The translational potential of human induced pluripotent stem cells for clinical neurology

The induced pluripotent state represents a decade-old Nobel prize-winning discovery. Human-induced pluripotent stem cells (hiPSCs) are generated by the nuclear reprogramming of any somatic cell using a variety of established but evolving methods. This approach offers medical science unparalleled experimental opportunity to model an individual patient’s disease “in a dish.” HiPSCs permit developmentally rationalized directed differentiation into any cell type, which express donor cell mutation(s) at pathophysiological levels and thus hold considerable potential for disease modeling, drug discovery, and potentially cell-based therapies. This review will focus on the translational potential of hiPSCs in clinical neurology and the importance of integrating this approach with complementary model systems to increase the translational yield of preclinical testing for the benefit of patients. This strategy is particularly important given the expected increase in prevalence of neurodegenerative disease, which poses a major burden to global health over the coming decades.


Introduction
Medicine has evolved a sophisticated taxonomic repertoire that is based upon particular constellations of predominantly clinical and macroscopic/imaging features. Although such an approach has clear utility in medical practice, it leaves unresolved to some extent the cellular and molecular basis of disease. It follows that a complementary taxonomic (re)classification to reflect underlying-and potentially therapeutically targetable-molecular mechanisms may serve to strengthen both our diagnostic and therapeutic capacities. In order to first gain accurate insight into the molecular basis of human disease, it is crucial to employ an integrated approach that recognizes inherent limitations in each model system when used in isolation. Animal models and human non-neuronal cell lines have provided invaluable insight into developmental and translational neuroscience. Yet their success in identifying novel and clinically impactful therapies has been underwhelming, perhaps reflecting an inability to capture the true complexity of human neurological disease by using these approaches in isolation. Advances over the last decade have transformed the landscape of mechanistic evaluation and drug discovery in neurodegenerative disease, with new technologies permitting the study of previously inaccessible human cellular subtypes. The induced pluripotent state represents a Nobel prize-winning discovery made in the laboratory of Shinya Yamanaka in Kyoto, Japan, in 2006(Takahashi and Yamanaka 2006Takahashi et al. 2007). Human-induced pluripotent stem cells (hiPSCs) are generated by the nuclear reprogramming of any somatic cell using either genome integrating or non-integrating Bfootprint-free^methods (reviewed in Gonzalez et al. 2011). This approach offers medical science considerable and unprecedented experimental opportunities to model an individual patient's disease Bin a dish.^HiPSCs permit ontogenyrecapitulating directed differentiation into any human cell types, which themselves express donor cell mutation(s) at pathophysiological levels and thus hold considerable potential for disease modeling, drug discovery and potentially cell-based therapies.

Modeling neurodegeneration: the role of hiPSCs
The failure in clinical translation from preclinical models is particularly evident in the field of neurodegenerative disease, possibly arguing for the requirement of a human experimental system to complement-but not replace-existing models. While human postmortem tissue provides a valuable resource for investigating the end-stage pathological processes in a clinical disease, it does not allow dynamic insight into initiating molecular pathogenic events. HiPSCs possess two defining attributes: (i) the ability to self renew and (ii) the capacity to differentiate into any of the cell types comprising the organism from which they are derived. However, with this potential comes the complexity of directing differentiation into highly refined and regionally specified subtypes of neurons and glia (see Fig. 1).
This paradigm utilizes insights from developmental biology to rationalize a program of stage-specific morphogenetic instructions in order to predictably manipulate cell fate to desired lineages. These attributes-when considered with the fact that one can generate patientspecific hiPSCs-justify current enthusiasm about this technology to help elucidate cellular and molecular determinants of human disease. One major utility of this model is to resolve the functional cellular and molecular sequelae of monogenetic diseases, an approach that has now been validated by myriad studies including adultonset conditions. Indeed, well-characterized and developmentally rationalized protocols for neuronal and glial specification (reviewed elsewhere; Zirra et al. 2016;Goldman and Kuypers 2015;Tyzack et al. 2016;Patani 2016) have been employed on a range of patient-derived hiPSCs for both monogenic and sporadic neurodegenerative diseases to define disease-related phenotypes (see Table 1 for representative examples). The differentiation of clearly defined and functional cellular subtypes from stem cells provides the opportunity not only to elucidate but also to put into precise sequence the molecular steps culminating in cellular demise by conducting time-resolved experiments. However, in patients, these cells do not exist in isolation and, therefore, developing incrementally complex neuronal circuits, neuron-glia co-culture paradigms, and complex 3D Borganoids^are likely to deepen our understanding of these diseases (reviewed in Clevers 2016). RNA sequencing has been used to identify potential pathological pathways in an unbiased manner and, coupled with gene editing to correct a mutation, provide evidence that phenotypes detected are mutationdependent (Kiskinis et al. 2014;Reinhardt et al. 2013). Indeed, an experimental workflow for such an approach has recently been proposed (Merkle and Eggan 2013). Identification of early pathological processes-including post-transcriptional mechanisms of disease (Patani et al. 2012b)-offers the prospect therapeutic target definition for intervention at a presymptomatic/early symptomatic stage.

Maturational status of terminally differentiated neurons and glia
We and others have studied the maturity of terminally differentiated pluripotent stem cell-derived region-specific neurons using genome-wide gene expression and splicing analysis and found that they represent a fetal developmental state when compared to isotopic but anisochronic somatic counterparts (Patani et al. 2012a;Miller et al. 2013;Ho et al. 2016). This finding may bring into question their ability to model adult onset conditions. Yet, patient-specific hiPSCs have now been robustly shown across a range of neurogenetic conditions-summarized in Table 1-to possess the capacity to capture early pathogenic events in a mutation-and cell type-dependent manner. The ability to model adult-onset conditions in what is essentially a developmental system can be reconciled by the possibility that the in vivo environment (glial-neuronal interaction for example) may help to compensate cell autonomous neuronal dysfunction. Recognizing the ability of hiPSC derivatives to capture disease-relevant phenotypes then raises the interesting issue of how early the presymptomatic phase may actually begin (i.e., the duration of Bcompensated neuronal dysfunction^). Indeed, important insights have been gained by studying even neural precursors in certain familial forms of schizophrenia (Yoon et al. 2014), familial dysautonomia (Lee et al. 2012;Lee et al. 2009), and hereditary spastic paraparesis (Mishra et al. 2016), reinforcing the utility of this approach for elucidation of developmental phenotypes in pre-manifest disease states. In other cases, differentiated cell types have been exposed to stressors in order to elicit a phenotype (Reinhardt et al. 2013;Nguyen et al. 2011;Donnelly et al. 2013).
Can we do anything to accelerate aging of hiPSCderived neurons so they more faithfully represent their adult counterparts? Epigenomic changes including those in transcriptional and chromatin networks are observed with aging (Booth and Brunet 2016). Therefore, to comprehensively study neurodegenerative disease, it may be a complementary strategy to induce aging in hiPSC models as a useful comparator. To this end, experiments from Lorenz Studer's group have demonstrated that progerin-induced aging of hiPSCderived midbrain dopaminergic neurons to a more adult-like state is achievable . A more recent study from Fred Gage's laboratory systematically compared hiPSC-derived neurons with direct conversion/'transdifferentiation' from patients across a diverse age range to confirm that reprogramming Bresets^age, while transdifferentiation preserves donor age, thus highlighting the complementarity of these paradigms to study age-dependent phenotypes (Mertens et al. 2015). Indeed, direct conversion to multiple regionspecific and clinically relevant neuronal and glial subtypes has also been achieved (Son et al. 2011;Caiazzo et al. 2011;Yang et al. 2013a;Caiazzo et al. 2015), reinforcing the practical feasibility of comparative studies between direct conversion and hiPSC directed differentiation paradigms. A further interesting approach to inducing/accelerating aging of hiPSC-derived sympathetic neurons was their functional connection with Fig. 1 A schema depicting the generation of human-induced pluripotent stem cells from patient fibroblasts followed by sequential phases of lineage restriction. Directed differentiation paradigms can generate region-specific neural precursors, which can subsequently be differentiated into neurons, astrocytes, and oligodendrocytes. Diagrams were drawn using templates freely available from Servier Medical Art (http://www.servier.co. uk/content/servier-medical-art)  Dravet syndrome

SCN1A
Forebrain neurons (bipolar and pyramidal) Increased sodium currents using whole cell voltage and current clamp recordings.
Reduced threshold for action potential firing.  Rett syndrome

Neurons
Altered morphology, reduced glutamatergic synapse number. Altered calcium transients suggesting a deficiency in neuronal network connectivity. (Marchetto et al. 2010) cardiomyocytes (Oh et al. 2016). This study harnessed developmentally rationalized directed differentiation and in vivo circuitry to foster maturation status.

Genomic variation between hiPSC lines
Line-to-line variability continues to be a concern among stem cell biologists. However, significant advances in gene editing through zinc finger nucleases (ZFNs, Wood et al. 2011), transcriptional activator-like effector nucleases (TALENs, Boch et al. 2009), and more recently the development of the clustered regularly interspaced short palindromic repeats (CRISPR)-associated system (CRISPR/Cas9, Cong et al. 2013) allow the generation of isogenic controls to increase confidence in identifying mutation-dependent cellular and molecular phenotypes. These approaches and their practical utility in patient-specific hiPSCs have been recently reviewed elsewhere (Hendriks et al. 2016). Genome editing technologies are particularly relevant to monogenic diseases rather than complex genetic disorders or sporadic disease (Shribman et al. 2013;Samani et al. 2015;Patani et al. 2013;Athappily et al. 2013). An ideal approach for mendelian disorders is to generate reciprocal isogenic lines for a given mutation being studied-i.e., the mutation is corrected to create one isogenic pair, and it is separately inserted into a control line to generate a second isogenic pair ). An alternative approach to genome editing is to utilize larger numbers of control and mutant lines, but this has the potential to become prohibitively time-and resource-consuming, although feasibility has already been demonstrated in the context of a large and cohesive consortium (Consortium 2012). Generating isogenic controls, standardizing optimal differentiation protocols, and reproducing mutationdependent phenotypes across different laboratories are important considerations in future work. Additionally, intensive characterization of several lines derived from ethnically diverse control cases also has value to serve as a reference point, particularly if these lines are made available to other investigators. Such an approach would not only provide an invaluable resource but would also significantly reduce costs for stem cell researchers. Indeed, the hiPSC field has begun to achieve such stem cell repositories and had the foresight to include Bsecondary products^(e.g., reporter lines). This strategy promotes data sharing and comparison. Further attributes including integration-free lines, equal male and female line representation, and the prospect of intensively characterized cryopreservable differentiated precursors make such stem cell repositories an attractive prospect. Such non-profit initiatives also stand to benefit from close collaboration with industry.

Compound screening and drug discovery
Drug development is expensive and time-consuming, and those therapeutics that do emerge from preclinical studies have a low conversion rate into successful disease treatments due to limitations with either safety or efficacy. This failure of translation is particularly evident in the field of neurodegeneration, where only a very small percentage of drugs that reach the development phase are actually ever marketed (Ringel et al. 2013;Rubin 2008). Some putative reasons for this failure include (i) selection of diseases where the molecular pathogenesis is poorly understood, this precluding a mechanistically rationalized approach (ii) over reliance on one model system or the use of models that have poor predictive value of clinical success (e.g., due to interspecies differences, Peng et al. 2013;Seok et al. 2013;Scannell et al. 2012), (iii) candidate compounds that have poor safety profiles or (iv) pharmacodynamic properties (e.g., do not penetrate the blood-brain barrier), and (v) clinical trial design that is suboptimal due to patient heterogeneity and/or lack of appropriate end point measures.
The historical inaccessibility to most adult human neural cell types has consequently prevented their experimental integration into preclinical testing. In the last decade since their discovery, hiPSC models have already successfully identified promising compounds preclinically, some of which have since entered clinical trials (Wainger et al. 2014;Kiskinis et al. 2014). It is also noteworthy that drugs, which previously failed in expensive clinical trials, have also been shown to fail in relevant hiPSC models (Yang et al. 2013b), highlighting their utility/predictive power in preclinical testing. High throughput assays of patient-derived neurons and glia therefore offer the potential to transform preclinical testing when integrated with current in vivo approaches (see Fig. 2). Furthermore, due to one of their defining attributes of potentially limitless self-renewal, there is a theoretically unlimited supply of cellular material. Indeed, 3D culture techniques have been shown to allow the maturation of billions of uniform cells in a single flask (Rigamonti et al. 2016). Such scalability will in turn allow more comprehensive optimization of dosing regimen as well as concentration and duration of treatment through systematic fine-tuning, rather than the somewhat arbitrary dosing regimen currently in place for many drugs used in clinical medicine.
Characterizing a population of cryopreservable intermediate neural derivatives (e.g., region-specific precursors) would further improve scalability of cultures for high-content cell-based screening approaches. High-throughput screening has been used to identify compounds that can optimize directed differentiation of hiPSCs (Han et al. 2009;Maury et al. 2015) and demonstrates practical feasibility of employing such approaches for drug discovery. Indeed, this method has revealed important cell type-and species-specific effects, both in terms of toxicity and neuroprotective effects (Peng et al. 2013). A recent study performed a large-scale compound screen with hiPSC-derived neural precursor cells to decrease the Zika virus infection, demonstrating the utility of high-throughput approaches at the neural precursor stage . Some further representative examples of studies that have exploited hiPSC-derived neural precursors and/or neurons for drug discovery and toxicity assessment are provided in Table 2 below. The repertoire of live content readouts is steadily increasing; robust and commercially available assays include live/dead, neurite length/complexity, and mitochondrial integrity, to mention a few. The prospect of multiplexing these parameters in a human neuronal system is attractive from a disease modeling perspective.
Some drug discovery efforts have focused on Brepurposing^FDA-approved drugs. This approach allows significant reduction of time to clinical use. Conversely, a 5-8-year period is required from identifying a hit using conventional compound library screening due to further necessary medicinal chemistry/drug optimization. It is likely that in many diseases, particularly degenerative processes, combinations of drugs may prove necessary for optimal therapeutic effect. From this perspective, the field of neurodegeneration can glean some relevant insights into therapeutic strategy from cancer biology. Furthermore, different drugs might Fig. 2 A workflow for using human-induced pluripotent stem cell derivatives for disease modeling, drug discovery, and toxicity assays in high throughput. Diagrams were drawn using templates freely available from Servier Medical Art (http://www.servier.co. uk/content/servier-medical-art) Confirmed findings in second screen using hiPSC-derived neurons and fetal astrocytes with >80% showing cell specific toxicity. (Malik et al. 2014) be required either sequentially and/or combinatorially at distinct pathological Bphases^within the context of a disease (e.g., relapsing-remitting vs. secondary progressive multiple sclerosis). Within defined phenotypes at a particular disease phase, it is important to experimentally resolve the crucial pathogenic event(s) in order to guide therapy development around such validated targets specifically. The approach of screening compound libraries is important to inform which mechanistic phenomena are therapeutically manipulable. Important issues to address in the future (over and beyond the aforementioned stage-specific and combinatorial therapies) include screening compounds on enriched populations of specific cell types in mono-and coculture. Furthermore, systematic approaches to optimizing the duration of therapy, drug concentration, and timing of initiation relative to disease phase are all important considerations. Integrated medicinal chemistry expertise is then crucial to optimize the compound for clinical trial use. To this end, it is essential to ensure that any potential compound is safe in humans, which highlights another role for hiPSC derivatives as a predictive cell-based model for toxicity assessment. HiPSCs themselves do not faithfully capture the physiological attributes of clinically relevant somatic cell types (e.g., those of the liver, kidney, heart, and brain) and thus cannot serve as appropriate model for predictive toxicity assessment. However, using ontogeny-recapitulating directed differentiation of hiPSCs to desired cell types allows more accurate toxicity assessment in a relevant cellular context (see Fig. 2). Drug development attrition rates are high as current assays do not always reflect damage to hepatic, renal, cardiac, and neuronal cells at least partially due to species differences in metabolism. Hepatocytes from donor tissue have been used to model drug toxicity; however, they are limited by scarcity of donor tissue, and high-quality tissue which is available is usedquite rightly-for donation (Greenhough et al. 2010). Hepatocyte-like cells have been differentiated from hiPSCs with success by several groups (Siller et al. 2015). A generic hepatocyte library to test therapeutic drugs in high-throughput screening for liver toxicity would be an invaluable resource, along with similar approaches for other aforementioned organ systems. Optimized rapid differentiation protocols in the future may offer the opportunity for personalized toxicity screening, which in turn would permit the formulation of a bespoke therapeutic strategy accounting for a patient's own genetic polymorphisms.

Cellular therapy
Strategies for generating patient-specific neuronal and glial subtypes-through either reprogramming and directed differentiation or direct conversion methodshave fuelled excitement about the prospect of cellular therapy to restore structure and function in neurodegenerative diseases. Issues of safety are paramount in this context and broadly include rigorous tumorogenicity and immunogenicity testing, recently reviewed elsewhere (Xie and Tang 2016;Neofytou et al. 2015). Additionally practical feasibility includes assuring authenticity of cell fate, scalability, and enrichment (e.g., generating a billion cells >95% enriched for a particular neuronal subtype would be an approximate manufacturing benchmark). There are broadly two cellular sources that one can consider-autologous (theoretically removing the need for immunosuppression) or allogeneic/ BHLA matched.^It is also important to consider the goal of therapy at the cellular/molecular level-restoration of structure and function will require ontogenyrecapitulating differentiation of the cellular graft (e.g., authentic midbrain dopaminergic neurons in the case of Parkinson's disease). Conversely, implanted cells may invoke/strengthen endogenous mechanisms of repair through local effects on neighboring cells. Geron Corporation initiated the first human stem cell trial for spinal cord injuries in 2010, but unfortunately this was discontinued shortly afterwards for business-related strategic (rather than scientific) reasons (Scott and Magnus 2014;Lebkowski 2011). In order to illustrate the relevant principles of cellular therapy here, we have considered two examples in some detail; Parkinson's disease and age-related macular degeneration. Additional examples of relevant studies utilizing human iPSC-based cellular therapies for neurological disorders are then summarized in Table 3.
There is a significant history to cellular therapy in Parkinson's disease, particularly in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) primate model, which exhibits a characteristic loss of midbrain dopaminergic neurons in the substantia nigra and phenocopies the human condition. Transplantation of human fetal  neural stem cells into this model was found to ameliorate the disease phenotype. Noting that there is a proven relationship between graft survival and amelioration of the disease course (reversal of motor deficits), the findings within the same study of only a small minority of graft TH+ve donor cell survival required further explanation. Indeed, >95% remained as neural precursors and hence the mechanism of functional improvement was likely not to be exogenous cellular replacement, but rather influences of the graft on endogenous cellular function (Redmond et al. 2007;Bjugstad et al. 2008).
For reasons alluded to earlier, human pluripotent-including induced and embryonic-stem cells (hiPSCs/ hESCs) have several attractive features in this context including self-renewal and capacity for predictable manipulation using extrinsic developmentally rationalized cues. However, when hESC-derived midbrain dopaminergic neurons are implanted into a primate model of PD, disappointingly no TH+ve cells were again found to survive at postmortem analysis (Wakeman et al. 2014). Against this background, the authenticity of midbrain dopaminergic specification was re-evaluated. Indeed, it was previously demonstrated through a series of elegant experiments that FOXA2 is a key transcription factor for the specification of authentic midbrain dopaminergic neurons (Kittappa et al. 2007). The Studer lab next conducted groundbreaking work that built on this discovery, where authentic midbrain dopaminergic neurons (generated by first specifying floorplate cells rather than progression through a neural rosette paradigm) were transplanted into rodent and primate models of Parkinson's disease. Crucially, these interventions yielded both functional amelioration and survival of TH+ve/FOXA2+ve neurons 3 months post-transplant (Kriks et al. 2011). This represented a milestone achievement in this field. Subsequent work has considered implanting patient-specific hiPSC (rather than hESC)-derived midbrain dopaminergic (mDA) neurons. This was first tested in an MPTP primate model. Three months post-transplant, the mDA neurons mature in the host brain, retaining their BA9^molecular phenotype. It remains unresolved as to which source of dopaminergic neurons is optimal for transplantation into Parkinson's disease patients, and the field stands to benefit from direct comparison of fetal, hESC-, and hiPSC-derived cellular grafts in the context of longitudinal clinical and pathological follow-up studies (Brundin et al. 2010). Excitingly, the first hiPSC trial into human patients with age-related macular degeneration took place in 2014 where autologous hiPSC-derived retinal pigment epithelial cells were implanted (Chakradhar 2016). The intervention seemingly ameliorated progression in the first patient 1 year post-transplant, and no adverse effects were reported. However, quality checking of a second hiPSC line revealed mutations that were not seen in the somatic cells from which they were derived, consistent with previous reports (Gore et al. 2011). At this stage, the trial was suspended given unknown oncogenicity of the cell lines and costs of generating an autologous hiPSC line for each patient treated. The laboratory leading this work has confirmed that they will now turn their attention towards allogeneic hiPSCs (Garber 2015), which may indeed prove to be more cost-effective given that a total of approximately 150 preselected donors, (constituting a Bhaplobank^) could cater for the vast majority of the population in the UK (Taylor et al. 2012). The infrastructure here could follow basic design principles of blood banks, although an hiPSC haplobank would clearly require bespoke processes and quality control. It also presupposes equal potency between hiPSC lines, which is not always practically achieved (Hu et al. 2010;Boulting et al. 2011). Although the nervous system is an immunologically Bprivileged^site, the precise requirement for immunosuppression in neural grafting remains unresolved. There is therefore a balance to be struck between utilizing comprehensively characterized allogeneic lines versus Bpersonalized^autologous hiPSCs; the former seems to be a more practically and financially viable option in the immediate future. Further caution is required when one considers the potential toxicity of hiPSC-based cellular therapy specifically in the context of tumor formation. Reprogramming of somatic cells often utilizes lentiviral or retroviral strategies, raising the issue of random integration within the human genome of the host and related oncological consequences (Howe et al. 2008;Okita et al. 2007). Indeed, the process of directed differentiation itself may trigger a resurgence of exogenous transgene transcription (Okita et al. 2007). Some of this risk can be mitigated by using nonintegrating (and even non-viral) approaches to reprogramming. It is also possible that upon transplantation into a diseased environment, the hiPSC derivatives may be vulnerable to the disease for which they are being implanted. Such spread of disease into stem cell grafts has already been demonstrated in the context of fetal ventral mesencephalic transplantation in Parkinson's disease (Li et al. 2008). Indeed, similar concerns may exist with autologous transplantation of hiPSC derivatives, especially in genetic diseases. Recent advances in genome editing can at least partially temper this concern, where a specific mutation can be Bcorrected^in patient-specific iPSCs prior to autologous transplantation. Several varieties of customized nucleases are now established for genome editing, including zinc finger nucleases (ZFNs), transcription activator effector nucleases (TALENs), and the CRISPR-Cas9 system (Urnov et al. 2010;Sander and Joung 2014;Joung and Sander 2013). Significant optimization in both the design and quality checking of these approaches provides some early promise for their practical utility in regenerative medicine (Tsai and Joung 2014).
Current evidence suggests that cellular therapies can restore structure and function to some degree, but in order to be clinically tractable, they must first be shown to be superior to the current standard of care. Given that no disease-modifying therapies currently exist for most neurodegenerative diseases, it is the hope that cellular strategies may slow down, halt, or ideally reverse disease progression. An interesting idea to consider here is combinatorial therapy (e.g., cellular transplant plus GDNF infusion in the case of Parkinson's disease), which may be required to achieve clinically evident disease modification. Crucially, clinical trials need careful design including predefined end points, rigorous safety assessment, reproducible cellular derivation, tumorogenicity assays, and demonstration of preserved efficacy in the context of immunosuppression. To realize cellular therapies for neurodegenerative disease, it is clear that transparent international collaboration will be a key driving force.

Concluding remarks
HiPSCs hold tremendous promise for translational research in neurological disease (Connick et al. 2011). Although considerable advances have been made to date, these have not yet been optimally translated directly into improved patient care, which is the ultimate goal. Continuing advances in the directed differentiation of patient-specific hiPSCs into highly enriched populations of neuronal and glial subtypes will undoubtedly improve the precision of modeling cell type-specific phenotypes. There are certain regions of the neuraxis that have proved difficult to derive in vitro from hiPSCs such as the cerebellum (Wiethoff et al. 2015), although there have been recent promising advances . One important future goal is to conduct stagedefined transcriptome-wide analyses throughout human brain development from pluripotency through to adult old age and to include different neuraxial regions as they emerge and mature. This transcriptional atlas will, during its evolution, inform and guide directed differentiation strategies to multiple less well-studied neuraxial regions. Cell-based high-throughput screening methods for both ameliorating disease-specific phenotypes and toxicity assays will converge to reinforce a personalized approach to patient management. An important aspect to invest in for future studies is establishing salient (i.e., disease causing) phenotypes, which will necessitate discrimination of primary from secondary pathogenic events in time-resolved analyses. Functional genomic technologies with bespoke bioinformatics pipelines have proved powerful as unbiased approaches in Fig. 3 An organogram providing a framework within which human-induced pluripotent stem cells can be harnessed in the emerging discipline of regenerative neurology. Diagrams were drawn using templates freely available from Servier Medical Art (http://www.servier. co.uk/content/servier-medical-art) elucidating transcriptional phenotypes, which can then be followed up in a hypothesis-driven manner. These advances coupled together with robust genome editing technologies are converging to help realize the promise of hiPSCs in regenerative neurology (see Fig. 3).
In future, we envisage diagnostic and therapeutic integration of patient-specific hiPSCs into clinical management. As a first step, this might involve rapid reprogramming and differentiation of patient-specific cells followed by a personalized regimen of drugs selected through high-throughput screening with concurrent toxicity assays. How soon this future can become a reality depends not only on scientific advances but also on practical and financial feasibility. The prospect of automating the majority of hiPSC culture is crucial to ensure scalability and reproducibility across experiments. This would also reduce cell culture demands on researchers and the risk of infection. Such technologies are now available, although wider usage is likely limited by cost. Clinically impactful advances in the hiPSC field will undoubtedly be realized sooner through cohesive international consortia and the closer collaboration/colocation of industry with both academic and clinical colleagues, in order to drive drug discovery and translational neuroscience towards an era of precision medicine.
Consortium HDI. Induced pluripotent stem cells from patients