary drug residues in food. Olaquindox. WHO Food Additives Series, No 27, no 701 on INCHEM

  • Xie B, Yu DJ, Cheng SM (2008) Protective effect of modified minor Radix bupleuri decoction on liver injury induced by paracetamol. Lishizhen Medicine and Materia Medica Research 19:130–131

    Google Scholar 

  • Xu L, Gao J, Wang Y, et al (2011) Myrica rubra extracts protect the liver from CCl4-induced damage. Evid Based Complement Altern Med vol. 2011, Article ID 518302, p 8

  • Xue CS, Yang QH, Liu J (2009) Protective effect of Jiangzhi Ninggan capsule on acute hepatic injury induced by d-galactosamine in mice. Lishizhen Medicine and Materia Medica Research 20:295–296

    Google Scholar 

  • Yang RF (2008) Establishment of mice liver injury model induced by DMN and protective effect of Chinese medicine on liver. [Doctor thesis], Guangzhou University of Chinese Medicine

  • Ye JD, Han YW, Yang YH, Liu HB, Lu DY, Zhao JW (2003) Effect of olaquindox on oxygen consumption, red blood cell number, hematocrit and dyskaryosis in erythrocytes in mirror carp Cyprinus carpio L. J Dalian Fish Univ 18(1):14–18 (in Chinese)

    CAS  Google Scholar 

  • Ye JD, Liu HB, Zhao JW, Lu TY, Yang YH (2005) Changes of body composition, plasma biochemical indexes and Na+, K+ -ATPase activity in gill after feeding diets with different doses of olaquindox to Cyprinus carpio L. J Huazhong Agric Univ 24(2):197–202 (in Chinese)

    CAS  Google Scholar 

  • Zhang T, Chen Q, Tang SS, Jin X, Zou JJ, Liu FY, Zhang S, Xiao XL (2010) Olaquindox weakened the antioxidant system in human hepatoma G2 cells. Carcinog Teratog Mutagen 22(5):1–5

    Google Scholar 

  • Zhang JJ, Du Z, Wang YJ, Nie FH, Zhu ZY (2011) Preparation of modified oriental wormwood decoction containing serum and it’s protective effect on necrotic hepatocyte injury model. Tianjin Pharm 23(1):3–6

    Google Scholar 

  • Zhao DX, Zhang CM, Tang SS, Wang CC, Zhang S, Xiao XL (2013) Autophagic process induced by olaquindox in HepG2 cells. Carcinog Teratog Mutagen 25(3):1–5

    CAS  Google Scholar 

  • Zhou XH, Zhong ZD, Zhong S, Chen J (2013) Effect of modified Yinchenhao decoction on the expression of TLR4 mRNA and protein in injury liver of rats. Chin J Exp Tradit Med Formul 19(15):239–242

    Google Scholar 

  • Zhu SM, Tang ZP (2008) Research progress of protective effect of Yinchenhao decoction on liver. Shanghai J Tradit Chin Med 42:73–74

    Google Scholar 

  • Zou JJ, Zhang T, Tang SS, Chen Q, Jin X, Chen KP, Xiao XL (2009) Oxidative damage of mitochondria by olaqindox in HepG2 cells. Carcinog Teratog Mutagen 21(5):1–5

    Google Scholar 

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Acknowledgments

This work was supported by the fund of Ankang Fisheries Experimental, Demonstration Building Project of Northwest A&F University (Z222020020), the Scientific Research Fund of Repatriation Personnel of Nation Education Commission and the Fundamental Research Funds for the Central Universities (Northwest A&F University, QN2011104).

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Authors and Affiliations

  1. Department of Fisheries Science, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Jishu Zhou & Hong Ji

  2. Station of China Fisheries Law Enforcement, Xi’an, 71200, Shaanxi, People’s Republic of China

    Chao Li

  3. Nutrition Source Institute of Beijing, Beijing Sunpu Biochemical and Technology Co., Ltd., Beijing, 100069, People’s Republic of China

    Lihong Wang

  4. Shaanxi Hua Qin Farming Technology Co., Ltd., Yangling, 712100, Shaanxi, People’s Republic of China

    Tianhe Zhu

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  1. Jishu Zhou
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  2. Chao Li
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  4. Hong Ji
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Correspondence to Hong Ji.

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Zhou, J., Li, C., Wang, L. et al. Hepatoprotective effects of a Chinese herbal formulation, Yingchen decoction, on olaquindox-induced hepatopancreas injury in Jian carp (Cyprinus carpio var. Jian). Fish Physiol Biochem 41, 153–163 (2015). https://doi.org/10.1007/s10695-014-0013-x

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