Cryptic alternative male mating strategies in invasive alien round goby (Neogobius melanostomus) of the upper Danube River

Male alternative mating strategies are a widespread life history choice, yet they have hardly been assessed in the context of fish invasions. As established proxies for alternative life-history strategies, body length and sexual maturity of Ponto–Caspian round goby (Neogobius melanostomus) from the recently invaded upper Danube River were contrasted and differential trophic niche occupation was assessed. Only about 4% of analyzed gobies qualified as potential sneakers which may be explained by the recent and ongoing invasion process. This study provides evidence for the occurrence of sneaking behavior of invasive round goby in the upper Danube River.


Introduction
Numerous and taxonomically widespread alternative reproductive strategies evolved within males, especially in species with (1) external fertilization, (2) egg guarding, or (3) high competition for a mating occasion (Taborsky et al. 2008). Their occurrence appears to be correlated with keen competition for mating opportunities, e.g. spawning localities or partners, and with alternative strategies paying off equally or higher concerning fitness. Sneaking, i.e. a parasitic alternative reproductive behavior used to exploit the reproductive investment of conspecific rival males, is a well-known alternative reproductive strategy (Taborsky et al. 2008). Sneakers have to invest in sperm quality and quantity, as they are confronted with high sperm competition during every mating (Taborsky et al. 2008). Taborsky (1998) described male sneaking behavior in 140 fish species of 28 families.
However, studies on alternative reproductive strategies have only recently started in the context of biological fish invasions (McCallum et al. 2019). The round goby, Neogobius melanostomus (Pallas 1811), has invaded numerous ecosystems worldwide (Kornis et al. 2012) and often became highly abundant in invaded areas (e.g. Cerwenka et al. 2018). Reproductively active males are aggressive, defending territories or potential mating partners against intra-and interspecific competitors (Kornis et al. 2012). Females preferentially mate with larger (Stammler and Corkum 2005) and darker males (Yavno and Corkum 2011), or those combining both features (Kodric-Brown 1990).
In round goby males, two behavioral reproductive strategies can be distinguished, the parental and the sneaker strategy (Taborsky et al. 2008; Table 1). Parental males (conventional, type I, or bourgeois males) primarily invest in somatic growth, and they occupy and defend territories. In contrast, sneaker males (type II or parasitic males) preferentially invest in reproduction at the cost of growth; they do not express secondary sexual characters, are small-sized, and their coloration resembles females. Sneakers try to hide their male status by imitating females to fertilize foreign conspecific spawns (Taborsky et al. 2008). Sneaker round gobies have a light mottled coloration, larger accessory sperm glands, a higher sperm volume, reduced 11-ketotestosterone levels (all: Marentette et al. 2009), significantly larger testes, and heavier otoliths (both: Bose et al. 2018). In the upper Danube River in Germany, round goby invasion processes, including spread, autecology, and impact on other species are well-studied (Brandner et al. 2013a(Brandner et al. , b, 2015Cerwenka et al. 2014Cerwenka et al. , 2017Cerwenka et al. , 2018. Based on evidence from many river systems worldwide, we hypothesize accordingly that round goby alternative male mating strategies are present there, too. Male round gobies (n = 312) were collected by electrofishing (ELT62-IID; Grassl GmbH, Berchtesgaden, Germany) from shorelines in a water depth of * 60 cm in 2010 (March 29-October 18) and 2011  at ten stretches along a 200 km section of the upper Danube River. Sex was determined based on the specific shape of the urogenital papilla (Marentette et al. 2009). Total length (L T ) of investigated specimens ranged from 3.7 to 15.2 cm (mean = 9.7 cm, SD = 1.5 cm).
To assign candidate sneaker males, body size (i.e. L T ) and energetic investment into reproduction, estimated as the gonado-somatic index were contrasted. The GSI was calculated as GSI = 100 9 M gonads-9 M S -1 , where M gonads is the weight of testes and seminal vesicles, and M S the weight of the eviscerated goby body. For doing so, (1) a piecewise regression (Ingle et al. 2015) of these two indices was used to identify dissimilar trends in different sections, i.e. a trend towards more male reproductive morphs; (2) a simplified threshold regression was applied to determine the particular L T at which the GSI separates these groups; and (3) finally, the lowest cut off of logtransferred GSI and the total sum of squares at both sides of morph groups determined the L T in combination with the GSI-threshold of 3% following Bleeker et al. (2017). Individuals not assignable to either sneakers or parental morphs were specified as intermediates (Table 2).
Individual trophic niche assignment of male round goby specimens was conducted using stable isotope analyses. Muscle tissue (0.5-1.0 cm 3 , Brandner et al. 2015) was used to determine the specimens lipid content and to quantify the ecological differentiation of alternative reproductive strategies by stable isotope ratios of d 13 C and d 15 N.
Male round goby GSI varied between 0 and 9.6% and the calculated sneaker L T cut-off was 102 mm. 14 (4%) specimens were classified as sneaker morphs. In contrast, 113 (36%) specimens were classified as parental morphs and 185 (59%) as intermediates. Male morph assignment was significantly correlated with divergent trophic niche occupation: lipid content was lowest in parental males and highest in sneaker males (

Discussion
This study provides evidence for the occurrence of alternative male mating strategies in round goby during their ongoing invasion of the upper Danube River (Table 2). Although this finding is novel in the context of a biological invasion process in the upper Danube River, alternative male mating strategies had been inferred for other round goby invasions, but with substantially different proportion estimates: whereas only about 4% round gobies were identified as sneakers in the invaded upper Danube River, much higher values of 40.6% in the River Rhine in the Netherlands (    are expected to occur less likely at the beginning of an invasion and are likely to become more numerous once the species is established. Accordingly, Kornis et al. (2012) suggest an increase in competition between males with increasing time since invasion and thus a likely increase of sneaker abundance after the carrying capacity of the invaded ecosystem is reached. Alternatively, different origins and genetic constitutions of invasive populations might affect male reproductive strategy distribution, too. Indeed, round goby populations of the upper Danube River differ genetically (Cerwenka et al. 2014), and it cannot be excluded that they have originated from different source populations; yet, there is no evidence that genetic differences are linked with alternative behavioral strategies. Furthermore, differences and limitations of study designs need to be considered: (1)  (4) sneaker male occurrence might vary with sampling season: Gertzen et al. (2016) reported sneaker males to occur until the end of European summer, whereas specimens in this study were collected from April through October. However, GSI values of round goby specimens did not significantly vary between months in this study (Bonferroni corrected Kruskal-Wallis test, all: p = 1); (5) individual hatching date may influence reproductive life history decisions (Bleeker et al. 2017), because males may have a smaller body size at the beginning of the first breeding season; and, finally, (6) mortality and migration propensities of morphs may vary according to different sneaker frequencies at collection spots (Marentette et al. 2010).
The individual trait hypothesis (Cerwenka et al. 2017) might provide a further explanation for alternative attributes within a population. Three of the candidate sneaker round goby specimens identified in the present study (21%) had outlying individual trait values in the study of Cerwenka et al. (2017): all three featured exceptional high lipid contents ([ 4.1, Table 3). Parentals might have increased metabolism and thus reduced energy storage amounting in lipid content though to nest defense, signaling, and courtship behavior (Svensson et al. 2004).
Sneaking seems not to be genetically determined but may be used in the early ontogeny of smaller-sized male round goby adults, primarily (Marentette et al. 2009). Its frequency is constrained by multiple different factors and thus the study design is the crucial factor for detecting this common behavioral alternative reproductive strategy.
Acknowledgements We thank all the friendly and helpful owners of the local fishing rights, the ''Fischereifachberatungen'' and Dr. G. Zauner who supported our sampling. Further, we want to thank Prof. Dr. Karl Auerswald and Dr. Rudi Schäufele (both: Technische Universitaet Muenchen) for processing stable isotope analyses and two anonymous reviewers for their helpful advice. This project was funded by the German Research Council DFG, project-number SCHL 567/5-1 and GE 2169/1-1 (AOBJ: 569812).
Funding Open Access funding enabled and organized by Projekt DEAL.
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