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Methylation of promoter region of BRCA1 gene versus pathogenic variants of gene: risk factor or clinical marker of breast cancer

  • Preclinical study
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Abstract

Background

In this study, we compared the contribution of pathogenic variants of the BRCA1/2 genes (5382insC, 185delAG, 6174delT, 4153delA, T300G) and hypermethylation of the BRCA1 gene promoter region to the risk of breast cancer and clinical features in women.

Methods

This study enrolled 74 women (tumor tissue, blood) with newly diagnosed breast cancer and 62 women (blood) without oncological pathology (control group). Molecular genetic testing of samples and determination of hypermethylation status were performed on freshly collected material with the addition of a preservative before the procedure of DNA isolation.

Results

Hypermethylation of the BRCA1 gene promoter in women is a risk breast cancer factor (χ2 = 19.10, p = 0.001, OR = 16.25 (3.67–71.92)) and is more common than major pathogenic variants in the BRCA1/2 genes. The patients with the BRCA1 gene promoter hypermethylation were more likely to be diagnosed with late-stage metastatic cancer (χ2 = 4.31, p = 0.038, OR = 4.04 (1.19–13.65)). Hypermethylation of the BRCA1 gene promoter was predominant in tumor tissue among BC patients without family history compared to patients with cancer in relatives.

Conclusion

We proved that hypermethylation of the BRCA1 gene promoter is a risk factor for breast cancer and possibly an early biological marker of clinical onset, as its presence contributed to rapid disease progression with metastasis. The high frequency of hypermethylation in the examined breast cancer patients may be a consequence of environmental factors pressure on the risk of the disease development. Further large-scale studies are needed for the clinical application of the results.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Bogomolets National Medical University, Kyiv, Ukraine

    Olga Lobanova, Valeriy Cheshuk, Roman Vereshchako & Liubov Zakhartseva

  2. State Institution Reference-Center for Molecular Diagnostics of Public Health Ministry of Ukraine, Kyiv, Ukraine

    Nataliia Medvedieva, Liliia Fishchuk, Olha Dubitska & Zoia Rossokha

  3. Shupyk National Healthcare University of Ukraine, Kyiv, Ukraine

    Natalia Gorovenko

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  1. Olga Lobanova
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OL participated in the conceptualization, data acquisition, data curation, methodology, formal analysis, and writing of the original draft; NM participated in the conceptualization, methodology, investigation, writing of the original draft, preparation, and writing, reviewing, & editing of the manuscript; LF participated in the data acquisition, resources, and writing, reviewing, and editing of the manuscript; OD contributed to methodology and investigation; VC participated in the conceptualization and writing, reviewing, and editing of the manuscript; RV participated in the conceptualization and writing, reviewing, and editing of the manuscript; LZ participated in the conceptualization and writing, reviewing, and editing of the manuscript; ZR participated in the conceptualization, writing of the original draft, preparation, writing, reviewing, and editing of the manuscript, supervision, and project administration; NG participated in the conceptualization, writing, reviewing, and editing of the manuscript, supervision, and project administration. All authors read and approved the final manuscript.

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Correspondence to Liliia Fishchuk.

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Lobanova, O., Medvedieva, N., Fishchuk, L. et al. Methylation of promoter region of BRCA1 gene versus pathogenic variants of gene: risk factor or clinical marker of breast cancer. Breast Cancer Res Treat 196, 505–515 (2022). https://doi.org/10.1007/s10549-022-06774-2

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