Skip to main content
SpringerLink
Log in

Association of Adenosine Deaminase, Superoxide Dismutase, and Catalase Activities with Helicobacter pylori

  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Our purpose was to investigate associations between adenosine deaminase (ADA), superoxide dismutase (SOD), and catalase (CAT) activities and H. pylori.Ninety-nine patients were studied. Eight antral mucosal biopsies were taken for biochemical assessment of ADA, CAT, AND SOD activity and histological assessment. H. pyloridensity wAS evaluated according to the updated Sydney system. Patients were divided into three groups according to Sydney classification. ADA activity was found to be higher in patients having H. pyloriin the present study. Also, ADA activity was higher in patients with a severe density of H. pylori. SOD level was found to be significantly higher with increased H. pyloridensity in our study (P< 0.05). In addition, SOD activity was higher in it H. Pylori-positive than H. pylori-negative patients. We did not find CAT activity in some antral tissue specimens. The significantly high levels of ADA activity in patients with H. pyloriinfection may reflect the regulator role of ADA in acid secretion. The higher ADA level with increased H. pyloridensity and H. pyloripositivity indicate the probable malign lymphoid process of the stomach. But these findings must be confirmed with larger studies that include different gastric lesions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bruer T, Malaty HM, Graham DY: The epidemiology of H pylori associated gastroduodenal disease. In The Immunobiology of H. pylori: From Pathogenesis to Prevention. Ernst PB, Michetti P, Smith PD (eds). Philadelphia, Lippincott–Raven, 1997, pp 1–14

    Google Scholar 

  2. Chen XY, Liu WZ, Shi Y, Zhang DZ, Xiao SD, Tytgat GNJ: Helicobacter pylori associated gastric diseases and lymphoid tissue hyperplasia in gastric antral mucosa. J Clin Pathol 55:133–137, 2002

    CAS  PubMed  Google Scholar 

  3. Wothespoon A. Doglionil C, Diss T, et al.: Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet 342:575–579, 1993

    Google Scholar 

  4. Bagchi D, McGinn TR, Ye X, Bagchi M, Krohn RL, Chatterjee A, Stohs SJ: Helicobacter pylori-induced oxidative stress and DNA damage in a primary culture of human gastric mucosal cells. Dig Dis Sci 47:1405–1412, 2002

    Article  CAS  PubMed  Google Scholar 

  5. Mazzucchelli L Blaser A, Kappeler A, et al.: BCA-1 higly expressed in helicobacter pylori-induced mucosa-associated lymphoid tissue and gastric lymphoma. J Clin Invest 104:R49–R54, 1999

    Google Scholar 

  6. Piras MA, Gakis C, Budroni M, Andreoni G: Adenosine deaminase activity in pleural effusions: an aid to differential diagnosis. Br Med J 2:1751–1752, 1987

    Google Scholar 

  7. Biberfeld G, Biberfeld P, Sterner G: Cell-mediated immune response following Mycoplasma pneumoniae infection in man. I. Lymphocyte stimulation. Clin Exp Immunol 17:29–41, 1974

    CAS  PubMed  Google Scholar 

  8. Young E, Taylor HR: Immune mechanisms in chlamydial eye infections: cellular immune responses in chronic and acute dizease. J Infect Dis 150:745–751, 1984

    CAS  PubMed  Google Scholar 

  9. Querol JM, Barbe F, Manresa F, Esteban C, Canete C: Low value of adenosine deaminase in tuberculous pleural effusions. Eur Respir J 3:586–587, 1990

    CAS  PubMed  Google Scholar 

  10. Durak I, Perk H, Kavutcu M, Canbolat O, Akyol O, Beduk Y: Adenosine deaminase, 5′ nucleotidase, xanthine oxidase, superoxide dismutase, and catalase activities in cancerous and noncancerous human bladder tissues. Free Rad Biol Med 16(6):825–831, 1994

    Article  CAS  PubMed  Google Scholar 

  11. Cristalli G, Costanzi S, Lambertucci C, Lupidi G, Vittori S, Volpini R, Camaioni E: Adenosine deaminase: Functional implications and different classes of inhibitors. Med Res Rev 21:105–128, 2001

    Article  CAS  PubMed  Google Scholar 

  12. Asahi M, Azuma T, Ito S, Ito Y, Suto H, Nagai Y, Tsubokawa M, Tohyama Y, Maeda S, Omata M, Suzuki T, Sasakawa C: Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells. J Exp Med 191:593–602, 2000

    Article  CAS  PubMed  Google Scholar 

  13. Bamba N, Nakajima S, Andoh A, Bamba M, Sugihara H, Bamba T, Hattori T: Stem cell factor expressed in human gastric mucosa in relation to mast cell increase in Helicobacter pylori–infected gastritis. Dig Dis Sci 47:274–282, 2002

    Article  CAS  PubMed  Google Scholar 

  14. Stein M, Rappuoli R, Covacci A: Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation. Proc Natl Acad Sci USA 97:1263–1268, 2000

    CAS  PubMed  Google Scholar 

  15. Dunn BE, Cohen H, Blaser MJ: Helicobacter pylori. Clin Microbiol Rev 10:720–741, 1997

    CAS  PubMed  Google Scholar 

  16. Blaser MJ, Berg DE: Helicobacter pylori genetic diversity and risk of human dizease. J Clin Invest 107:767–773, 2001

    CAS  PubMed  Google Scholar 

  17. Ito Y, Azuma T, Ito S, Suto H, Miyaji H, Yamazaki Y, Kato T, Kohli Y, Keida Y, Kuriyama M: Sequence analysis and clinical significance of the iceA gene from Helicobacter pylori strains in Japan. J Clin Microbiol 38:483–488, 2000

    CAS  PubMed  Google Scholar 

  18. Giusti G: Adenosine deaminase. In Methods of Enzymatic Analysis, 2nd ed. Bergmeyer MV (ed). New York, Academic Press, 1974, pp 1092–1098

    Google Scholar 

  19. Beutler E: Red Cell Metabolism, 2nd ed. New York, Grune and Stratton, 1975, pp 261–265

    Google Scholar 

  20. Fridovich I: Superoxide dismutase. Adv Enzymol 41:35–97, 1974

    CAS  PubMed  Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr A, Randall R: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951

    CAS  PubMed  Google Scholar 

  22. Genta RM, Robason Go, Graham DY: Simultaneous visualization of Helicobacter pylori and gastric morphology: a new stain. Hum Pathol 25:221–226, 1994

    CAS  PubMed  Google Scholar 

  23. Ota H, Genta RM: Morphological characterization of the gastric mucosa during infection with H Pylori. In The Immunobiolology of H pylori: From Pathogenesis to Prevention. Ernst PB, Michetti P, Smith PD (eds). Philadelphia, Lippincott–Raven, 1977, pp 15–28

    Google Scholar 

  24. Xiang Z, Bugndi M, Rappuoli: Helicobacter pylori host responses in peptic ulceration. Lancet 342:900–901, 1993

    Google Scholar 

  25. Wyatt JI, Rathbone BJ: Immune response of the gastric mucosa to Campylobacter pylori. Scand J Gastroenterol 142:44–49, 1988

    CAS  Google Scholar 

  26. Eidt S, Stolte M: Prevalence of lymphoid follicles and aggregates in Helicobacter pylori gastritis in antral and body mucosa. J Clin Pathol 46:832–835, 1993

    CAS  PubMed  Google Scholar 

  27. Genta RM, Hammer HW, Graham DY: Gastric lymphoid follicles in Helicobacter pylori infection: frequency, distribution, and response to triple therapy. Hum Pathol 24:577–583, 1993

    Article  CAS  PubMed  Google Scholar 

  28. Ren Z, Pang G, Lee R: Circulating T-cell response to helicobacter pylori infection in chronic gastritis. Helicobacter 5:135–141, 2000

    Article  CAS  PubMed  Google Scholar 

  29. Xia Y, Khatchikian G, Zweier JL: Adenosine deaminase inhibition prevents free radical mediated injury in the postischemic heart. J Biol Chem 271:10096–10102, 1996

    Article  CAS  PubMed  Google Scholar 

  30. Namiot Z, Rutkiewicz J, Stasiewicz J, Gorski J: Adenosine deaminase activity in the human gastric mucosa in relation to acid secretion. Digestion 45:172–175, 1990

    Article  CAS  PubMed  Google Scholar 

  31. Kojima O, Majima T, Uehara Y, Yamane T, Fujita Y, Takahashi T, Majima S: Alteration of adenosine deaminase activity in peripheral blood lymphocytes of patients with gastric cancer. Jpn J Surg 15:130–133, 1986

    Google Scholar 

  32. Durak I, Ormeci N, Akyol O, Canbolat O, Kavutcu M, Bulbul M: Adenosine deaminase, 5′-nucleotidase, xanthine oxidase, superoxide dismutase, and catalase activities in gastric juices from patients with gastric cancer, ulcer, and atrophic gastritis. Dig Dis Sci 39:721–728, 1994

    Article  CAS  PubMed  Google Scholar 

  33. Mori M, Suziki H, Suzuki M, Kai A, Miura S, Ishii H: Catalase and SOD secreted from Helicobacter pylori. Free Radic Res 24:439–450, 1996

    Google Scholar 

  34. Farkas R, Selmeci L, Tulassay Z, Pronai L: Superoxide-dismutase activity of the gastric mucosa in patients with Helicobacter pylori infection. Anticancer Res 23:4309–4312, 2003

    CAS  PubMed  Google Scholar 

  35. Vo TKO, Druez C, Delzenne N, Taper HS, Poberfroid M: Analysis of antioxidant defence systems during rat hepatocarcinogenesis. Carcinogenesis 9:2009–2013, 1988

    CAS  PubMed  Google Scholar 

  36. Goldstein BD, Czerniecki B, Witz G: The role of free radicals in tumor promotion. Environ Health Perspect 81:55–57, 1989

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Surgery, KSU Faculty of Medicine, Kahramanmaras, Turkey

    E. Bulbuloglu MD (Prof, Dr.), R. Çağlar MD & T. Kale Ilhami MD

  2. Department of Biochemistry, KSU Faculty of Medicine, Kahramanmaras, Turkey

    F. Inanc MD & M. Kilinc MD

  3. Department of Pathology, KSU Faculty of Medicine, Kahramanmaras, Turkey

    S. Bakaris MD

  4. Department of Pathology Gastroenterology, KSU Faculty of Medicine, Kahramanmaras, Turkey

    B. Kantarceken MD & A Cetinkaya MD

  5. School of Medicine, Department of General Surgery, Kahramanmaras Sutcuimam University, 46050, Kahramanmaraş, Turkey

    E. Bulbuloglu MD (Prof, Dr.)

Authors
  1. E. Bulbuloglu MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Inanc MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Bakaris MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Kantarceken MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A Cetinkaya MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. Çağlar MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T. Kale Ilhami MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. Kilinc MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. Bulbuloglu MD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bulbuloglu, E., Inanc, F., Bakaris, S. et al. Association of Adenosine Deaminase, Superoxide Dismutase, and Catalase Activities with Helicobacter pylori . Dig Dis Sci 50, 2296–2299 (2005). https://doi.org/10.1007/s10620-005-3050-6

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10620-005-3050-6

KeyWords

Search

Navigation