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Biodegradation of PVCs through in-vitro identification of Bacillus albus and computational pathway analysis of ABH enzyme

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Abstract

Microplastics pose significant challenges to ecosystems and organisms. They can be ingested by marine and terrestrial species, leading to potential health risks and ecological disruptions. This study aims to address the urgent need for effective remediation strategies by focusing on the biodegradation of microplastics, specifically polyvinyl chloride (PVC) derivatives, using the bacterial strain Bacillus albus. The study provides a comprehensive background on the accumulation of noxious substances in the environment and the importance of harnessing biodegradation as an eco-friendly method for pollutant elimination. The specific objective is to investigate the enzymatic capabilities of Bacillus albus, particularly the alpha/beta hydrolases (ABH), in degrading microplastics. To achieve this, in-silico studies were conducted, including analysis of the ABH protein sequence and its interaction with potential inhibitors targeting PVC derivatives. Docking scores of − 7.2 kcal/mol were obtained to evaluate the efficacy of the interactions. The study demonstrates the promising bioremediation prospects of Bacillus albus for microplastics, highlighting its potential as a key player in addressing microplastic pollution. The findings underscore the urgent need for further experimental validation and practical implementation of Bacillus albus in environmental remediation strategies.

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Acknowledgements

Authors are thankful to the Researchers Supporting Project number (RSP2024R462), King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore, 54000, Pakistan

    Muhammad Naveed, Rida Naveed, Arooj Azeem, Mahrukh Afzal & Muhammad Waseem

  2. Department of Agriculture, University of Ioannina, Arta, Greece

    Tariq Aziz

  3. Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia

    Metab Alharbi, Abdulrahman Alshammari, Abdullah F. Alasmari & Thamer H. Albekairi

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  1. Muhammad Naveed
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Contributions

Conceptualization, MN and TA; methodology, RN and AA; software, MA; validation, MW; formal analysis, MAlharbi; investigation, AFA; resources, AA.; data curation, MA.; writing—original draft preparation, RN and AA.; writing—review and editing, THA; visualization, MN; supervision, MN and TA.; project administration, MN and TA; funding acquisition, MN and TA.

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Correspondence to Muhammad Naveed or Tariq Aziz.

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Naveed, M., Naveed, R., Aziz, T. et al. Biodegradation of PVCs through in-vitro identification of Bacillus albus and computational pathway analysis of ABH enzyme. Biodegradation (2024). https://doi.org/10.1007/s10532-023-10064-z

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