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Occurrence of Xiphinema santos Lamberti, Lemos, Agostinelli & D’Addabo 1993 (Nematoda: Longidoridae), a X. americanum-group member in Iran

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Abstract

A population of Xiphinema americanum-group was recovered in association with stone fruit trees in Isfahan province, center of Iran. A reverse taxonomic approach based upon the large subunit ribosomal DNA (LSU rDNA D2-D3) and the mitochondrial cytochrome c oxidase subunit I (COI mtDNA) gene sequences in integration with morphological studies, revealed that the recovered population belongs to Xiphinema santos. The Iranian population was mainly characterized by 1240–1868 μm long females with 60–84 μm long odontostyle, a = 37.2–51.9 and c = 42.8–54.6. It is further characterized by a lip region having a depression in junction with the body, presence of visible endosymbiont bacteria in ovaries under light microscope, dorsally convex and ventrally slightly concave conical tail with a blunt tip and three juvenile developmental stages. This population was similar to the type population in its morphology; however overlapped and extended morphometric data ranges, as well as differences in some indexes were observed. Compared to a Spanish population of this species, the Iranian population had a close morphology, similar morphometric data ranges and identical LSU and COI sequences. In LSU phylogeny, the relationship between the present and some previously sequenced isolates of the species and some isolates of three species X. georgianum, X. laevistriatum and X. citricolum was not resolved. In COI phylogeny, the clade of the Iranian and Spanish populations appeared as an independent lineage inside an unsupported clade including several species. The comparison with other populations of the species was reported and discussed. A second species, X. primum, that is native to Iran, was recovered from a new locality and characterized molecularly.

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References

  • Archidona-Yuste, A., Navas-Cortés, J. A., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., & Castillo, P. (2016). Cryptic diversity and species delimitation in the Xiphinema americanum-group complex (Nematoda: Longidoridae) as inferred from morphometrics and molecular markers. Zoological Journal of the Linnean Society, 176, 231–265.

    Article  Google Scholar 

  • Bowles, J., Blair, D., & McManus, D. P. (1992). Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology, 54, 165–174.

    Article  CAS  Google Scholar 

  • Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution, 17, 540–552.

    Article  CAS  Google Scholar 

  • De Grisse, A. T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nematodes phytoparasitaires. Mededelingen Rijksfakulteit Landbouwwetenschappen. Gent, 34, 351–369.

    Google Scholar 

  • de Man J. G. (1880). Die einheimischen, frei in der reinen Erde und im su ¨ssen Wasser lebende Nematoden, monographischen bearbeitet. Tijdschrift Nederlandse Dierkundige vereniging. 104 p.

  • Filipjev, I. N. (1934). The classification of free-living nematodes and their relations to parasitic nematodes. Smithsonian Miscellaneous Collection, 89, 1–63.

    Google Scholar 

  • Gutiérrez-Gutiérrez, C., Cantalapiedra-Navarrete, C., Decraemer, W., Vovlas, N., Prior, T., Rius, J. E. P., & Castillo, P. (2012). Phylogeny, diversity, and species delimitation in some species of the Xiphinema americanum-group complex (Nematoda: Longidoridae), as inferred from nuclear and mitochondrial DNA sequences and morphology. European Journal of Plant Pathology, 134, 561–597.

    Article  Google Scholar 

  • He, Y., Subbotin, S., Rubtsova, T. V., Lamberti, F., Brown, D. J. F., & Moens, M. (2005). A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology, 7, 111–124.

    Article  CAS  Google Scholar 

  • Huson, D. H., & Scornavacca, C. (2012). Dendroscope 3: An interactive tool for rooted phylogenetic trees and networks. Systematic Biology, 61, 1061–1067.

    Article  Google Scholar 

  • Jahanshahi Afshar, F., Pourjam, E., & Pedram, M. (2019). Lobocriconema iranense (Van den berg, Eskandari, Tiedt & Karegar, 2010) n. comb. and description of L. nokandense n. sp. (Nematoda: Criconematidae) from Iran. Nematology, 21, 1043–1061.

  • Jairajpuri, M. S. (1965). Qudsianema amabilis n. gen., n. sp. (Nematoda: Dorylaimoidea) from India. Proceedings of the Helminthological Society of Washington, 32, 72–73.

    Google Scholar 

  • Kiontke, K., Gavin, N. P., Raynes, Y., Roehrig, C., Piano, F., & Fitch, D. H. (2004). Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss. Proceedings of the National Academy of Sciences of the United States of America, 101, 9003–9008.

  • Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780.

    Article  CAS  Google Scholar 

  • Lamberti, F., & Bleve-Zacheo, T. (1979). Studies on Xiphinema americanum sensu lato with descriptions of fifteen new species (Nematoda, Longidoridae). Nematologia Mediterranea, 7, 51–106.

    Google Scholar 

  • Lamberti, F., Lemos, R. M., Agostinelli, A., & D’Addabo, T. (1993). The Xiphinema americanum-group in the vineyards of the Dao and Douro regions (Portugal) with description of two new species (Nematoda, Dorylaimida). Nematologia Mediterranea, 21, 215–225.

    Google Scholar 

  • Lamberti, F., Bravo, M. A., Agostinelli, A., & Lemos, R. M. (1994). The Xiphinema americanum-group in Portugal with descriptions of four new species (Nematoda, Dorylaimida). Nematologia Mediterranea, 22, 189–218.

    Google Scholar 

  • Lamberti, F., Agostinelli, A., & Radicci, V. (1996). Longidorid nematodes from northern Egypt. Nematologia Mediterranea, 24, 307–339.

    Google Scholar 

  • Lamberti, F., Iovev, T., Choleva, B., Brown, D. J. F., Agostinelli, A., & Radicci, V. (1997). Morphometric variation and juvenile stages of some longidorid nematodes from Bulgaria with comments on the number of juvenile stages of Longidorus africanus, L. closelongatus and Xiphinema santos. Nematologia Mediterranea, 25, 213–237.

  • Larget, B., & Simon, D. L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution, 16, 750–759.

    Article  CAS  Google Scholar 

  • Lazarova, S. S., Malloch, G., Oliveira, C. M. G., Hübschen, J., & Neilson, R. (2006). Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations. Journal of Nematology, 38, 404–410.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lazarova, S., Peneva, V., & Kumari, S. (2016). Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae). ZooKeys, 574, 1–42.

  • Loof, P. A. A., & Coomans, A. (1972). The oesophageal gland nuclei of Longidoridae (Dorylaimida). Nematologica, 18, 213–233.

    Article  Google Scholar 

  • Luc, M. (1958). Xiphinema de l'ouest Africain: Description de cinq nouvelles espèces (Nematoda; Dorylaimidae). Nematologica, 3, 57–72.

  • Micoletzky, H. (1927). Neue und seltene freilebende Nematoden aus dem Wolgagebiet (Kama). Zoologischer Anzeiger, 73, 113–123.

  • Mobasseri, M., Hutchinson, M. C., Jahanshahi Afshar, F., & Pedram, M. (2019). New evidence of nematode-endosymbiont bacteria coevolution based on one new and one known dagger nematode species of Xiphinema americanum-group (Nematoda, Longidoridae). PLoS One, 14, e0217506.

    Article  CAS  Google Scholar 

  • Nunn, G. B. (1992). Nematode molecular evolution: an investigation of evolutionary patterns among nematodes based upon DNA sequences. Ph.D. Thesis, University of Nottingham, Nottingham, UK.

  • Nylander, J. A., Ronquist, F., Huelsenbeck, J. P., & Nieves-Aldrey, J. L. (2004). Bayesian phylogenetic analysis of combined data. Systematic Biology, 53, 47–67.

    Article  Google Scholar 

  • Orlando, V., Chitambar, J. J., Dong, K., Chizhov, V. N., Mollov, D., Bert, W., & Subbotin, S. A. (2016). Molecular and morphological characterisation of Xiphinema americanum-group species (Nematoda: Dorylaimida) from California, USA, and other regions, and co-evolution of bacteria from the genus Candidatus Xiphinematobacter with nematodes. Nematology, 18, 1015–1043.

  • Pedram, M. (2018). Nematodes of the family Longidoridae. In: Ghaderi, R., Kashi, L. & Karegar, A. (Eds). Plant-parasitic nematodes in Iran. Science reference in collaboration with the Iranian Society of Nematology, pp. 627–67. (In Persian).

  • Rambaut, A., & Drummond, A. J. (2009). Tracer version 1.5 [computer program] http://beast.bio.ed.ac.uk/.

  • Robbins, R. T., Brown, D. J. F., Halbrendt, J. M., & Vrain, T. C. (1996). Compendium of juvenile stages of Xiphinema species (Nematoda: Longidoridae). Russian Journal of Nematology, 4, 163–171.

  • Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

    Article  CAS  Google Scholar 

  • Thorne, G. (1935). Notes on free-living and plant-parasitic nematodes. II-higher classification groups of Dorylaimoidea. Proceedings of the Helminthological Society of Washington, 2, 96–98.

  • Thorne, G. (1939). A monograph of the nematodes of the superfamily Dorylaimoidea. Capita Zoological, 8, 261 pp.

  • Thorne, G. (1974). Nematodes of the Northern Great Plains. Part II. Dorylaimoidea in part (Nemata: Adenophorea). South Dakota State University Agriculture Experimental Station Technical Bulletin 41, 120 pp.

  • Thorne, G., & Allen, M. W. (1950). Paratylenchus hamatus n. sp, and Xiphinema index n. sp., two nematodes associated with fig roots, with a note on Paratylenchus anceps cobb. Proceedings of the Helminthological Society of Washington, 17, 27–35.

  • Whitehead, A. G., & Hemming, J. R. (1965). A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology, 55, 25–38.

    Article  Google Scholar 

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Acknowledgements

The financial support of Tarbiat Modares University is appreciated. The authors acknowledge the critically reading of the MS by Dr. Mohammad Reza Atighi. The presented findings in this paper are part of the results of the project number 0-16-16-94176 by the first author entitled “Determination of infestation of pomegranate orchards and nurseries to plant parasitic nematodes with an emphasis on root knot nematodes”.

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Authors and Affiliations

  1. Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Farahnaz Jahanshahi Afshar

  2. Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, 45371-38791, Iran

    Fatemeh Shahryari

  3. Department of Agronomy, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Ali Mokhtassi-Bidgoli

  4. Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Córdoba, Spain

    Pablo Castillo

  5. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Zeinab Mirzaie Fouladvand & Majid Pedram

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  1. Farahnaz Jahanshahi Afshar
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Correspondence to Majid Pedram.

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All the authors certify that they do not have any conflict of interest, the presented data are original and the presented data are not published, and are not under evaluation for publication elsewhere, all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected. They also certify that the final version of this manuscript has been reviewed by all authors.

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The soil samples analyzed in this study were collected under the direct monitoring of the garden owner or from the natural public regions that are not under protection against soil samplings.

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Jahanshahi Afshar, F., Shahryari, F., Mokhtassi-Bidgoli, A. et al. Occurrence of Xiphinema santos Lamberti, Lemos, Agostinelli & D’Addabo 1993 (Nematoda: Longidoridae), a X. americanum-group member in Iran. Eur J Plant Pathol 157, 281–291 (2020). https://doi.org/10.1007/s10658-020-01980-4

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