Abstract
The reproductive success of the round goby (Neogobius melanostomus), an invasive fish, may be mediated by the use of pheromones. We hypothesized that reproductive male (RM) round gobies release sex pheromone to which reproductive females (RF) respond. In this study, we compared behavioural and electrophysiological responses of reproductive and non-reproductive female round gobies to conspeci fic males. Results of behavioural experiments in the laboratory showed that RF spent significantly more time near the source of the male odour compared with odours from control water. However, RF did not distinguish between odours from non-reproductive male (non-RM) water and control water. Non-reproductive females (non-RF) were not attracted to odours released from RM or non-RM water. Results of electro-olfactogram (EOG) responses showed that both RF and non-RF discriminated between HPLC fractionated RM and non-RM odours. However, the EOG responses of RF were about eight-fold higher than non-RF exposed to RM odours. These findings confirm that RM round gobies release a pheromone signal that attracts RF. The results of this research may be useful in developing control strategy using natural pheromones to disrupt the reproductive behaviour of the invasive round goby and to curtail its effects on native species.
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References
Belanger RM (2002) Morphology and histochemistry of the peripheral olfactory organ and behavioural responses by the round goby, Neogobius melanostomus, to putative pheromones and conspecific extracts. MSc Thesis, Department of Biological Sciences, University of Windsor, 137 pp Belanger RM, Smith CM, Corkum LD and Zielinski B (2003)
RM Belanger CM Smith LD Corkum B Zielinski (2003) ArticleTitleMorphology and histochemistry of the peripheral olfactory organ in the round goby, Neogobius melanostomus (Teleostei: Gobiidae) Journal of Morphology 257 62–71 Occurrence Handle12740897 Occurrence Handle10.1002/jmor.10106
PM Charlebois LD Corkum DJ Jude C Knight (2001) ArticleTitleThe round goby (Neogobius melanostomus) invasion: current research and future needs Journal of Great Lakes Research 27 263–266
L Colombo PC Belvedere A Pilati (1977) ArticleTitleBiosynthesis of free and conjugated 5β-reduced androgens by the testis of the black goby, Gobius jozo L Bollettino di Zoologia 44 131–144
L Colombo PC Belvedere A Marconato (1979) ArticleTitleBiochemical and functional aspects of gonadal biosynthesis of steroid hormones in teleost fish Proceedings of the Indian National Academy of Sciences B 45 226–234
L Colombo A Marconato PC Belvedere C Frisco (1980) ArticleTitleEndocrinology of teleost reproduction: a testicular steroid pheromone in the black goby, Gobius jozo L Bollettino di Zoologia 47 355–364
LD Corkum MR Sapota KE Skora (2004) ArticleTitleThe round goby, Neogobius melanostomus, a fish invader on both sides of the Atlantic Ocean Biological Invasions 6 173–181 Occurrence Handle10.1023/B:BINV.0000022136.43502.db
DOL Dubs LD Corkum (1996) ArticleTitleBehavioral interactions between round gobies (Neogobius melanostomus) and mottled sculpins (Cottus bairdi) Journal of Great Lakes Research 22 838–844
M Fontaine (1938) ArticleTitleLa lamproie marine Sa peche et son importance economique. Bulletin de la Societe d’Oceanographie de France 17 1681–1687
SR Hall EL Mills (2000) ArticleTitleExotic species in large lakes of the world Aquatic Ecosystem Health and Management 3 105–135 Occurrence Handle10.1016/S1463-4988(99)00070-6
J Janssen DJ Jude (2001) ArticleTitleRecruitment failure of mottled sculpin Cottus bairdi in Calumet Harbor, Lake Michigan, induced by the newly introduced round goby Neogobius melanostomus Journal of Great Lakes Research 27 319–328
DJ Jude RH Reider GR Smith (1992) ArticleTitleEstablishment of Gobiidae in the Great Lakes basin Canadian Journal of Fisheries and Aquatic Sciences 49 416–421 Occurrence Handle10.1139/f92-047
P Karlson M Luscher (1959) ArticleTitle“Pheromones”: a new term for a class of biologically active substances Nature 183 55–56 Occurrence Handle13622694 Occurrence Handle1:STN:280:DyaG1M%2Flt1agsQ%3D%3D Occurrence Handle10.1038/183055a0
NI Kulikova (1985) ArticleTitleThe effect of chorionic gonadotropin in growth and maturation of the oocytes of the round goby, Neogobius melanostomus Journal of Ichthyology 25 86–98
Lee VA (2003) Factors regulating biomass and contaminant uptake by round gobies (Neogobius melanostomus) in western Lake Erie. MSc Thesis, Department of Biological Sciences, University of Windsor, 118 pp
W Li AP Scott MJ Siefkes HG Yan Q Liu SS Yun DA Gage (2002) ArticleTitleBile acids secreted by male sea lamprey that act as a sex pheromone Science 296 138–141 Occurrence Handle11935026 Occurrence Handle1:CAS:528:DC%2BD38XivVSqtbo%3D Occurrence Handle10.1126/science.1067797
AJ MacInnis LD Corkum (2000) ArticleTitleFecundity and reproductive season of the round goby Neogobius melanostomus in the upper Detroit River Transactions of the American Fisheries Society 129 136–144 Occurrence Handle10.1577/1548-8659(2000)129<0136:FARSOT>2.0.CO;2
PJ Miller (1984) The tokology of gobioid fishes GW Potts RJ Wooton (Eds) Fish Reproduction, Strategies and Tactics Academic Press London 119–153
HA Morrison DM Whittle GD Haffner (2000) ArticleTitleThe relative importance of species invasions and sediment disturbance in regulating chemical dynamics in western Lake Erie Ecological Modelling 125 279–294 Occurrence Handle1:CAS:528:DyaK1MXnslGlsbk%3D Occurrence Handle10.1016/S0304-3800(99)00189-1
CA Murphy NE Stacey LD Corkum (2001) ArticleTitlePutative steroidal pheromones in the round goby, Neogobius melanostomus: olfactory and behavioral responses Journal of Chemical Ecology 27 443–470 Occurrence Handle11441438 Occurrence Handle1:CAS:528:DC%2BD3MXksleitL4%3D Occurrence Handle10.1023/A:1010376503197
SJ Nichols G Kennedy E Crawford J Allen J French SuffixIII G Black M Blouin J Hickey S Chernyák R Haas M Thomas (2003) ArticleTitleAssessment of lake sturgeon ( Acipenser fulvescens) spawning efforts in the lower St Clair River, Michigan. Journal of Great Lakes Research 29 383–391
D Ottoson (1971) The electro-olfactogram LM Biedler (Eds) Handbook of Sensory Biology Springer-Verlag New York 95–131
WL Ray LD Corkum (2001) ArticleTitleHabitat and site affinity of the round goby Journal of Great Lakes Research 27 329–334 Occurrence Handle10.1016/S0380-1330(01)70648-X
AP Scott EL Vermeirssen (1994) Production of conjugated steroids by teleost gonads and their role as pheromones KG Davey RE Peter SS Tobe (Eds) Perspectives in Comparative Endocrinology National Research Council of Canada Ottawa 645–654
BR Smith JJ Tibbles (1980) ArticleTitleSea lamprey (Petromyzon marinus) in Lake Huron, Michigan, and Superior: history of invasion and control, 19361978 Canadian Journal of Fisheries and Aquatic Sciences 37 1780–1801 Occurrence Handle10.1139/f80-222
N Stacey P Sorensen (2002) Hormonal pheromones in fish D Pfaff A Arnold AM Etgen SE Fahrbach RT Rubin (Eds) Hormones, Brain and Behavior, Vol. 2, Non-mammalian Hormone-Behavior Systems Elsevier New York 375–434
GB Steinhart EA Marschall RA Stein (2004) ArticleTitleRound goby predation on smallmouth bass offspring in nests during simulated catch-and-release angling Transactions of the American Fisheries Society 133 121–131 Occurrence Handle10.1577/T03-020
WM Tavolga (1956) ArticleTitleVisual, chemical and sound stimuli as cues in the sex discriminatory behaviour of the gobiid fish Bathygobius soporator Zoologica-NY 41 49–65
C Vásárhelyi VG Thomas (2003) ArticleTitleAnalysis of Canadian and American legislation for controlling exotic species in the Great Lakes Aquatic Conservation Marine and Freshwater Ecosystems 13 417–427 Occurrence Handle10.1002/aqc.588
EL Vermeirssen AP Scott (1996) ArticleTitleMale priming pheromone is present in bile, as well as in urine, of female rainbow trout Journal of Fish Biology 58 1039–1045 Occurrence Handle10.1111/j.1095-8649.2001.tb00553.x
RG Wickett LD Corkum (1998) ArticleTitleNest defense by the non-indigenous fish, the round goby, Neogobius melanostomus (Gobiidae), on a shipwreck in western Lake Erie Canadian Field-Naturalist 112 653–656
TD Wyatt (2003) Pheromones and Animal Behaviour Communication by Smell and Taste Cambridge University Press Cambridge 391
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An erratum to this article is available at http://dx.doi.org/10.1007/s10530-006-1833-0.
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Corkum, L.D., Arbuckle, W.J., Belanger, A.J. et al. Evidence of a Male Sex Pheromone in the Round Goby (Neogobius melanostomus). Biol Invasions 8, 105–112 (2006). https://doi.org/10.1007/s10530-005-0333-y
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DOI: https://doi.org/10.1007/s10530-005-0333-y