Abstract
Mostly, enzyme activity is greatly reduced after immobilization due to unfavourable conformational change occurred during the immobilization procedure. Herein, we report a novel magnetic nanoparticle-based platform for Bacillus altitudinis esterase (EstBASΔSP) immobilization using dialdehyde starch (DAS) as a molecular glue. First polydopamine (PDA) was coated on the surface of Fe3O4 nanoparticles (Fe3O4 NPs) with a controllable thickness. Thereafter, PDA-functionalized Fe3O4 NPs were modified with dialdehyde starch (DAS) to provide the aldehyde groups, which was employed as a glue to further fix the EstBASΔSP on particle surface via covalent bonding, resulting in the formation of a sea-urchin-like esterase-immobilized magnetic nanoparticle. The obtained nanoparticles (Fe3O4@PDA/DAS) achieved an enzyme load of 162.72 mg/g and retained 65.7% of its specific enzyme activity, demonstrating better thermal and storage stability compared with the “polydopamine-coated” nanoparticles (Fe3O4@PDA) and free EstBASΔSP. In addition, in a chloramphenicol palmitate synthesis, the immobilized esterase (EstBASΔSP-Fe3O4@PDA/DAS) gave 99% conversion and purity in 21 h (chloramphenicol: 0.15 M, enzyme dosage: 50 mg/mL) and retained over 80% of its activity after 12 cycles. This study provides a general strategy for immobilizing enzyme on nanoparticles and employs them as a novel platform for enzyme-mediated biocatalytic reaction.
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Acknowledgements
This research was financially supported by the National Natural Science Foundation of China (Grant No. C31570795), the Shanghai outstanding technical leaders plan 19XD1431800, the National Natural Science Foundation of China (Grant Nos. 81830052 and 81530053) and Shanghai Key Laboratory of Molecular Imaging (Grant No. 18DZ2260400).
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Dong, F., Lin, L., Su, Y. et al. Esterase-Immobilized Sea-Urchin-Like Fe3O4 Nanoparticles for Chloramphenicol Palmitate Synthesis. Catal Lett 153, 1974–1987 (2023). https://doi.org/10.1007/s10562-022-04136-7
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DOI: https://doi.org/10.1007/s10562-022-04136-7