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Infection with Spiroplasma kunkelii on temperate and tropical x temperate maize in Argentina and development of a tool to evaluate germplasm

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Abstract

Corn stunt disease is a disease that extends from the southern United States to Argentina and is one of the most important diseases in warm subtropical areas of the continent, periodically causing yield reductions in maize. The aims of this study were to develop a severity scale, estimate the effect of corn stunt caused by Spiroplasma kunkelii in maize hybrids, and identify attributes related to yield loss that would be useful to evaluate germplasms. Under artificial inoculation conditions with infective and non-infective insects, symptoms were grouped to develop and validate a scale and a severity index. Disease severity, moment of appearance of the characteristic symptom, pathogen concentration, and effect on yield were correlated for eight temperate and temperate x tropical commercial and precommercial hybrid maize germplasms. We developed and validated a 7-score severity scale. The characteristic symptom of S. kunkelii infection occurs earlier in the temperate germplasm than in the tropical x temperate germplasm. The performance of two of the four temperate germplasms was remarkable compared to the hybrids. Severity and pathogen concentration were correlated with each other, and both correlated with yield and with yield reduction. The moment of appearance of the characteristic disease symptom did not correlate with the other attributes. The severity index and the pathogen concentration may be useful to evaluate germplasm.

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References

  • Alivizatos, A. S., & Markham, P. G. (1986). Acquisition and transmission of corn stunt spiroplasma by its leafhopper vector Dalbulus maidis. Annals of Applied Biology, 108(3), 535–544. https://doi.org/10.1111/j.1744-7348.1986.tb01992.x

    Article  Google Scholar 

  • Bajet, N. B. (1989). Occurrence of Corn Stunt Spiroplasma at different elevations in Mexico. Plant Disease, 73(11), 926. https://doi.org/10.1094/PD-73-0926

    Article  Google Scholar 

  • Bradfute, O. E., Tsai, J. H., & Gordon, D. T. (1981). Corn Stunt Spiroplasma and viruses associated with a maize disease epidemic in Southern Florida. Plant Disease, 65(10), 837. https://doi.org/10.1094/PD-65-837

    Article  Google Scholar 

  • Carloni, E., Carpane, P., Paradell, S., Laguna, I., & Giménez Pecci, M. P. (2013). Presence of Dalbulus maidis (Hemiptera: Cicadellidae) and of Spiroplasma kunkelii in the temperate region of Argentina. Journal of Economic Entomology, 106(4), 1574–1581. https://doi.org/10.1603/EC12323

    Article  CAS  PubMed  Google Scholar 

  • Caro, L., Carpane, P., Carloni, E., Santa, M., Laguna, I. G., & Giménez Pecci, M. P. (2006). Relación entre síntomas y serología y plantas de Maíz (Zea mays L.) infectadas experimentalmente con Spiroplasma kunkelii Whitcomb (pp. 304–305). XII Jornadas Fitosanitarias Argentinas.

  • Caro, L., Laguna, I. G., & Giménez Pecci, M. P. (2008). Ajuste de la escala que asocia síntomas y serología en maíz experimentalmente infectado con Spiroplasma kunkelii (p. 327). 1o Congreso Argentino de Fitopatología.

  • Carpane, P., Laguna, I. G., Virla, E. G., Paradell, S., Murua, L., & Giménéz Pecci, M. P. (2006). Experimental transmission of corn stunt spiroplasma present in different regions of Argentina. Maydica, 51, 461–468.

    Google Scholar 

  • Carpane, P., Melcher, U., Wayadande, A., Giménez Pecci, M. P., Laguna, G., Dolezal, W., & Fletcher, J. (2012). An Analysis of the Genomic Variability of the Phytopathogenic Mollicute <i>Spiroplasma kunkelii<i/>. Phytopathology, 103(2), 120926072324005. https://doi.org/10.1094/PHYTO-07-12-0158-R

    Article  CAS  Google Scholar 

  • Da Costa, R., Da Silva, D. D., Cota, L., Campos, L., Almeida, R., & Bernardes, F. (2019). Incidence of corn stunt disease in off-season corn hybrids in different sowing seasons. Pesquisa Agropecuária Brasileira, 54. https://doi.org/10.1590/s1678-3921.pab2019.v54.00872

  • De Oliveira, C. M., Lopes, J. R. S., Camargo, L. E. A., Fungaro, M. H. P., & Nault, L. R. (2007). No TitlGenetic diversity in populations of Dalbulus maidis (DeLong and Wolcott) (Hemiptera: Cicadellidae) from distant localities in Brazil assessed by RAPD-PCR Markers. Environmental Entomology, 36, 204–212.

    Article  Google Scholar 

  • De Oliveira, E., De Oliveira, C. M., Souza, I. R. P., Magalhães, P. C., & Cruz, I. (2002). Corn stuns: expression of leaf symptoms, detection of mollicutes and interactions with genotypes. Revista Brasileira de Milho e Sorgo, 1, 53–62.

    Article  Google Scholar 

  • De Oliveira, E., Landau, E., Guimarães, P. E., & Guimarães, L. J. M. (2010). Resistance of corn to stunting caused by spiroplasm and to stunting caused by phytoplasma. Asociación Brasileña de Maíz y Sorgo, 226.

  • De Oliveira, E., Resende, R., Giménéz Pecci, M. P., Laguna, I. G., Herrera, P., & Cruz, I. (2003). Incidência de viroses e enfezamentos e estimativa de perdas causadas por molicutes em milho no Paraná. Pesquisa Agropecuária Brasileira, 38(1), 19–25. https://doi.org/10.1590/S0100-204X2003000100003

    Article  Google Scholar 

  • Di Renzo, M. A., Bonamico, N. C., Díaz, D. D., Salerno, J. C., Ibañez, M. M., & Gesumaria, J. J. (2002). Inheritance of resistance to Mal de Río Cuarto (MRC) disease in Zea mays (L.). The Journal of Agricultural Science, 139(1), 47–53. https://doi.org/10.1017/S0021859602002241

    Article  Google Scholar 

  • Di Rienzo, J. A., Casanoves, F., Balzarini, M. G., Gonzalez, L., Tablada, M., & Robledo, C. W. (2020). InfoStatversión 2020. Centro de Transferencia (No. 2020). Centro de Transferencia InfoStat, FCA, Universidad Nacioinal de Córdoba, Argentina. www.infostat.com.ar

  • Díaz, C. G., Virla, E. G., Carloni, E., Giménéz Pecci, M. P., & Laguna, I. G. (2005). Evaluación preliminar del efecto de la fecha de siembra sobre la incidencia del corn stunt spiroplasma (CSS) (pp. 277–288). VIII Congreso Nacional de Maíz.

  • Galvão, S. R., Sabato, E. O., & Bedendo, I. P. (2021). Occurrence and distribution of single or mixed infection of phytoplasma and spiroplasma causing corn stunting in Brazil. Tropical Plant Pathology, 46(2), 152–155. https://doi.org/10.1007/s40858-020-00381-6

    Article  Google Scholar 

  • Giménéz Pecci, M. P., Carpane, P., Carloni, E., Nome, C. F., Fiorona, M., & Laguna, I. G. (2009). Técnicas empleadas en la identificación y caracterización de Spiroplasma kunkelii Withcomb (Reino Eubacteria, Clase Mollicutes). In I. G. Laguna, V. Conci, P. Rodriguez Pardina, G. Truol, M. Fiorona, & L. Di Feo (Eds.), Procedimientos empleados en la identificación de organismos fitopatógenos (pp. 53–65). Ediciones INTA.

  • Gimenéz Pecci, M. P., García, A., Druetta, M., Cabral, J. B., Ramirez, E. H., Raspanti, J., Ferrer, M., Maurino, M. F., & Laguna, I. G. (2014). ePeriodicidad y distribución de dos enfermedades del maíz (Zea mays L.) en la década del 2000 en Argentina (pp. 46–47). Escuela de Matemática Aplicada a La Biología-BIOMAT.

  • Giménéz Pecci, M. P., Laguna, I. G., Avila, A. O., De Remes Lenicov, A. M. M., Virla, E. G., Borgogno, C., Nome, C. F., & Paradell, S. (2002). Difusión del Corn Stunt Spiroplasma del maíz (Spiroplasma kunkelii) y del vector (Dalbulus maidis) en la República Argentina. Revista de La Facultad de Agronomía de La Plata, 105(1), 1–8.

    Google Scholar 

  • Gussie, J. S. (1995). Movement and multiplication of Spiroplasma kunkelii in Corn. Phytopathology, 85(10), 1093. https://doi.org/10.1094/Phyto-85-1093

    Article  Google Scholar 

  • Hidalgo, H., Jeffers, D., Castañon, G., & Rodriguez, F. (1998). Resistencia al achaparramiento del maíz mediante infestaciones de Dalbulus maidis en maíz. Agronomía MEsoamericana, 9(2), 119–124.

    Article  Google Scholar 

  • Hogenboom, N. G. (1993). Economic importance of breeding for disease resistance. In T. Jacobs & J. E. Parlevliet (Eds.), Durability of disease resistance. Current Plant Science and Biotechnology in Agriculture (pp. 5–9). Springer. https://doi.org/10.1007/978-94-011-2004-3_1

    Chapter  Google Scholar 

  • Lee, I. M. (1993). Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. Phytopathology, 83(8), 834. https://doi.org/10.1094/Phyto-83-834

    Article  CAS  Google Scholar 

  • Lenardon, S. L., Laguna, I. G., Gordon, D. L., Truol, G., Gómez, G., & Bratfute, O. E. (1993). Identification of Corn stunt spiroplasma in maize from Argentina. Plant Disease, 77(1), 100C. https://doi.org/10.1094/PD-77-0100C

    Article  Google Scholar 

  • Márquez Sánchez, F. (1982). The genetic improvement of resistance to the corn disease stunt and downy mildew in Nicaragua. Chapingo, 27, 26–27.

    Google Scholar 

  • Massola, N. S., Bebendo, I. P., Amorin, L., & Lopes, J. R. S. (1999a). Effects of the inoculation time on corn with Spiroplasma kunkelii on yield components. Fitopatologia Brasileira, 24, 571–573.

    Google Scholar 

  • Massola, N. S., Bebendo, I. P., Amorin, L., & Lopes, J. R. S. (1999b). Quantificação de danos causados pelo enfezamento vermelho e enfezamento pálido do milho em condições de campo. Fitopatologia brasileira, 24(2), 136–142 http://cat.inist.fr/?aModele=afficheN&cpsidt=10137411

    Google Scholar 

  • Mendoza, M., López, A., Rodriguez, S. A., Oyervides García, A., De León, C., & Jeffers, D. (2002). Acción génica de la resistencia al Achaparramiento del maíz causado por espiroplasma, fitoplasmas y virus. Revista Mexicana de Fitopatología, 20, 13–17.

    Google Scholar 

  • Nault, L. R. (1980). Maize Bushy Stunt and Corn Stunt: a comparison of disease symptoms, pathogen host ranges, and vectors. Phytopathology, 70(7), 659. https://doi.org/10.1094/Phyto-70-659

    Article  Google Scholar 

  • Oleszczuk, J. D., Catalano, M. I., Dalaisón, L., Di Rienzo, J. A., Giménez Pecci, M. P., & Carpane, P. (2020). Characterization of components of resistance to Corn Stunt disease. PLOS ONE, 15(10), e0234454. https://doi.org/10.1371/journal.pone.0234454

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pérez López, E., Olivier, C. Y., Luna Rodríguez, M., Rodríguez, Y., Iglesias, L. G., Castro Luna, A., Adame García, J., & Dumonceaux, T. J. (2016). Maize bushy stunt phytoplasma affects native corn at high elevations in Southeast Mexico. European Journal of Plant Pathology, 145(4), 963–971. https://doi.org/10.1007/s10658-016-0883-0

    Article  CAS  Google Scholar 

  • Ritchie, S. W., & Hanway, J. J. (1982). How a corn plant develops. Iowa State University of Science and Technology. Cooperative Extension Service Ames, Iowa. Special Report N° 48.

  • Sabato, E. O., Landau, E. C., Barros, B. A., & Oliveira, C. M. (2020). Differential transmission of phytoplasma and spiroplasma to maize caused by variation in the environmental temperature in Brazil. European Journal of Plant Pathology, 157(1), 163–171. https://doi.org/10.1007/s10658-020-01997-9

    Article  CAS  Google Scholar 

  • Scott, G. E., Rosenkranz, E. E., & Nelson, L. R. (1977). Yield loss of corn due to corn stunt disease complex 1. Agronomy Journal, 69(1), 92–94. https://doi.org/10.2134/agronj1977.00021962006900010024x

    Article  Google Scholar 

  • Sierra Macías, M., Becerra Leor, E., Palafox Caballero, A., Rodriguez Montalvo, F., Espinosa Calderón, A., & Valdivia Bernal, R. (2010). Tropical corn (Zea mays L.) genotypes with high yield and tolerance to corn stunt disease in the gulf of Mexico region. Tropical and Subtropical Agroecosystems, 12, 485–493.

    Google Scholar 

  • Silva, R. G., Galvão, J. C., Miranda, G. V., & De Oliveira, E. (2003). Controle genético da resistência aos enfezamentos do milho. Pesquisa Agropecuária Brasileira, 38(8), 921–928. https://doi.org/10.1590/S0100-204X2003000800004

    Article  Google Scholar 

  • Sutula, C. L. (1986). Interpreting ELISA data and establishing the positive-negative threshold. Plant Disease, 70(8), 722. https://doi.org/10.1094/PD-70-722

    Article  Google Scholar 

  • Teixeira, F. F., Costa, F. M., Sabato, E. O., Leite, C. E., Meirelles, W., Guimarães, C., & Belicuas, S. (2013). Pré-melhoramento de milho quanto à resistência a enfezamentos. Pesquisa Agropecuária Brasileira, 48(1), 51–58. https://doi.org/10.1590/S0100-204X2013000100007

    Article  Google Scholar 

  • Virla, E. G., Díaz, C. G., Carpane, P., Laguna, I. G., Ramallo, J., Gerónimo Gómez, L., & Giménez Pecci, M. P. (2004). Evaluación preliminar de la disminución en la producción de maíz causada por el " Corn stunt spiroplasma " ( CSS ) en Tucumán. Argentina. Boletín de Sanidad Vegetal. Plagas, 30, 403–413.

    Google Scholar 

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Acknowledgments

We are grateful to Sergio Uhart (Corteva Agriscience Argentina) and Irma Graciela Laguna (Consejo Nacional de Investigaciones Científicas y Técnicas – CONICET - INTA) for critical reading of the manuscript.

Funding

This work was supported by a grant from PD-E4-I090-001 (INTA).

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Authors and Affiliations

  1. Instituto de Patología Vegetal - Unidad de Fitopatología y Modelización Agrícola - Centro de Investigaciones Agropecuarias - Instituto Nacional de Tecnología Agropecuaria - Consejo Nacional de Investigaciones Científicas y Técnicas (IPAVE - UFYMA - CIAP - INTA – CONICET), Av. 11 de septiembre 4755, X5020ICA, Córdoba, Córdoba, Argentina

    Javier Miguel Barontini, Mariana Ferrer, Ada Karina Torrico, María Fernanda Maurino & María de la Paz Giménez Pecci

  2. Instituto de Tecnología Agroindustrial del Noroeste Argentino, Estación Experimental Agroindustrial Obispo Colombres – Consejo Nacional de investigaciones Científicas y Técnicas (ITANOA, EEAOC – CONICET), Av. William W. Cross 3150, C.P. T4101XAC, Las Talitas, Tucumán, Argentina

    Andrea Peña Malavera

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  1. Javier Miguel Barontini
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Barontini, J.M., Malavera, A.P., Ferrer, M. et al. Infection with Spiroplasma kunkelii on temperate and tropical x temperate maize in Argentina and development of a tool to evaluate germplasm. Eur J Plant Pathol 162, 455–463 (2022). https://doi.org/10.1007/s10658-021-02415-4

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