Abstract
The present study aims to investigate the association between SOX4, Wnt signaling, and miRNAs under Wnt3 induction via bioinformatics analysis and functional essays. To briefly explore the expression of SOX4 protein in various types of cancer, we used ONCOMINE, a highly reputable cancer database, for comparison of its expression in prostate carcinoma relative to normal prostate gland. Concomitantly, we used CCLE to plot the copy number of SOX4 against its mRNA expression status in various cancerous cell lines to confirm the carcinogenesis role of SOX4. Afterward, whole profiling expression of microRNA in SOX4-stably expressed LNCaP cell line under the effect of Wnt3A were demonstrated. After identifying microRNA targets, STRING database and MIROB were used to explore the functional connection between proteins and microRNA with proteins. The results from our study shows that over-expressed of SOX4 was confirmed in both carcinogenesis tissue and cancer cell lines in Oncomine and CCLE database. In addition, five miRNAs, miR-16, miR-19a, miR320, miR-195, and miR-126, were differentially expressed in LNCaP cell line induced by Wnt3a. Pathway analysis of these targets proposed interaction networks of SOX4, Wnt3a with miR-126 and miR-195. Altogether, the miRNAs involved in Wnt and SOX4-mediated prostate cancer such as miR-126 and miR-195 could be potential biomarkers in prostate cancer.
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27 July 2020
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This study was supported by grants from the Chinese Culture University (PCCU 2017000162).
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Conceptualization: NNP; YHL; methodology: NNP; LYH; software: NNP; validation: LYH; formal analysis: NNP; LYH; investigation: NNP; LYH; resources: LYH; CSM; Data curation: NNP; Writing-original draft: NNP; LYH; writing—review and editing: NNP; LYH; visualization: NNP; LYH; supervision: LYH; CSM; project administration: CSM; LYH; funding acquisition: LYH.
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All procedures performed in studies involving genetic modification were in accordance with the ethical standards and issued by the ethics committee of Chinese Culture University (Number: CCU–106-002; Issue date: 21 December, 2017).
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Phan, N.N., Moreno, C.S. & Lai, YH. Overexpression of SOX4 induces up-regulation of miR-126 and miR-195 in LNCaP prostate cancer cell line. Cytotechnology 72, 527–537 (2020). https://doi.org/10.1007/s10616-020-00399-3
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DOI: https://doi.org/10.1007/s10616-020-00399-3