Abstract
This study fabricated a nano-biomaterial of supermagnetized α-cellulose (αCS@Fe3O4) for enhanced osteoconduction of hADSCs (human adipose-derived stem cells). First, the reduction precipitation method was successfully employed to synthesize αCS@Fe3O4. The synthesized material αCS@Fe3O4 was structurally and morphologically characterized by SEM, TEM, XRD, TGA, and XPS analyses. The characterizations confirmed a nanostructural modification of αCS using Fe3O4 nanoparticles. Next, the fabricated material αCS@Fe3O4 was assessed for biocompatibility. The obtained data confirmed the biocompatible nature of the αCS@Fe3O4. Then, the αCS@Fe3O4 was applied for the osteoconductive differentiation of the hADSCs. The hADSCs osteoconduction was enhanced significantly (11.325 fold increase) in the presence of the αCS@Fe3O4 compared to the control hADSCs. The Alizarin Red S (ARS) staining microscopic images corroborated the osteoconduction enhancement. Furthermore, the relative gene expression of the important osteogenic markers ALP, OCN, and RUNX2 was analyzed. The expression levels were significantly enhanced in the presence of the target material αCS@Fe3O4. Finally, the immunofluorescent staining of ALP, OCN, and RUNX2 corroborated the enhanced osteoconduction. Thus, in conclusion, αCS@Fe3O4 is a low-cost, economical, biocompatible nano-biomaterial with significant osteoconduction enhancement potential that can be applied in bone defect treatments in the future.
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Acknowledgments
The all authors acknowledge Dongguk University for providing all facilities. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2022R1A6A1A03053343), and the Rural Development Administration (S-2022-A0353-00004).
Funding
This work was carried out with the support by (S-2022-A0353-00004). This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2022R1A6A1A03053343).
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Supervision, AAK; Investigation, SCL, SHL, AAK; Methodology, SCL, AAK, DHK, MK, JSS; Writing-Original Draft, SCL, AAK; Writing—Review & Editing, SCL, AAK, JSS. All authors read and approved the final manuscript.
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Lee, SC., Lee, SH., Kang, DH. et al. Supermagnetic α-cellulosic nano-scaffolds for human adipose-derived stem cells osteoconduction enhancement. Cellulose 30, 2385–2398 (2023). https://doi.org/10.1007/s10570-023-05045-7
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DOI: https://doi.org/10.1007/s10570-023-05045-7